Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KHN94_RS12280 Genome accession   NZ_HG992758
Coordinates   2701159..2702199 (-) Length   346 a.a.
NCBI ID   WP_182032068.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16 isolate B1REV17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2696159..2707199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN94_RS12250 (ACOMICROBIO_LOCUS1774) endA 2696291..2696986 (+) 696 WP_182032063.1 deoxyribonuclease I -
  KHN94_RS12255 (ACOMICROBIO_LOCUS1775) rsmE 2697110..2697841 (+) 732 WP_182032064.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KHN94_RS12260 (ACOMICROBIO_LOCUS1776) gshB 2697856..2698803 (+) 948 WP_182032065.1 glutathione synthase -
  KHN94_RS12265 (ACOMICROBIO_LOCUS1777) - 2698910..2699473 (+) 564 WP_182032066.1 YqgE/AlgH family protein -
  KHN94_RS12270 (ACOMICROBIO_LOCUS1778) ruvX 2699522..2699947 (+) 426 WP_182032067.1 Holliday junction resolvase RuvX -
  KHN94_RS12275 (ACOMICROBIO_LOCUS1779) pilU 2700023..2701129 (-) 1107 WP_182011695.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHN94_RS12280 (ACOMICROBIO_LOCUS1780) pilT 2701159..2702199 (-) 1041 WP_182032068.1 type IV pilus twitching motility protein PilT Machinery gene
  KHN94_RS12285 (ACOMICROBIO_EPCKBFOG_02510) - 2702227..2702940 (+) 714 WP_182032069.1 YggS family pyridoxal phosphate-dependent enzyme -
  KHN94_RS12290 (ACOMICROBIO_LOCUS1781) proC 2703062..2703880 (+) 819 WP_182032070.1 pyrroline-5-carboxylate reductase -
  KHN94_RS12295 (ACOMICROBIO_EPCKBFOG_02512) - 2703929..2704486 (+) 558 WP_182032071.1 YggT family protein -
  KHN94_RS12300 (ACOMICROBIO_EPCKBFOG_02513) yggU 2704486..2704773 (+) 288 WP_224914019.1 DUF167 family protein YggU -
  KHN94_RS12305 (ACOMICROBIO_EPCKBFOG_02514) - 2704881..2705312 (+) 432 WP_182011690.1 DUF4426 domain-containing protein -
  KHN94_RS12310 (ACOMICROBIO_LOCUS1782) - 2705507..2706109 (+) 603 WP_182032073.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38394.85 Da        Isoelectric Point: 6.6193

>NTDB_id=1112536 KHN94_RS12280 WP_182032068.1 2701159..2702199(-) (pilT) [Vibrio sp. B1FLJ16 isolate B1REV17]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELQNVGR
FRVNAFNQSRGCAAVFRTIPSIIPTLEELDAPDIFRKIANAQKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLLAQGIVSHEEADSKVGLDVQPFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1112536 KHN94_RS12280 WP_182032068.1 2701159..2702199(-) (pilT) [Vibrio sp. B1FLJ16 isolate B1REV17]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAATGCTTCGGATCTACATCTTTCTGCTGGCGTACCGCCTAT
GGTCAGAATCGATGGCGATGTACGAAAGCTAGGTATTCCGGCGTTTACACACCAAGAGGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCTCAACGCAGTGAGTTTGAAGAAAAGCTTGAAGTCGACTTCTCGTTTGAACTCCAGAACGTAGGTCGT
TTTCGTGTTAACGCATTTAACCAATCTCGTGGTTGTGCTGCGGTATTTCGTACTATTCCAAGCATTATACCGACTCTCGA
AGAACTGGATGCGCCGGATATCTTCCGCAAAATTGCCAATGCACAAAAAGGTCTCGTTCTGGTCACAGGGCCAACCGGTT
CTGGTAAATCGACGACCTTAGCTGCCATTGTCGACTACATTAACCGTAATCACAATAAGCACGTTCTCACAATTGAAGAT
CCGATTGAATTTGTCCATACCAACAACAAATGTCTGATCAACCAACGTGAAGTTCACCGTGATACGCATAGCTTTAAAAA
TGCTTTACGTAGTGCGCTGCGTGAAGACCCAGACGTCATCTTGGTCGGTGAGATGCGTGATAAAGAGACTATCAGTCTTG
CGTTAACCGCTGCGGAAACGGGTCACTTAGTGTTTGGTACTCTGCACACGAGTTCTGCTGCGAAAACCATTGACCGTATT
ATTGATGTGTTCCCAGGCAGCGATAAAGACATGGTTCGTTCAATGCTATCTGAATCTTTACGTTCTGTTATTGCCCAGAA
GCTGCTGAAACGTAATGGTGGTGGAAGAGTAGCTTGTCATGAAATCATGATGGCGACTCCGGCAATCCGTAACTTAATTC
GTGAAGACAAAGTCGCTCAGATGTACTCGATCATTCAGACGGGCGCGGCTCACGGAATGCAGACTATGGAGCAAAACGCT
CGACAGTTGCTCGCTCAGGGTATTGTTTCTCATGAAGAGGCTGACAGCAAAGTCGGTCTTGATGTCCAACCGTTCTCGTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

71.856

96.532

0.694
  pilT Acinetobacter baumannii strain A118

71.856

96.532

0.694
  pilT Acinetobacter nosocomialis M2

71.557

96.532

0.691
  pilT Acinetobacter baylyi ADP1

72.424

95.376

0.691
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.399

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.449

98.555

0.665
  pilT Legionella pneumophila strain ERS1305867

67.449

98.555

0.665
  pilT Neisseria meningitidis 8013

65.672

96.821

0.636
  pilT Neisseria gonorrhoeae MS11

65.373

96.821

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.746

96.821

0.491
  pilU Vibrio cholerae strain A1552

41.493

96.821

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.821

0.396
  pilU Acinetobacter baylyi ADP1

37.798

97.11

0.367

Multiple sequence alignment