Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACODT9_RS00635 Genome accession   NZ_CP184747
Coordinates   141440..142477 (+) Length   345 a.a.
NCBI ID   WP_014146644.1    Uniprot ID   -
Organism   Methylotuvimicrobium alcaliphilum strain 20ZK     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 136440..147477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACODT9_RS00610 (ACODT9_00610) - 137584..138966 (-) 1383 WP_014146639.1 selenium-binding protein SBP56-related protein -
  ACODT9_RS00615 (ACODT9_00615) - 139068..139343 (-) 276 WP_014146640.1 DUF4242 domain-containing protein -
  ACODT9_RS00620 (ACODT9_00620) - 139395..139991 (-) 597 WP_014146641.1 TetR/AcrR family transcriptional regulator -
  ACODT9_RS00625 (ACODT9_00625) - 140241..140504 (+) 264 WP_014146642.1 hypothetical protein -
  ACODT9_RS00630 (ACODT9_00630) - 140643..141326 (-) 684 WP_014146643.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACODT9_RS00635 (ACODT9_00635) pilT 141440..142477 (+) 1038 WP_014146644.1 type IV pilus twitching motility protein PilT Machinery gene
  ACODT9_RS00640 (ACODT9_00640) pilU 142488..143621 (+) 1134 WP_014146645.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACODT9_RS00645 (ACODT9_00645) djlA 143627..144436 (-) 810 WP_014146646.1 co-chaperone DjlA -
  ACODT9_RS00650 (ACODT9_00650) - 144511..145410 (+) 900 WP_014146647.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  ACODT9_RS00655 (ACODT9_00655) radC 145549..146223 (-) 675 WP_014146648.1 RadC family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38364.27 Da        Isoelectric Point: 6.8404

>NTDB_id=1111101 ACODT9_RS00635 WP_014146644.1 141440..142477(+) (pilT) [Methylotuvimicrobium alcaliphilum strain 20ZK]
MDIAELLTFSVKNNASDLHLSAGLPPMIRVDGDIRRINIPALEHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQERGAGAVFRTIPSKVLSLEDLNAPKFFEEVTRKPRGLILVTGPTGSGKSTTLAAMINHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFTEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKEGMQTLDQNL
KEMVEKGLVASKVAMMKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1111101 ACODT9_RS00635 WP_014146644.1 141440..142477(+) (pilT) [Methylotuvimicrobium alcaliphilum strain 20ZK]
ATGGATATTGCTGAATTATTGACTTTTTCCGTTAAAAACAATGCATCGGATTTACATTTATCGGCCGGTTTGCCGCCGAT
GATTCGAGTGGACGGCGATATCCGGCGTATCAATATTCCTGCCTTGGAGCATAAAGAAGTTCATGCTTTGATTTACGACA
TCATGAACGATAAACAGCGCCGTGATTATGAAGAATTTCTTGAAACCGACTTTTCTTTTGCATTGCCGGGCGTTGCAAGG
TTTCGTGTCAATGCGTTTAATCAGGAACGCGGTGCTGGTGCAGTTTTTAGAACGATTCCTTCGAAGGTGTTGAGTTTAGA
GGATCTGAATGCGCCAAAGTTTTTCGAAGAAGTCACGCGCAAACCGAGAGGTTTGATACTGGTAACAGGACCTACCGGGT
CGGGTAAATCGACGACGCTCGCGGCGATGATCAACCATATCAACACCAATGATTATGCACATATTTTAACCGTTGAGGAC
CCGATCGAGTTCGTACATGAAAGCCAAAAATGCTTGATCAACCAACGCGAAGTCCATCGCGATACTTTAGGGTTCACCGA
AGCGTTGCGTTCCGCATTGCGGGAAGACCCCGATGTTATTCTAGTGGGCGAAATGCGCGACTTGGAAACGATACGATTGG
CACTGACCGCGGCAGAAACCGGTCACTTGGTTTTCGGCACGTTGCACACCACGTCGGCCGCGAAAACGATCGACCGCATT
ATCGATGTGTTTCCGGCGGCTGAAAAAGACATGATCCGCTCGATGTTGTCGGAATCGTTGCAAGCGGTTATTTCGCAAAC
CTTGCTGAAAAAAGTCGGAGGAGGACGTATCGCGGCGCATGAAATCATGGTCGGGACTGCTGCCATTAGAAATTTGATTC
GCGAAGCGAAAGTTGCGCAAATGTATTCGGCGATACAAACCGGGCGCAAGGAAGGCATGCAGACCTTGGATCAAAATTTG
AAAGAGATGGTTGAAAAAGGGTTAGTGGCTTCAAAAGTCGCCATGATGAAAGCCGTTAACAAAGACATGTTCAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.882

97.971

0.783
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.482

96.232

0.678
  pilT Vibrio cholerae strain A1552

70.482

96.232

0.678
  pilT Neisseria meningitidis 8013

66.764

99.42

0.664
  pilT Neisseria gonorrhoeae MS11

66.181

99.42

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Acinetobacter baylyi ADP1

39.769

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.017

100

0.391
  pilU Vibrio cholerae strain A1552

38.15

100

0.383

Multiple sequence alignment