Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   EL239_RS05500 Genome accession   NZ_LR134374
Coordinates   1265986..1266393 (-) Length   135 a.a.
NCBI ID   WP_126338719.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1260986..1271393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS05490 (NCTC12082_01116) letS 1261807..1264563 (+) 2757 WP_058482311.1 two-component system sensor histidine kinase LetS Regulator
  EL239_RS05495 (NCTC12082_01117) - 1264593..1265828 (+) 1236 WP_126338718.1 6-phosphofructokinase -
  EL239_RS05500 (NCTC12082_01118) pilA2 1265986..1266393 (-) 408 WP_126338719.1 pilin Machinery gene
  EL239_RS05505 (NCTC12082_01119) pilA2 1266501..1266911 (-) 411 WP_126338720.1 pilin Machinery gene
  EL239_RS05510 (NCTC12082_01120) - 1267264..1267581 (+) 318 WP_126338721.1 BolA family protein -
  EL239_RS05515 (NCTC12082_01121) - 1267574..1268017 (+) 444 WP_126338722.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  EL239_RS05520 (NCTC12082_01122) - 1268091..1269488 (+) 1398 WP_126339973.1 APC family permease -

Sequence


Protein


Download         Length: 135 a.a.        Molecular weight: 14226.55 Da        Isoelectric Point: 4.3285

>NTDB_id=1122399 EL239_RS05500 WP_126338719.1 1265986..1266393(-) (pilA2) [Legionella spiritensis strain NCTC12082]
MAIKGFTLIEFMIVVAIIAILVVIAIPFLQTYLIRARVSEGLELADPAKLAVTETAINTNVLPASQAQTGYVSPAATSYV
NSIIIDPGGVITITYTATAGGGTIIFMPTLQGNGDMTWDCSGGTLSDQYRPVNCR

Nucleotide


Download         Length: 408 bp        

>NTDB_id=1122399 EL239_RS05500 WP_126338719.1 1265986..1266393(-) (pilA2) [Legionella spiritensis strain NCTC12082]
ATGGCCATAAAAGGCTTTACCCTTATAGAATTTATGATAGTCGTTGCAATCATTGCCATCCTGGTTGTTATCGCTATTCC
CTTTTTGCAAACCTATCTGATAAGGGCTCGCGTGAGTGAAGGGCTGGAATTGGCAGACCCCGCCAAACTGGCCGTTACTG
AAACGGCTATCAATACCAACGTATTACCCGCCTCTCAGGCGCAAACCGGCTATGTCAGCCCTGCCGCAACATCCTATGTA
AATTCCATAATCATTGATCCCGGTGGTGTTATAACCATTACCTATACGGCAACGGCCGGTGGTGGCACTATTATCTTCAT
GCCCACGTTACAGGGTAATGGTGATATGACCTGGGATTGTTCCGGTGGTACGTTATCTGATCAGTATCGTCCGGTTAACT
GTCGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

71.852

100

0.719

  pilA2 Legionella pneumophila str. Paris

71.111

100

0.711

  pilA Ralstonia pseudosolanacearum GMI1000

40.625

100

0.481

  comP Acinetobacter baylyi ADP1

40.789

100

0.459

  pilA/pilA1 Eikenella corrodens VA1

33.758

100

0.393

  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.129

100

0.393

  pilA Haemophilus influenzae 86-028NP

40

96.296

0.385

  pilA Haemophilus influenzae Rd KW20

39.535

95.556

0.378

  pilA Pseudomonas aeruginosa PAK

33.557

100

0.37

  pilE Neisseria gonorrhoeae MS11

32.258

100

0.37


Multiple sequence alignment