Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACODT9_RS00640 Genome accession   NZ_CP184747
Coordinates   142488..143621 (+) Length   377 a.a.
NCBI ID   WP_014146645.1    Uniprot ID   G4SUK1
Organism   Methylotuvimicrobium alcaliphilum strain 20ZK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137488..148621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACODT9_RS00610 (ACODT9_00610) - 137584..138966 (-) 1383 WP_014146639.1 selenium-binding protein SBP56-related protein -
  ACODT9_RS00615 (ACODT9_00615) - 139068..139343 (-) 276 WP_014146640.1 DUF4242 domain-containing protein -
  ACODT9_RS00620 (ACODT9_00620) - 139395..139991 (-) 597 WP_014146641.1 TetR/AcrR family transcriptional regulator -
  ACODT9_RS00625 (ACODT9_00625) - 140241..140504 (+) 264 WP_014146642.1 hypothetical protein -
  ACODT9_RS00630 (ACODT9_00630) - 140643..141326 (-) 684 WP_014146643.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACODT9_RS00635 (ACODT9_00635) pilT 141440..142477 (+) 1038 WP_014146644.1 type IV pilus twitching motility protein PilT Machinery gene
  ACODT9_RS00640 (ACODT9_00640) pilU 142488..143621 (+) 1134 WP_014146645.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACODT9_RS00645 (ACODT9_00645) djlA 143627..144436 (-) 810 WP_014146646.1 co-chaperone DjlA -
  ACODT9_RS00650 (ACODT9_00650) - 144511..145410 (+) 900 WP_014146647.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  ACODT9_RS00655 (ACODT9_00655) radC 145549..146223 (-) 675 WP_014146648.1 RadC family protein -
  ACODT9_RS00660 (ACODT9_00660) coaBC 146303..147529 (+) 1227 WP_046060916.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ACODT9_RS00665 (ACODT9_00665) dut 147498..147953 (+) 456 WP_014146650.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42492.75 Da        Isoelectric Point: 6.3203

>NTDB_id=1111102 ACODT9_RS00640 WP_014146645.1 142488..143621(+) (pilU) [Methylotuvimicrobium alcaliphilum strain 20ZK]
MDEFKRLLELMVQQKASDLFITAGRPPTIKVDGKLIEVSKTILNSDQSRAIVMSVMTQRQIDEFDNTKECQFAIAFPKLG
RFRVSAFTQRDAAGMVLRRIETHIPDAEDLHLPPVLKDLVMNKRGLVLFVGGTGTGKSTSLASLIKYRNQNSSGHIITIE
DPLEFLHPHLGCIVTQREVGMDTESYEVALKNTLRQAPDVILIGEVRTRETMQNAITFAETGHLCITTLHANNANQALDR
ILHFFPDEMHNQLFMDLSLNLRGIVAQQLIARADGKGRYPAVEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQ
ALYDLYTAGKISYEDALNSADSRNEVRLMIKLGAEDLGTLVNENMHLKEDEDEHSRF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1111102 ACODT9_RS00640 WP_014146645.1 142488..143621(+) (pilU) [Methylotuvimicrobium alcaliphilum strain 20ZK]
ATGGATGAATTTAAGCGATTACTTGAGTTAATGGTCCAGCAAAAGGCCTCCGATTTATTTATCACAGCGGGACGCCCGCC
CACGATAAAAGTCGACGGTAAGTTGATCGAAGTGTCCAAAACAATACTAAATAGCGATCAATCACGAGCGATTGTCATGA
GCGTGATGACGCAACGGCAAATAGACGAATTCGATAATACCAAAGAATGCCAGTTTGCGATTGCCTTTCCTAAGCTCGGC
CGATTCCGGGTGAGTGCGTTTACCCAACGAGATGCGGCGGGAATGGTGTTGAGACGTATTGAGACCCATATTCCTGATGC
CGAAGATCTGCATTTGCCGCCGGTTTTAAAAGATTTGGTCATGAACAAGCGCGGCCTGGTGTTGTTTGTCGGCGGGACGG
GAACCGGTAAGTCGACTTCACTGGCGTCGTTGATCAAATACCGAAACCAAAACAGCAGCGGTCATATCATTACCATTGAA
GATCCGCTCGAGTTTTTGCATCCGCACTTGGGGTGTATCGTTACGCAGCGTGAAGTCGGCATGGATACCGAGTCCTATGA
AGTCGCGCTTAAGAATACCTTGCGCCAAGCGCCCGATGTCATCCTGATCGGAGAGGTTAGAACCCGTGAAACGATGCAAA
ATGCGATTACGTTCGCCGAAACCGGACATTTGTGTATAACGACCTTGCATGCGAACAATGCCAATCAGGCATTGGATCGG
ATTTTGCATTTTTTCCCGGACGAAATGCATAATCAATTATTTATGGACTTGTCGTTGAACTTGCGAGGCATTGTCGCGCA
GCAGTTGATTGCCCGTGCCGATGGCAAAGGGCGCTATCCCGCGGTTGAAATTTTGTTGAACACACCCTTGGTTTCCGACT
TGATTCGTAAAGGCGAGGTTCACAAACTTAAGGAATTAATGAAGAACTCGCGGGAACATGGGATGCAGACCTTCGATCAA
GCGCTTTACGATCTTTATACCGCCGGAAAAATTAGCTATGAGGATGCCTTGAACTCTGCTGATTCAAGAAACGAAGTACG
CTTGATGATTAAGCTGGGTGCTGAAGATTTAGGGACTTTAGTCAATGAAAATATGCATTTAAAAGAAGACGAAGACGAAC
ATTCTCGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4SUK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.517

100

0.655
  pilU Acinetobacter baylyi ADP1

63.277

93.899

0.594
  pilU Vibrio cholerae strain A1552

58.166

92.573

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

90.981

0.39
  pilT Legionella pneumophila strain Lp02

42.012

89.655

0.377
  pilT Legionella pneumophila strain ERS1305867

42.012

89.655

0.377

Multiple sequence alignment