Detailed information NTG1112129

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BN5_RS19360
• Genome accession: NZ_HG916826
• Coordinates: 4085512..4086657 (-)
• Length: 381 a.a.
• NCBI ID: WP_003463934.1
• Uniprot ID: W6R2Q0
• Organism: Ectopseudomonas oleovorans CECT 5344 strain CECT5344
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4080512..4091657

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BN5_RS19340 (BN5_3962)	ntrC	4081159..4082592 (+)	1434	WP_003463926.1	nitrogen regulation protein NR(I)	-
BN5_RS19345 (BN5_3963)	-	4082902..4083300 (-)	399	WP_021490398.1	TM2 domain-containing protein	-
BN5_RS19350 (BN5_3964)	-	4083448..4084659 (+)	1212	WP_003463930.1	putative DNA modification/repair radical SAM protein	-
BN5_RS19355 (BN5_3965)	-	4084659..4085546 (+)	888	WP_003463932.1	TIGR03915 family putative DNA repair protein	-
BN5_RS19360 (BN5_3966)	pilU	4085512..4086657 (-)	1146	WP_003463934.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BN5_RS19365 (BN5_3967)	pilT	4086823..4087857 (-)	1035	WP_003463935.1	type IV pilus twitching motility protein PilT	Machinery gene
BN5_RS19370 (BN5_3968)	-	4087933..4088625 (+)	693	WP_003463937.1	YggS family pyridoxal phosphate-dependent enzyme	-
BN5_RS19375 (BN5_3969)	proC	4088643..4089461 (+)	819	WP_003463939.1	pyrroline-5-carboxylate reductase	-
BN5_RS19380 (BN5_3970)	-	4089473..4090066 (+)	594	WP_003463941.1	YggT family protein	-
BN5_RS19385 (BN5_3971)	-	4090148..4090453 (+)	306	WP_039965285.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42477.88 Da
• Isoelectric Point: 6.7729

>NTDB_id=1112129 BN5_RS19360 WP_003463934.1 4085512..4086657(-) (pilU) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEMTRETVLGVMNEAQRREFAEKHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1112129 BN5_RS19360 WP_003463934.1 4085512..4086657(-) (pilU) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGGAATTCGAAAAATTACTGCGCCTGATGGTCGAAAAAGGCGGTTCCGACCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCGCCGGAGATGACCCGCGAAACCGTGCTGGGCG
TGATGAACGAGGCGCAGCGCCGCGAGTTTGCCGAAAAGCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGTCGC
TTCCGTGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATTCCCACCATCGA
CGACCTCAAGCTGCCGGAAATTCTCAAGAAACTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGTAAGTCCACTTCGCTGGCGGCAATGATCGGCTACCGCAACAGGAACAGCAGCGGTCATATCATCTCCATCGAAGAT
CCGATCGAGTACATCCACCAGCACCAGAGCTGCATCGTCACTCAGCGTGAAGTGGGTATCGACACCGAGTCGTTCGAAGT
GGCGCTGAAGAACACGCTGCGTCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGCACCCGCGAGACCATGGATCATG
CCGTGGCTTTCGCCGAAACCGGTCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATTGTCGCTCAGCA
GCTGATTCCAACCCCAGATGGTAAAGGTCGCCGCGCGGTGATCGAGGTCCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAAGATGCGTTGCTCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGATGGCGATCACCTGACCAGCATGGCTCAGGGGTTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

3D structure

• AlphaFold DB: W6R2Q0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.126	100	0.921
pilU	Acinetobacter baylyi ADP1	64.986	93.701	0.609
pilU	Vibrio cholerae strain A1552	59.143	91.864	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386