Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL200_RS11700 Genome accession   NZ_LR134301
Coordinates   2490727..2491869 (-) Length   380 a.a.
NCBI ID   WP_006422552.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain NCTC13014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2485727..2496869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL200_RS11675 (NCTC13014_02321) - 2486601..2487143 (+) 543 WP_057499392.1 DNA-3-methyladenine glycosylase I -
  EL200_RS11680 (NCTC13014_02322) - 2487217..2487924 (-) 708 WP_014036228.1 YitT family protein -
  EL200_RS11685 (NCTC13014_02323) - 2488035..2488247 (-) 213 WP_129550566.1 DUF4287 domain-containing protein -
  EL200_RS11690 (NCTC13014_02324) - 2488574..2489473 (+) 900 WP_057501897.1 LysR family transcriptional regulator -
  EL200_RS11695 (NCTC13014_02325) - 2489536..2490519 (-) 984 WP_057501898.1 aldo/keto reductase -
  EL200_RS11700 (NCTC13014_02326) pilU 2490727..2491869 (-) 1143 WP_006422552.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL200_RS11705 (NCTC13014_02327) pilT 2491970..2493007 (-) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  EL200_RS11710 (NCTC13014_02328) - 2493095..2493772 (+) 678 WP_057501899.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL200_RS11715 (NCTC13014_02329) proC 2493791..2494612 (+) 822 WP_057501900.1 pyrroline-5-carboxylate reductase -
  EL200_RS11720 (NCTC13014_02330) - 2494672..2495547 (-) 876 WP_057501901.1 LysR family transcriptional regulator -
  EL200_RS11725 (NCTC13014_02331) - 2495619..2496248 (+) 630 Protein_2296 short chain dehydrogenase -
  EL200_RS11730 (NCTC13014_02332) soxR 2496322..2496756 (-) 435 WP_057501902.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42369.71 Da        Isoelectric Point: 6.7683

>NTDB_id=1120729 EL200_RS11700 WP_006422552.1 2490727..2491869(-) (pilU) [Stenotrophomonas maltophilia strain NCTC13014]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1120729 EL200_RS11700 WP_006422552.1 2490727..2491869(-) (pilU) [Stenotrophomonas maltophilia strain NCTC13014]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAACTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACCCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAATGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATCCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGCGCCACCGGCACCGGTAAATCGACGTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGACCCGATTGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
TGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCG
AACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTTTCGCTGAATCT
GAAGGGCGTGGTTGCGCAGCAGCTGATTCCTTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATTCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGTTACGAGGACGCGCTGCGCTACGCCGA
TTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCCTGTCGCAGGGGCTGGACGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.579

0.653
  pilU Acinetobacter baylyi ADP1

63.866

93.947

0.6
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403

Multiple sequence alignment