Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BN1079_RS10115 Genome accession   NZ_CCSF01000001
Coordinates   2194926..2195960 (-) Length   344 a.a.
NCBI ID   WP_037024107.1    Uniprot ID   A0A078LWS9
Organism   Pseudomonas saudiphocaensis strain 20_BN     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2189926..2200960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN1079_RS10095 (BN1079_02078) - 2191011..2191409 (-) 399 WP_037024098.1 NINE protein -
  BN1079_RS10100 (BN1079_02079) - 2191554..2192765 (+) 1212 WP_037026766.1 putative DNA modification/repair radical SAM protein -
  BN1079_RS10105 (BN1079_02080) - 2192765..2193610 (+) 846 WP_037024099.1 TIGR03915 family putative DNA repair protein -
  BN1079_RS10110 (BN1079_02081) pilU 2193618..2194763 (-) 1146 WP_037024105.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BN1079_RS10115 (BN1079_02082) pilT 2194926..2195960 (-) 1035 WP_037024107.1 type IV pilus twitching motility protein PilT Machinery gene
  BN1079_RS10120 (BN1079_02083) - 2196027..2196752 (+) 726 WP_037024108.1 YggS family pyridoxal phosphate-dependent enzyme -
  BN1079_RS10125 (BN1079_02084) proC 2196749..2197573 (+) 825 WP_037024110.1 pyrroline-5-carboxylate reductase -
  BN1079_RS10130 (BN1079_02085) - 2197583..2198173 (+) 591 WP_037024111.1 YggT family protein -
  BN1079_RS10135 (BN1079_02086) - 2198278..2200227 (+) 1950 WP_037024112.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38070.85 Da        Isoelectric Point: 6.7717

>NTDB_id=111348 BN1079_RS10115 WP_037024107.1 2194926..2195960(-) (pilT) [Pseudomonas saudiphocaensis strain 20_BN]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGCGAVFRTIPSKVLTMEDLGMGEVFRRITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYQHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLMKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KNLLAKGVISRESAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=111348 BN1079_RS10115 WP_037024107.1 2194926..2195960(-) (pilT) [Pseudomonas saudiphocaensis strain 20_BN]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCATCGGATTTGCACCTTTCTTCCGGTCTGCCGCCGAT
GATTCGCGTTGATGGCGATGTACGCCGCATCAATCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTTCGAAGAATTCCTCGAGACCGACTTCTCCTTTGAAGTGCCGGGTGTGGCGCGC
TTCCGGGTTAACGCCTTCAACCAGAACCGCGGTTGCGGTGCGGTGTTCCGGACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTCGGCATGGGTGAGGTCTTCCGCAGGATCACCGACGTGCCGCGCGGCCTGGTACTGGTTACCGGCCCCACCGGCT
CGGGTAAGTCCACGACCCTTGCGGCCATGCTCGACTACCTGAACAACACCAAGTACCAACACATCCTCACCATCGAGGAC
CCGATCGAATTCGTTCACGAATCGAAGAAGTGTCTGGTCAACCAGCGCGAAGTGCACCGCGATACCCTGGGCTTCTCCGA
AGCCTTGCGTTCAGCTCTGCGTGAAGACCCGGACATCATCCTGGTCGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGGCACCTGGTGTTCGGCACGTTGCACACTACCTCGGCCGCCAAGACCATCGACCGGGTG
GTGGATGTCTTCCCGGCCGAGGAAAAGTCCATGGTCCGCTCCATGCTTTCCGAATCGCTGCAGGCGGTAATTTCTCAGAC
TCTGATGAAGAAGATTGGCGGCGGTCGAGTGGCAGCGCACGAGATCATGATCGGCACACCGGCGATTCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGGATGCAGACCCTCGATTCCTGTCTC
AAGAATCTGCTTGCCAAGGGCGTCATTTCCCGTGAAAGCGCCAAGGAGAAAGCCAAGCAACCAGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A078LWS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

97.384

100

0.974
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

82.558

100

0.826
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment