Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KJB05_RS02785 Genome accession   NZ_HG992742
Coordinates   580840..581946 (+) Length   368 a.a.
NCBI ID   WP_005436509.1    Uniprot ID   A0A812FD73
Organism   Vibrio sp. B1ASS3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575840..586946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJB05_RS02750 (ACOMICROBIO_LOCUS366) - 575983..576585 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  KJB05_RS02755 (ACOMICROBIO_NCLOACGD_00560) - 576689..577120 (-) 432 WP_208445666.1 DUF4426 domain-containing protein -
  KJB05_RS02760 (ACOMICROBIO_NCLOACGD_00561) yggU 577180..577470 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  KJB05_RS02765 (ACOMICROBIO_NCLOACGD_00562) - 577470..578027 (-) 558 WP_005425806.1 YggT family protein -
  KJB05_RS02770 (ACOMICROBIO_LOCUS367) proC 578081..578899 (-) 819 WP_208445667.1 pyrroline-5-carboxylate reductase -
  KJB05_RS02775 (ACOMICROBIO_NCLOACGD_00564) - 579030..579739 (-) 710 Protein_476 YggS family pyridoxal phosphate-dependent enzyme -
  KJB05_RS02780 (ACOMICROBIO_LOCUS368) pilT 579768..580808 (+) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  KJB05_RS02785 (ACOMICROBIO_LOCUS369) pilU 580840..581946 (+) 1107 WP_005436509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KJB05_RS02790 (ACOMICROBIO_LOCUS370) ruvX 582008..582433 (-) 426 WP_005436506.1 Holliday junction resolvase RuvX -
  KJB05_RS02795 (ACOMICROBIO_LOCUS371) - 582485..583048 (-) 564 WP_005436504.1 YqgE/AlgH family protein -
  KJB05_RS02800 (ACOMICROBIO_LOCUS372) gshB 583177..584127 (-) 951 WP_208445669.1 glutathione synthase -
  KJB05_RS02805 (ACOMICROBIO_LOCUS373) rsmE 584142..584873 (-) 732 WP_005436502.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KJB05_RS02810 (ACOMICROBIO_LOCUS374) endA 585020..585715 (-) 696 WP_020197218.1 deoxyribonuclease I -
  KJB05_RS02815 (ACOMICROBIO_NCLOACGD_00573) - 585866..586363 (-) 498 WP_005436498.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41281.39 Da        Isoelectric Point: 6.5939

>NTDB_id=1112381 KJB05_RS02785 WP_005436509.1 580840..581946(+) (pilU) [Vibrio sp. B1ASS3]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVAVLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEELKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKTTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEGFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1112381 KJB05_RS02785 WP_005436509.1 580840..581946(+) (pilU) [Vibrio sp. B1ASS3]
ATGGATTTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTTGGTGCGCCGATCTT
GTTTCGTGTCGATGGCGAGCTGCGCCCGCAAGGCGATAAGTTGACTGAAAATGATGTTGCTGTGCTACTCGATAGTGCGA
TGGATCCGGAGCGACGTCAGGAGTTTCGTAAAAGCCGTGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGCGCCTTCTTCCAACGTGAATTGCCTGGCGCGGTGATTCGTCGTATCGAGACCAATATTCCTACTTTTGAAGAACT
TAAACTCCCTTTGGTTCTACAAGACCTTGCGATTGCAAAGCGTGGTTTGGTGCTGGTGGTTGGTGCAACAGGTTCTGGTA
AATCAACGACTATGGCCGCGATGACTGGCTACCGTAACAGCAATAAAACGGGGCATATTCTTACGGTAGAAGACCCGATT
GAATTTGTGCACGAGCATAAGCGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACCGACAGCTATGAAGTAGCGTT
GAAGAACTCATTACGTCAAGCACCAGACATGATTTTGATTGGTGAGATTCGTAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAGACGGGCCACTTGTGTATGGCGACACTGCACGCCAACAATGCAAACCAAGCGCTTGAGCGTATTCTGCAC
TTGGTGCCGAAAGATCAGAAAGATCAGTTCCTGTTTGATCTGTCGATGAACTTAAAAGGCGTGGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAAGGTCGTCATGGTGTGTTTGAGATCTTGTTAAACAGTCCACGCGTTTCGGATCTGATCCGCC
GTGGTGATCTGCATGAGTTGAAAACGACCATGGCACGCTCGAATGAATTTGGGATGCTGACCTTTGACCAGTCACTCTAC
AAGCTAGTGATGCAAGGTAAGATCAGTGAAGAGGATGCTTTGCACAGTGCTGATTCAGCTAACGATCTGCGTTTGATGTT
GAAGACGCAGCGCGGTGAAGGCTTCTCAACGGGTACGCTGGCGAATGTAAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A812FD73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.793

100

0.818
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment