Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BN1079_RS10110 Genome accession   NZ_CCSF01000001
Coordinates   2193618..2194763 (-) Length   381 a.a.
NCBI ID   WP_037024105.1    Uniprot ID   A0A078LY03
Organism   Pseudomonas saudiphocaensis strain 20_BN     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2188618..2199763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN1079_RS10090 (BN1079_02077) - 2189598..2190875 (+) 1278 WP_037024097.1 dihydroorotase -
  BN1079_RS10095 (BN1079_02078) - 2191011..2191409 (-) 399 WP_037024098.1 NINE protein -
  BN1079_RS10100 (BN1079_02079) - 2191554..2192765 (+) 1212 WP_037026766.1 putative DNA modification/repair radical SAM protein -
  BN1079_RS10105 (BN1079_02080) - 2192765..2193610 (+) 846 WP_037024099.1 TIGR03915 family putative DNA repair protein -
  BN1079_RS10110 (BN1079_02081) pilU 2193618..2194763 (-) 1146 WP_037024105.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BN1079_RS10115 (BN1079_02082) pilT 2194926..2195960 (-) 1035 WP_037024107.1 type IV pilus twitching motility protein PilT Machinery gene
  BN1079_RS10120 (BN1079_02083) - 2196027..2196752 (+) 726 WP_037024108.1 YggS family pyridoxal phosphate-dependent enzyme -
  BN1079_RS10125 (BN1079_02084) proC 2196749..2197573 (+) 825 WP_037024110.1 pyrroline-5-carboxylate reductase -
  BN1079_RS10130 (BN1079_02085) - 2197583..2198173 (+) 591 WP_037024111.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42399.56 Da        Isoelectric Point: 6.3485

>NTDB_id=111347 BN1079_RS10110 WP_037024105.1 2193618..2194763(-) (pilU) [Pseudomonas saudiphocaensis strain 20_BN]
MEFEKLLRLMVEKGGSDLFITAGVPPSIKVNGKILPVSKTPMSPEMTRETVHGIMNEQQRREFTENHECNFALSARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIDELKLPGILKDLAMTKRGLVLFVGATGTGKSTSLAAMVGYRNRNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRARETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLGSETDSEHLSSVSQGLSLEVSDDDPGRTFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=111347 BN1079_RS10110 WP_037024105.1 2193618..2194763(-) (pilU) [Pseudomonas saudiphocaensis strain 20_BN]
ATGGAATTCGAAAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGCTCCGACCTGTTCATTACCGCTGGCGTGCCGCCGTC
GATCAAGGTCAACGGCAAGATCCTGCCGGTGAGCAAGACGCCCATGTCGCCGGAAATGACCCGTGAAACCGTGCACGGGA
TCATGAACGAGCAGCAGCGGCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCCTTAGCGCGCGTGGTATCGGCCGC
TTCCGCGTCAGCGCGTTCTACCAGCGCAACCTGGCCGGCATGGTGCTGCGGCGCATCGAAACCAATATCCCCACCATCGA
TGAGCTCAAGCTCCCGGGCATCCTGAAAGATCTGGCCATGACCAAGCGTGGTCTGGTGCTTTTTGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGGTCGGTTACCGCAACAGGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCCATCGAGTTCATCCATCAGCATCAGAATTGCATCGTTACCCAGCGCGAAGTCGGCATCGACACCGACTCGTTCGAGGT
GGCGCTGAAGAACACTCTGCGGCAGGCGCCGGATGTGATCCTGATTGGCGAGATCCGTGCCCGGGAAACCATGGACTACG
CCGTGGCCTTCGCCGAGACCGGGCACCTCTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGCCGACCGCCAGAATCAGGTGTGGATGGATCTGTCGCTGAACCTGAAAGCCATCGTCGCCCAGCA
GCTGATTCCTACCCCTGATGGCAAGGGCCGTCGGGCGGTGATCGAAGTGCTGATCAACACGCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGAGTCTGATGAAGCGTTCTACCGACCTGGGTATGCAGACGTTCGACCAGGCG
CTGTACAACCTCTACGTTCAGGGTGAAATCACTTACGAGGACGCCCTGCTGCATGCCGACTCATCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACAGTGAGCATCTGAGCAGCGTGTCCCAAGGGCTGTCCCTGGAAGTCAGCGACG
ACGATCCGGGGCGGACCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A078LY03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

94.226

100

0.942
  pilU Acinetobacter baylyi ADP1

66.667

93.701

0.625
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

88.976

0.394
  pilT Legionella pneumophila strain ERS1305867

41.791

87.927

0.367
  pilT Legionella pneumophila strain Lp02

41.791

87.927

0.367
  pilT Vibrio cholerae strain A1552

41.42

88.714

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.42

88.714

0.367
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365
  pilT Neisseria meningitidis 8013

40

90.551

0.362
  pilT Neisseria gonorrhoeae MS11

40

90.551

0.362
  pilT Acinetobacter baumannii strain A118

41.194

87.927

0.362
  pilT Acinetobacter baumannii D1279779

41.194

87.927

0.362
  pilT Acinetobacter nosocomialis M2

41.194

87.927

0.362

Multiple sequence alignment