Detailed information NTG1110737

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACN8C3_RS19525
• Genome accession: NZ_CP184671
• Coordinates: 3984345..3985325 (+)
• Length: 326 a.a.
• NCBI ID: WP_001326494.1
• Uniprot ID: D3GUL4
• Organism: Escherichia coli HB101
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3979345..3990325

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN8C3_RS19495 (ACN8C3_04085)	yggM	3979860..3980867 (+)	1008	WP_000745210.1	DUF1202 family protein	-
ACN8C3_RS19500 (ACN8C3_04087)	hemW	3981022..3982158 (-)	1137	WP_000239943.1	radical SAM family heme chaperone HemW	-
ACN8C3_RS19505 (ACN8C3_04088)	rdgB	3982151..3982744 (-)	594	WP_001698550.1	XTP/dITP diphosphatase	-
ACN8C3_RS19510 (ACN8C3_04089)	yggU	3982752..3983042 (-)	291	WP_000994920.1	DUF167 family protein YggU	-
ACN8C3_RS19515 (ACN8C3_04090)	yggT	3983039..3983605 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ACN8C3_RS19520 (ACN8C3_04091)	yggS	3983623..3984327 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
ACN8C3_RS19525 (ACN8C3_04092)	pilT	3984345..3985325 (+)	981	WP_001326494.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACN8C3_RS19530 (ACN8C3_04093)	ruvX	3985509..3985925 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
ACN8C3_RS19535 (ACN8C3_04094)	yqgE	3985925..3986488 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
ACN8C3_RS19540 (ACN8C3_04095)	gshB	3986597..3987547 (-)	951	WP_000593273.1	glutathione synthase	-
ACN8C3_RS19545 (ACN8C3_04096)	rsmE	3987560..3988291 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ACN8C3_RS19550 (ACN8C3_04097)	endA	3988371..3989078 (-)	708	WP_000286500.1	deoxyribonuclease I	-
ACN8C3_RS19555 (ACN8C3_04098)	yggI	3989173..3989670 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=1110737 ACN8C3_RS19525 WP_001326494.1 3984345..3985325(+) (pilT) [Escherichia coli HB101]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1110737 ACN8C3_RS19525 WP_001326494.1 3984345..3985325(+) (pilT) [Escherichia coli HB101]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: D3GUL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362