Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOI0R_RS07615 Genome accession   NZ_CP184802
Coordinates   447809..448843 (+) Length   344 a.a.
NCBI ID   WP_115425628.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. piscicida strain DQ-SS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442809..453843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOI0R_RS07580 - 443054..443458 (+) 405 WP_050787742.1 DUF2884 family protein -
  ACOI0R_RS07585 hemW 443531..444691 (-) 1161 WP_065170686.1 radical SAM family heme chaperone HemW -
  ACOI0R_RS07590 - 444691..445284 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  ACOI0R_RS07595 yggU 445386..445685 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  ACOI0R_RS07600 - 445682..446239 (-) 558 WP_005297682.1 YggT family protein -
  ACOI0R_RS07605 proC 446251..447069 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  ACOI0R_RS07610 - 447085..447786 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOI0R_RS07615 pilT 447809..448843 (+) 1035 WP_115425628.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOI0R_RS07620 pilU 448855..449961 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOI0R_RS07625 ruvX 450289..450714 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  ACOI0R_RS07630 - 450711..451274 (-) 564 WP_044174430.1 YqgE/AlgH family protein -
  ACOI0R_RS07635 gshB 451428..452375 (-) 948 WP_005297697.1 glutathione synthase -
  ACOI0R_RS07640 rsmE 452396..453127 (-) 732 WP_044174434.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38190.77 Da        Isoelectric Point: 6.1433

>NTDB_id=1111334 ACOI0R_RS07615 WP_115425628.1 447809..448843(+) (pilT) [Photobacterium damselae subsp. piscicida strain DQ-SS1]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHPEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1111334 ACOI0R_RS07615 WP_115425628.1 447809..448843(+) (pilT) [Photobacterium damselae subsp. piscicida strain DQ-SS1]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACCCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGCCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACCTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCGGCATTGGTTGATTATATTAATGAGCACACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTGCATCGTGATACTCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCCGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCGGTAGTTTCCCAAAG
TTTGTTGAAATGTATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACTCCTGCGATTCGAAATTTGATCC
GTGAAGATAAGATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCAATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCCCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATAGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.58

96.802

0.77
  pilT Vibrio cholerae strain A1552

79.58

96.802

0.77
  pilT Acinetobacter baumannii D1279779

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.448

97.384

0.686
  pilT Pseudomonas aeruginosa PAK

70.149

97.384

0.683
  pilT Acinetobacter nosocomialis M2

70.149

97.384

0.683
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.129

99.419

0.677
  pilT Legionella pneumophila strain Lp02

69.605

95.64

0.666
  pilT Legionella pneumophila strain ERS1305867

69.605

95.64

0.666
  pilT Neisseria meningitidis 8013

65.579

97.965

0.642
  pilT Neisseria gonorrhoeae MS11

65.282

97.965

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

99.128

0.497
  pilU Pseudomonas stutzeri DSM 10701

41.329

100

0.416
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366

Multiple sequence alignment