Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KJB05_RS02780 Genome accession   NZ_HG992742
Coordinates   579768..580808 (+) Length   346 a.a.
NCBI ID   WP_005436511.1    Uniprot ID   A0A0C1VE31
Organism   Vibrio sp. B1ASS3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 574768..585808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJB05_RS02745 (ACOMICROBIO_LOCUS365) hemW 574792..575964 (-) 1173 WP_208445665.1 radical SAM family heme chaperone HemW -
  KJB05_RS02750 (ACOMICROBIO_LOCUS366) - 575983..576585 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  KJB05_RS02755 (ACOMICROBIO_NCLOACGD_00560) - 576689..577120 (-) 432 WP_208445666.1 DUF4426 domain-containing protein -
  KJB05_RS02760 (ACOMICROBIO_NCLOACGD_00561) yggU 577180..577470 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  KJB05_RS02765 (ACOMICROBIO_NCLOACGD_00562) - 577470..578027 (-) 558 WP_005425806.1 YggT family protein -
  KJB05_RS02770 (ACOMICROBIO_LOCUS367) proC 578081..578899 (-) 819 WP_208445667.1 pyrroline-5-carboxylate reductase -
  KJB05_RS02775 (ACOMICROBIO_NCLOACGD_00564) - 579030..579739 (-) 710 Protein_476 YggS family pyridoxal phosphate-dependent enzyme -
  KJB05_RS02780 (ACOMICROBIO_LOCUS368) pilT 579768..580808 (+) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  KJB05_RS02785 (ACOMICROBIO_LOCUS369) pilU 580840..581946 (+) 1107 WP_005436509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KJB05_RS02790 (ACOMICROBIO_LOCUS370) ruvX 582008..582433 (-) 426 WP_005436506.1 Holliday junction resolvase RuvX -
  KJB05_RS02795 (ACOMICROBIO_LOCUS371) - 582485..583048 (-) 564 WP_005436504.1 YqgE/AlgH family protein -
  KJB05_RS02800 (ACOMICROBIO_LOCUS372) gshB 583177..584127 (-) 951 WP_208445669.1 glutathione synthase -
  KJB05_RS02805 (ACOMICROBIO_LOCUS373) rsmE 584142..584873 (-) 732 WP_005436502.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KJB05_RS02810 (ACOMICROBIO_LOCUS374) endA 585020..585715 (-) 696 WP_020197218.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38661.14 Da        Isoelectric Point: 6.5043

>NTDB_id=1112380 KJB05_RS02780 WP_005436511.1 579768..580808(+) (pilT) [Vibrio sp. B1ASS3]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1112380 KJB05_RS02780 WP_005436511.1 579768..580808(+) (pilT) [Vibrio sp. B1ASS3]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAGCTGGGAATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGCAGTATTCCAACACTGGA
AGAGCTAGAAGCTCCTGAGATCTTCAGAAAAATCGCTAACGCCGAGAAAGGTCTGGTACTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACGACCTTGGCAGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACATCCTAACAATTGAAGAT
CCAATTGAATTTGTTCACAACAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTGCGTAGTGCATTGCGTGAAGACCCGGATGTGATTCTAGTTGGTGAGATGCGTGACAAAGAAACAATCAGTCTAG
CGCTGACAGCGGCTGAAACCGGTCACCTTGTTTTCGGTACACTACACACGAGCTCCGCGGCGAAAACCATCGACCGTATT
ATCGATGTGTTCCCGGGGAGCGATAAAGACATGGTGCGCTCGATGTTGTCGGAATCTCTCCGTTCGGTTATCGCGCAAAA
GCTTTTGAAACGTAACGGCGGTGGTCGTATCGCGTGTCATGAAATCATGATGGCAACACCTGCGATCCGAAACCTTATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATCATTCAGACCGGTGCAGCTCACGGCATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAGGGCATGGTCTCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1VE31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.424

95.376

0.691
  pilT Acinetobacter baumannii strain A118

72.424

95.376

0.691
  pilT Acinetobacter nosocomialis M2

72.121

95.376

0.688
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.322

97.977

0.679
  pilT Pseudomonas aeruginosa PAK

69.118

98.266

0.679
  pilT Legionella pneumophila strain Lp02

67.976

95.665

0.65
  pilT Legionella pneumophila strain ERS1305867

67.976

95.665

0.65
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment