Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NITINOP_RS12160 Genome accession   NZ_LN885086
Coordinates   2582048..2583106 (-) Length   352 a.a.
NCBI ID   WP_062486236.1    Uniprot ID   A0A0S4KW38
Organism   Candidatus Nitrospira inopinata isolate ENR4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577048..2588106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NITINOP_RS12135 (NITINOP_2631) - 2577859..2578593 (-) 735 WP_062488030.1 intradiol ring-cleavage dioxygenase -
  NITINOP_RS12140 (NITINOP_2632) - 2578599..2579282 (-) 684 WP_062486224.1 Slp family lipoprotein -
  NITINOP_RS12145 (NITINOP_2634) - 2579298..2579921 (-) 624 WP_062486227.1 outer membrane protein -
  NITINOP_RS12150 (NITINOP_2635) - 2580318..2580794 (+) 477 WP_158023403.1 hypothetical protein -
  NITINOP_RS12155 (NITINOP_2636) pilU 2580843..2582018 (-) 1176 WP_062486233.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NITINOP_RS12160 (NITINOP_2637) pilT 2582048..2583106 (-) 1059 WP_062486236.1 type IV pilus twitching motility protein PilT Machinery gene
  NITINOP_RS12165 (NITINOP_2638) bioF 2583228..2584400 (-) 1173 WP_062486239.1 8-amino-7-oxononanoate synthase -
  NITINOP_RS12170 (NITINOP_2639) - 2584525..2585271 (+) 747 WP_062486242.1 tetratricopeptide repeat protein -
  NITINOP_RS12175 (NITINOP_2640) - 2585343..2586650 (-) 1308 WP_062486245.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38622.84 Da        Isoelectric Point: 6.7750

>NTDB_id=1115032 NITINOP_RS12160 WP_062486236.1 2582048..2583106(-) (pilT) [Candidatus Nitrospira inopinata isolate ENR4]
MDISKLLTFAVKEGASDCHLSAGEPPMVRIHGDMKKLDHPALTPEETHALIYDMMNDVQRKHFEEHRECDFSFELGDIAR
FRVNVFVQQRGLGAVFRNIPTIIVPLDKLGMPPVIRQLCDKEKGLILVTGPTGSGKSTTLAAMVDYLNTTFEGHIITIED
PIEFVHKSKKCLVNQRELGVHTLSFANALRSALREDPDIILVGEMRDLETIQLALTAAETGHLVFGTLHTSSAPKTIDRI
IDAFPPAQQAQIRTQLSEALEAVITQTLLKKKTGGRVAALEIMVATTAVRNLIREAKLHQIPGIMQASQKDGMQTMDMAL
IDLAMRGVVTKAEAQSRSMTPNLFGAPMSGAA

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1115032 NITINOP_RS12160 WP_062486236.1 2582048..2583106(-) (pilT) [Candidatus Nitrospira inopinata isolate ENR4]
ATGGATATTTCCAAATTGTTGACGTTCGCCGTGAAAGAAGGGGCTTCGGATTGCCATCTCAGCGCCGGAGAGCCGCCGAT
GGTCCGCATCCACGGCGACATGAAAAAACTCGATCATCCGGCCCTGACGCCGGAGGAAACCCATGCGCTGATCTACGACA
TGATGAACGATGTGCAGAGGAAGCACTTCGAAGAGCATCGCGAATGCGACTTCTCTTTCGAGTTGGGCGACATCGCACGG
TTCCGCGTGAACGTCTTCGTGCAGCAACGGGGACTCGGCGCGGTGTTCCGAAACATTCCCACCATCATCGTTCCGCTGGA
CAAGCTCGGCATGCCGCCGGTCATTCGGCAGTTGTGCGACAAGGAAAAGGGATTGATCCTGGTCACCGGTCCCACCGGCT
CCGGTAAATCCACCACGCTCGCGGCGATGGTCGATTATCTGAACACGACGTTCGAGGGCCATATCATCACGATCGAAGAT
CCGATCGAGTTCGTGCACAAATCCAAAAAATGCCTCGTCAATCAGCGCGAGCTCGGCGTGCATACCTTGTCCTTCGCCAA
CGCGCTTCGATCGGCCCTGCGCGAGGACCCGGACATCATCCTCGTCGGCGAAATGCGGGATCTCGAAACGATTCAACTGG
CCTTGACCGCCGCGGAAACCGGTCACCTGGTTTTCGGCACGCTGCATACGTCCAGCGCCCCCAAGACCATCGACCGCATC
ATCGATGCGTTTCCGCCGGCCCAACAGGCGCAAATCCGAACGCAATTGTCCGAGGCGTTGGAGGCCGTCATCACGCAGAC
CCTCCTTAAGAAAAAGACCGGCGGCCGAGTCGCCGCGCTCGAGATCATGGTGGCGACCACGGCCGTGCGCAATCTCATCC
GCGAGGCCAAGCTCCATCAAATTCCCGGCATCATGCAGGCCAGCCAAAAAGACGGCATGCAAACGATGGACATGGCGTTG
ATCGACCTCGCGATGCGCGGCGTCGTGACGAAGGCGGAAGCGCAATCCCGCAGCATGACGCCCAATCTCTTCGGCGCGCC
CATGAGCGGGGCCGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S4KW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

61.919

97.727

0.605
  pilT Pseudomonas stutzeri DSM 10701

61.919

97.727

0.605
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.315

95.739

0.597
  pilT Vibrio cholerae strain A1552

62.315

95.739

0.597
  pilT Acinetobacter baylyi ADP1

60.174

97.727

0.588
  pilT Acinetobacter baumannii strain A118

59.302

97.727

0.58
  pilT Acinetobacter baumannii D1279779

59.302

97.727

0.58
  pilT Acinetobacter nosocomialis M2

59.302

97.727

0.58
  pilT Legionella pneumophila strain ERS1305867

59.012

97.727

0.577
  pilT Legionella pneumophila strain Lp02

59.012

97.727

0.577
  pilT Neisseria meningitidis 8013

57.817

96.307

0.557
  pilT Neisseria gonorrhoeae MS11

57.522

96.307

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

98.011

0.523
  pilU Pseudomonas stutzeri DSM 10701

42.09

95.17

0.401
  pilU Acinetobacter baylyi ADP1

40.407

97.727

0.395
  pilU Vibrio cholerae strain A1552

40.233

97.443

0.392

Multiple sequence alignment