Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMCM8_RS12345 Genome accession   NZ_AP038333
Coordinates   2824697..2825740 (-) Length   347 a.a.
NCBI ID   WP_412722919.1    Uniprot ID   -
Organism   Alteromonas sp. D210916BOD_24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2819697..2830740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMCM8_RS12320 - 2820562..2821578 (-) 1017 WP_412722914.1 GGDEF domain-containing protein -
  ACMCM8_RS12325 (D210916BOD24_24020) - 2822168..2822734 (+) 567 WP_412722915.1 YqgE/AlgH family protein -
  ACMCM8_RS12330 (D210916BOD24_24030) ruvX 2822727..2823158 (+) 432 WP_412722916.1 Holliday junction resolvase RuvX -
  ACMCM8_RS12335 (D210916BOD24_24040) - 2823209..2823535 (-) 327 WP_412722917.1 DUF2007 domain-containing protein -
  ACMCM8_RS12340 (D210916BOD24_24050) pilU 2823576..2824688 (-) 1113 WP_412722918.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMCM8_RS12345 (D210916BOD24_24060) pilT 2824697..2825740 (-) 1044 WP_412722919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMCM8_RS12350 (D210916BOD24_24070) - 2825768..2826469 (+) 702 WP_412722920.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMCM8_RS12355 (D210916BOD24_24080) proC 2826525..2827346 (+) 822 WP_412722921.1 pyrroline-5-carboxylate reductase -
  ACMCM8_RS12360 (D210916BOD24_24090) - 2827363..2827899 (+) 537 WP_412722922.1 YggT family protein -
  ACMCM8_RS12365 (D210916BOD24_24100) - 2827945..2828385 (+) 441 WP_412722923.1 DUF4426 domain-containing protein -
  ACMCM8_RS12370 (D210916BOD24_24110) - 2828437..2830458 (+) 2022 WP_412722924.1 FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38451.21 Da        Isoelectric Point: 6.5685

>NTDB_id=111001 ACMCM8_RS12345 WP_412722919.1 2824697..2825740(-) (pilT) [Alteromonas sp. D210916BOD_24]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHALVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLEELGAPDIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHENKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVISRQDAAEKSIDKKSMGSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=111001 ACMCM8_RS12345 WP_412722919.1 2824697..2825740(-) (pilT) [Alteromonas sp. D210916BOD_24]
GTGGATATTACCGAACTTTTGGCGTTCAGTGTTAAGAATAACGCGTCAGATTTACACTTGTCAGCGGGCCTACCGCCGAT
TATTCGCGTTGATGGTGAAATGCGTAAGCTGAATGTTCCGGCGCTCGATCACAAACAGGTGCATGCGCTTGTTTATGAAA
TTATGAATGACATGCAGCGCAAGGAATACGAAGAAAATCTGGAAACTGATTTCTCTTTTGAAGTGAATGGATTGTCGCGC
TTCCGTGTCAACGCGTTTGTACAAAACCGCGGCGCCGCTGCGGTGCTGCGTACTATCCCCAGTAAAGTGCTGACACTAGA
GGAATTGGGTGCTCCCGATATATTTAAGGAAATTATCAATCAGCCCACCGGTATAGTGCTGGTCACTGGCGCGACAGGCT
CGGGAAAAAGCACGACGCTGGCGGCAATGGTCGATCACATCAATACCCATAAGCGCGAGCATATTCTGACCATTGAAGAT
CCCATTGAATTTGTTCATGAAAACAAGTTAAGCCTTGTCAATCAACGGGAAGTACACAGAGACACTCATTCATTTAACAA
CGCGTTGCGCTCGGCCTTAAGGGAAGATCCCGACGTGATTTTAGTGGGTGAACTACGTGATCTTGAAACCATTCGTTTGG
CGATTTCTGCTGCTGAAACGGGGCATTTAGTTTTCGGCACCTTGCATACTAACTCTGCGCCGAAAACCATCGACCGTATC
ATAGATGTGTTTCCTGCAGAGGAGAAAGCCATGGTGCGCTCGATGTTGTCTGAATCTCTGCGCGCGGTGATATCGCAAAC
CCTACTCAAAAAAGTGGGAGGTGGCCGGGTTGCAGCCCATGAAATCATGGTGGGAATACCGGCTATTCGAAACCTCATAC
GCGAAGACAAAGTGCCGCAAATGTATTCTGTCATTCAGACGGGGCAGGCCCACGGTATGCAAACCATGGATCAATGTCTT
CAGCGGCTGGTGGCGATGGGCGTGATCTCGCGACAAGACGCCGCCGAGAAATCCATCGATAAAAAATCCATGGGCAGTTT
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

78.144

96.254

0.752
  pilT Acinetobacter baumannii D1279779

78.144

96.254

0.752
  pilT Acinetobacter nosocomialis M2

77.844

96.254

0.749
  pilT Pseudomonas stutzeri DSM 10701

76.627

97.406

0.746
  pilT Pseudomonas aeruginosa PAK

76.627

97.406

0.746
  pilT Acinetobacter baylyi ADP1

76.97

95.101

0.732
  pilT Legionella pneumophila strain Lp02

72.141

98.271

0.709
  pilT Legionella pneumophila strain ERS1305867

72.141

98.271

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

65.797

99.424

0.654
  pilT Neisseria gonorrhoeae MS11

65.507

99.424

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.77

98.847

0.522
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.379

100

0.415
  pilU Acinetobacter baylyi ADP1

41.42

97.406

0.403

Multiple sequence alignment