Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL185_RS14830 Genome accession   NZ_LR134321
Coordinates   3429255..3430367 (-) Length   370 a.a.
NCBI ID   WP_126493513.1    Uniprot ID   -
Organism   Shewanella baltica strain NCTC10737     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3424255..3435367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL185_RS14795 (NCTC10737_02978) - 3424744..3425304 (-) 561 WP_006082509.1 DUF4136 domain-containing protein -
  EL185_RS14800 (NCTC10737_02979) - 3425483..3425800 (+) 318 WP_006085701.1 hypothetical protein -
  EL185_RS14805 (NCTC10737_02980) yciH 3425979..3426308 (+) 330 WP_126492445.1 stress response translation initiation inhibitor YciH -
  EL185_RS14810 (NCTC10737_02981) - 3426375..3426938 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  EL185_RS14815 (NCTC10737_02982) ruvX 3426968..3427393 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  EL185_RS14820 (NCTC10737_02983) hemH 3427482..3428495 (-) 1014 WP_126492446.1 ferrochelatase -
  EL185_RS14825 (NCTC10737_02984) - 3428528..3429067 (-) 540 WP_126492447.1 glutathione peroxidase -
  EL185_RS14830 (NCTC10737_02985) pilU 3429255..3430367 (-) 1113 WP_126493513.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL185_RS14835 (NCTC10737_02986) pilT 3430377..3431414 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  EL185_RS14840 (NCTC10737_02987) - 3431450..3432148 (+) 699 WP_126492448.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL185_RS14845 (NCTC10737_02988) proC 3432364..3433182 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  EL185_RS14850 (NCTC10737_02989) - 3433231..3433779 (+) 549 WP_006082520.1 YggT family protein -
  EL185_RS14855 (NCTC10737_02990) yggU 3433779..3434078 (+) 300 WP_126492449.1 DUF167 family protein YggU -
  EL185_RS14860 (NCTC10737_02991) - 3434188..3434622 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40990.12 Da        Isoelectric Point: 6.2352

>NTDB_id=1121456 EL185_RS14830 WP_126493513.1 3429255..3430367(-) (pilU) [Shewanella baltica strain NCTC10737]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSSRECNFAFAVKDLGR
FRVSAFWQRESSGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1121456 EL185_RS14830 WP_126493513.1 3429255..3430367(-) (pilU) [Shewanella baltica strain NCTC10737]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTTATCACAGCGGGTTTTCCGCCAAG
TGCTAAGATTGACGGTGAGTTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCGAGTCGTGAATGTAACTTCGCTTTTGCGGTAAAGGATTTAGGTCGC
TTTCGGGTAAGCGCCTTCTGGCAGCGTGAATCGTCAGGCTGCGTGATGCGCCGTATTGAGACTAAAATCCCTGACGTCGA
TGATTTAAAACTACCACCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGCCTCATCATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCAACATCTCTGGCGGCCTTGGTGGGATATCGTAATGGTCATGCTCGTGGTCATATTCTCACGATTGAAGAT
CCAGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAATCTTTCGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCGGAAACGGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAGCAGTTATTGTTCGATTTGTCATTGAATTTGCGCGGTATTGTGGCGCAGCA
ATTAATCCCAAAAGTCGATGGCACTGGGCGCCGCGCCGCGATTGAAGTATTGATCAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTGCTCAAAGAAACCATGGCTAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAGGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAATTACAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.677

100

0.6
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment