Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL185_RS14835 Genome accession   NZ_LR134321
Coordinates   3430377..3431414 (-) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella baltica strain NCTC10737     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3425377..3436414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL185_RS14800 (NCTC10737_02979) - 3425483..3425800 (+) 318 WP_006085701.1 hypothetical protein -
  EL185_RS14805 (NCTC10737_02980) yciH 3425979..3426308 (+) 330 WP_126492445.1 stress response translation initiation inhibitor YciH -
  EL185_RS14810 (NCTC10737_02981) - 3426375..3426938 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  EL185_RS14815 (NCTC10737_02982) ruvX 3426968..3427393 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  EL185_RS14820 (NCTC10737_02983) hemH 3427482..3428495 (-) 1014 WP_126492446.1 ferrochelatase -
  EL185_RS14825 (NCTC10737_02984) - 3428528..3429067 (-) 540 WP_126492447.1 glutathione peroxidase -
  EL185_RS14830 (NCTC10737_02985) pilU 3429255..3430367 (-) 1113 WP_126493513.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL185_RS14835 (NCTC10737_02986) pilT 3430377..3431414 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  EL185_RS14840 (NCTC10737_02987) - 3431450..3432148 (+) 699 WP_126492448.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL185_RS14845 (NCTC10737_02988) proC 3432364..3433182 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  EL185_RS14850 (NCTC10737_02989) - 3433231..3433779 (+) 549 WP_006082520.1 YggT family protein -
  EL185_RS14855 (NCTC10737_02990) yggU 3433779..3434078 (+) 300 WP_126492449.1 DUF167 family protein YggU -
  EL185_RS14860 (NCTC10737_02991) - 3434188..3434622 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -
  EL185_RS14865 (NCTC10737_02992) rdgB 3434762..3435379 (+) 618 WP_126492450.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=1121457 EL185_RS14835 WP_006082517.1 3430377..3431414(-) (pilT) [Shewanella baltica strain NCTC10737]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1121457 EL185_RS14835 WP_006082517.1 3430377..3431414(-) (pilT) [Shewanella baltica strain NCTC10737]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAGATTAACCTGCCCGCGCTCGATCACCAAGGCGTACACAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCCGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTGGGTGCGCCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTCCTAGTGACTGGGCCAACGGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACACGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTACACCAAAATAAGCAATGTTTGATTAACCAGCGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTACGTGAGGATCCGGACGTGATTTTAGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCTGCAGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACCACCTCTGCCGCGAAAACCATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTGCGCACTATGTTGTCTGAATCGCTGCAAGCGGTTATTTCGCAAAC
CCTGATTAAAAAGGTAGGCGGAGGCCGTGTTGCCGCCCATGAAATCATGATGGGCACTCCCGCTATTCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCGGTATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment