Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NITINOP_RS12155 Genome accession   NZ_LN885086
Coordinates   2580843..2582018 (-) Length   391 a.a.
NCBI ID   WP_062486233.1    Uniprot ID   A0A0S4KWL0
Organism   Candidatus Nitrospira inopinata isolate ENR4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2575843..2587018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NITINOP_RS12130 (NITINOP_2630) pyk 2576296..2577750 (-) 1455 WP_062486221.1 pyruvate kinase -
  NITINOP_RS12135 (NITINOP_2631) - 2577859..2578593 (-) 735 WP_062488030.1 intradiol ring-cleavage dioxygenase -
  NITINOP_RS12140 (NITINOP_2632) - 2578599..2579282 (-) 684 WP_062486224.1 Slp family lipoprotein -
  NITINOP_RS12145 (NITINOP_2634) - 2579298..2579921 (-) 624 WP_062486227.1 outer membrane protein -
  NITINOP_RS12150 (NITINOP_2635) - 2580318..2580794 (+) 477 WP_158023403.1 hypothetical protein -
  NITINOP_RS12155 (NITINOP_2636) pilU 2580843..2582018 (-) 1176 WP_062486233.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NITINOP_RS12160 (NITINOP_2637) pilT 2582048..2583106 (-) 1059 WP_062486236.1 type IV pilus twitching motility protein PilT Machinery gene
  NITINOP_RS12165 (NITINOP_2638) bioF 2583228..2584400 (-) 1173 WP_062486239.1 8-amino-7-oxononanoate synthase -
  NITINOP_RS12170 (NITINOP_2639) - 2584525..2585271 (+) 747 WP_062486242.1 tetratricopeptide repeat protein -
  NITINOP_RS12175 (NITINOP_2640) - 2585343..2586650 (-) 1308 WP_062486245.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 43917.47 Da        Isoelectric Point: 7.0753

>NTDB_id=1115031 NITINOP_RS12155 WP_062486233.1 2580843..2582018(-) (pilU) [Candidatus Nitrospira inopinata isolate ENR4]
MDVRSMLKVMVDHEASDLYLTVDAPPIYRVHGSTQPADAPPFTNDQLEALALALMRGQQRGEFEEKMEMNLALYYKELGR
FRVNVFRQRGNVGIVFRHIKAEIQTVEELQLPPIIKDIAMTKRGLVLVVGATGSGKSTTLAAMIDHRNTVQAGHIVTVED
PIEFVHQHKKSIVTQREVGFDTLNFQNALKNALRQAPDVILIGEVRDTETMEAAITFAETGHLCLATLHSNNANQAIERI
MNFFPQERHAQIYLQLSLNLRAIISQRLIPSLDGRRVPALEIMLDTPRVKDLIKKAEVDTLKEAMEQGHEEGCQTFDRVL
FQLYKDNRISLEQALINADSANNLRLKIKLEGLKGDDAVNVLLDRQTGGGTGDAFRIQGGRTDRITPLRKR

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=1115031 NITINOP_RS12155 WP_062486233.1 2580843..2582018(-) (pilU) [Candidatus Nitrospira inopinata isolate ENR4]
ATGGACGTTCGCAGCATGCTCAAGGTCATGGTGGACCATGAAGCGTCCGACCTCTATTTGACCGTGGATGCGCCGCCGAT
TTATCGCGTGCACGGTTCGACGCAACCGGCCGACGCGCCGCCGTTCACCAACGACCAATTGGAAGCGTTGGCCTTGGCGT
TGATGCGCGGTCAACAGCGCGGCGAATTCGAAGAAAAAATGGAAATGAACCTGGCGCTCTACTACAAAGAGCTCGGCCGG
TTCCGCGTCAACGTCTTTCGCCAGCGGGGGAACGTGGGCATCGTGTTCCGCCACATCAAAGCCGAAATCCAAACGGTCGA
GGAGTTGCAGCTCCCTCCGATCATCAAAGACATCGCCATGACTAAACGCGGACTGGTGCTCGTCGTCGGGGCCACCGGCT
CCGGTAAATCCACGACCTTGGCCGCCATGATCGACCATCGCAACACGGTCCAGGCCGGCCACATCGTCACGGTCGAAGAC
CCGATCGAATTCGTGCATCAACACAAAAAATCCATCGTCACGCAGCGCGAGGTGGGCTTCGACACCCTGAATTTCCAAAA
CGCGCTGAAGAACGCTTTGCGCCAAGCTCCCGACGTCATTCTGATCGGCGAGGTGCGCGACACCGAGACGATGGAGGCCG
CCATCACGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCGAATCAGGCGATCGAACGCATC
ATGAATTTTTTTCCGCAGGAGCGGCACGCGCAGATCTATCTGCAACTGTCCCTCAACCTCCGCGCCATCATCTCCCAACG
GTTGATTCCGTCGCTCGACGGGCGGCGCGTACCGGCGTTGGAAATCATGTTGGACACGCCGCGCGTGAAGGATCTGATCA
AGAAGGCCGAAGTCGACACGCTCAAAGAGGCGATGGAACAGGGGCACGAAGAAGGATGCCAGACGTTCGATCGCGTGCTG
TTCCAGTTATACAAGGACAATCGCATCAGCCTGGAACAGGCCTTGATCAACGCCGACAGCGCCAACAATCTCCGGCTGAA
GATCAAACTGGAAGGCTTGAAGGGAGACGACGCCGTCAACGTCTTGCTGGACAGGCAAACCGGCGGAGGAACGGGCGACG
CGTTCAGGATTCAGGGGGGGCGAACCGACAGGATCACTCCCTTGCGGAAACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S4KWL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

52.793

91.56

0.483
  pilU Pseudomonas stutzeri DSM 10701

52.991

89.77

0.476
  pilU Acinetobacter baylyi ADP1

50.852

90.026

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.429

85.934

0.399
  pilT Acinetobacter baylyi ADP1

44.186

87.98

0.389
  pilT Pseudomonas aeruginosa PAK

44.91

85.422

0.384
  pilT Legionella pneumophila strain Lp02

44.91

85.422

0.384
  pilT Legionella pneumophila strain ERS1305867

44.91

85.422

0.384
  pilT Pseudomonas stutzeri DSM 10701

43.402

87.212

0.379
  pilT Acinetobacter baumannii strain A118

43.713

85.422

0.373
  pilT Acinetobacter nosocomialis M2

43.713

85.422

0.373
  pilT Acinetobacter baumannii D1279779

43.713

85.422

0.373
  pilT Vibrio cholerae strain A1552

44.582

82.609

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.582

82.609

0.368

Multiple sequence alignment