Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BN5_RS19365 Genome accession   NZ_HG916826
Coordinates   4086823..4087857 (-) Length   344 a.a.
NCBI ID   WP_003463935.1    Uniprot ID   A0A061CZ29
Organism   Ectopseudomonas oleovorans CECT 5344 strain CECT5344     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4081823..4092857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN5_RS19345 (BN5_3963) - 4082902..4083300 (-) 399 WP_021490398.1 TM2 domain-containing protein -
  BN5_RS19350 (BN5_3964) - 4083448..4084659 (+) 1212 WP_003463930.1 putative DNA modification/repair radical SAM protein -
  BN5_RS19355 (BN5_3965) - 4084659..4085546 (+) 888 WP_003463932.1 TIGR03915 family putative DNA repair protein -
  BN5_RS19360 (BN5_3966) pilU 4085512..4086657 (-) 1146 WP_003463934.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BN5_RS19365 (BN5_3967) pilT 4086823..4087857 (-) 1035 WP_003463935.1 type IV pilus twitching motility protein PilT Machinery gene
  BN5_RS19370 (BN5_3968) - 4087933..4088625 (+) 693 WP_003463937.1 YggS family pyridoxal phosphate-dependent enzyme -
  BN5_RS19375 (BN5_3969) proC 4088643..4089461 (+) 819 WP_003463939.1 pyrroline-5-carboxylate reductase -
  BN5_RS19380 (BN5_3970) - 4089473..4090066 (+) 594 WP_003463941.1 YggT family protein -
  BN5_RS19385 (BN5_3971) - 4090148..4090453 (+) 306 WP_039965285.1 DUF167 domain-containing protein -
  BN5_RS19390 (BN5_3972) - 4090630..4092606 (+) 1977 WP_003463944.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37933.62 Da        Isoelectric Point: 6.8890

>NTDB_id=1112130 BN5_RS19365 WP_003463935.1 4086823..4087857(-) (pilT) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSKGLVSRDSAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1112130 BN5_RS19365 WP_003463935.1 4086823..4087857(-) (pilT) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGGATATCACTGAGCTGCTCGCCTTCAGCGCCAAGCAAGGCGCGTCGGATTTGCACCTCTCGGCGGGACTGCCGCCGAT
GATCCGCGTCGACGGCGATGTGCGCCGTATCAACCTGCCGCCGCTGGATCACAAGCAGGTACACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGACTTCGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCGGGTGTGGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGTGCTGTGTTCCGTACCATTCCATCCAAGGTACTGACCATGGA
AGACCTCGGCATGGGCGAGGTGTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CGGGTAAGTCGACCACCTTGGCGGCGATGCTCGACTACCTCAACAGCAACAAGTACCACCATATCCTCACCATCGAAGAC
CCAATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCGCTGCGTTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACTTCTGCTGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAGAAGTCGATGGTGCGTTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCACAGAC
CCTGCTGAAGAAGATCGGTGGCGGTCGCGTGGCCGCGCACGAAATCATGATCGGTACGCCTGCCATTCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGTGCGCTGGGCATGCAGACCCTCGATGCGTGCCTC
AAGACCCTGGTATCCAAGGGCCTGGTCAGCCGCGACAGTGCGCGCGAGAAGGCCAAGAGCCCGGAAAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A061CZ29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.349

100

0.953
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment