Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KHN94_RS12275 Genome accession   NZ_HG992758
Coordinates   2700023..2701129 (-) Length   368 a.a.
NCBI ID   WP_182011695.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16 isolate B1REV17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2695023..2706129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN94_RS12245 (ACOMICROBIO_EPCKBFOG_02502) - 2695656..2696153 (+) 498 WP_182032062.1 SprT family zinc-dependent metalloprotease -
  KHN94_RS12250 (ACOMICROBIO_LOCUS1774) endA 2696291..2696986 (+) 696 WP_182032063.1 deoxyribonuclease I -
  KHN94_RS12255 (ACOMICROBIO_LOCUS1775) rsmE 2697110..2697841 (+) 732 WP_182032064.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KHN94_RS12260 (ACOMICROBIO_LOCUS1776) gshB 2697856..2698803 (+) 948 WP_182032065.1 glutathione synthase -
  KHN94_RS12265 (ACOMICROBIO_LOCUS1777) - 2698910..2699473 (+) 564 WP_182032066.1 YqgE/AlgH family protein -
  KHN94_RS12270 (ACOMICROBIO_LOCUS1778) ruvX 2699522..2699947 (+) 426 WP_182032067.1 Holliday junction resolvase RuvX -
  KHN94_RS12275 (ACOMICROBIO_LOCUS1779) pilU 2700023..2701129 (-) 1107 WP_182011695.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHN94_RS12280 (ACOMICROBIO_LOCUS1780) pilT 2701159..2702199 (-) 1041 WP_182032068.1 type IV pilus twitching motility protein PilT Machinery gene
  KHN94_RS12285 (ACOMICROBIO_EPCKBFOG_02510) - 2702227..2702940 (+) 714 WP_182032069.1 YggS family pyridoxal phosphate-dependent enzyme -
  KHN94_RS12290 (ACOMICROBIO_LOCUS1781) proC 2703062..2703880 (+) 819 WP_182032070.1 pyrroline-5-carboxylate reductase -
  KHN94_RS12295 (ACOMICROBIO_EPCKBFOG_02512) - 2703929..2704486 (+) 558 WP_182032071.1 YggT family protein -
  KHN94_RS12300 (ACOMICROBIO_EPCKBFOG_02513) yggU 2704486..2704773 (+) 288 WP_224914019.1 DUF167 family protein YggU -
  KHN94_RS12305 (ACOMICROBIO_EPCKBFOG_02514) - 2704881..2705312 (+) 432 WP_182011690.1 DUF4426 domain-containing protein -
  KHN94_RS12310 (ACOMICROBIO_LOCUS1782) - 2705507..2706109 (+) 603 WP_182032073.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41327.50 Da        Isoelectric Point: 6.5951

>NTDB_id=1112535 KHN94_RS12275 WP_182011695.1 2700023..2701129(-) (pilU) [Vibrio sp. B1FLJ16 isolate B1REV17]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVAQLLDNAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETEIPSFEQLKLPVVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNRAGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKSGKGRHGVFEVLLNSPRISDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMEGKISEEDALHSADSANDLRLMLKTQRGEPLATGSLANVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1112535 KHN94_RS12275 WP_182011695.1 2700023..2701129(-) (pilU) [Vibrio sp. B1FLJ16 isolate B1REV17]
ATGGATTTGAATAAATTTCTTGAAGGTATGTTGGCGCTTAAAGCGTCAGACCTTTATATCACAGTGGGTGCCCCCATCCT
GTTCCGGGTGGATGGTGAACTGCGCCCGCAAGGCGATAAACTGACTGAAAATGACGTGGCTCAGCTGCTGGATAACGCAA
TGGATCCTGAGCGTCGTCAGGAGTTTCGTAAAAGTCGTGAGTCTAACTTCGCTATTGTCAGAGATTGCGGCCGTTTCCGT
GTTAGTGCTTTTTTCCAGCGCGAGCTGCCAGGTGCTGTCATCCGCCGAATCGAGACTGAAATTCCGAGTTTTGAGCAGCT
TAAACTGCCTGTTGTATTGCAGGATCTGGCGATTGCCAAGCGCGGGTTAGTACTGGTGGTCGGTGCAACGGGCTCGGGTA
AGTCTACAACAATGGCGGCGATGACGGGCTACCGAAACAGTAACCGAGCCGGACATATTCTGACGGTAGAAGACCCGATT
GAGTTCGTGCATGAACACAAGCGTTGTATCGTGACGCAACGTGAAGTGGGGTTAGATACTGAAAGTTATGAAATCGCACT
GAAAAACTCGTTGCGTCAGGCTCCGGATATGATCCTGATTGGTGAGATCCGTAGCCGGGAGACAATGGAATACGCGATGA
CTTTCGCAGAAACAGGCCACTTGTGTATGGCGACGTTGCACGCCAATAATGCCAACCAGGCTCTGGAACGCATTCTTCAT
TTAGTGCCGAAAGATCAAAAAGAGCAGTTCCTGTTTGATCTCTCAATGAACCTGAAGGGAGTAATCGGTCAGCAGCTGAT
CCGCGATAAAAGCGGCAAAGGGCGTCATGGCGTGTTTGAGGTATTGCTGAACAGCCCGCGTATTTCGGATTTGATCCGAC
GAGGCGATTTGCATGAGCTGAAATCGACCATGGCGCGTTCAAACGAGTTTGGCATGCTGACCTTCGACCAGTCACTTTAC
AAACTGGTGATGGAAGGGAAAATCAGCGAAGAGGATGCATTGCACAGTGCAGATTCTGCAAATGACCTACGTCTGATGCT
GAAAACCCAGCGTGGTGAACCACTTGCGACGGGAAGTTTGGCCAACGTTAAGATCGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

56.125

95.38

0.535
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

41.916

90.761

0.38
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.769

88.315

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372

Multiple sequence alignment