Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL200_RS11705 Genome accession   NZ_LR134301
Coordinates   2491970..2493007 (-) Length   345 a.a.
NCBI ID   WP_006422551.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain NCTC13014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2486970..2498007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL200_RS11680 (NCTC13014_02322) - 2487217..2487924 (-) 708 WP_014036228.1 YitT family protein -
  EL200_RS11685 (NCTC13014_02323) - 2488035..2488247 (-) 213 WP_129550566.1 DUF4287 domain-containing protein -
  EL200_RS11690 (NCTC13014_02324) - 2488574..2489473 (+) 900 WP_057501897.1 LysR family transcriptional regulator -
  EL200_RS11695 (NCTC13014_02325) - 2489536..2490519 (-) 984 WP_057501898.1 aldo/keto reductase -
  EL200_RS11700 (NCTC13014_02326) pilU 2490727..2491869 (-) 1143 WP_006422552.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL200_RS11705 (NCTC13014_02327) pilT 2491970..2493007 (-) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  EL200_RS11710 (NCTC13014_02328) - 2493095..2493772 (+) 678 WP_057501899.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL200_RS11715 (NCTC13014_02329) proC 2493791..2494612 (+) 822 WP_057501900.1 pyrroline-5-carboxylate reductase -
  EL200_RS11720 (NCTC13014_02330) - 2494672..2495547 (-) 876 WP_057501901.1 LysR family transcriptional regulator -
  EL200_RS11725 (NCTC13014_02331) - 2495619..2496248 (+) 630 Protein_2296 short chain dehydrogenase -
  EL200_RS11730 (NCTC13014_02332) soxR 2496322..2496756 (-) 435 WP_057501902.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6652

>NTDB_id=1120730 EL200_RS11705 WP_006422551.1 2491970..2493007(-) (pilT) [Stenotrophomonas maltophilia strain NCTC13014]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1120730 EL200_RS11705 WP_006422551.1 2491970..2493007(-) (pilT) [Stenotrophomonas maltophilia strain NCTC13014]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTTAAGAACAAAGCGTCCGACCTGCATCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCGTCGCTGGCGCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCATGGCTTCAATGA
AGCACTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAACTTCGTGACCTGGAAACGATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCTGAATCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCTGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment