Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMCM8_RS12340 Genome accession   NZ_AP038333
Coordinates   2823576..2824688 (-) Length   370 a.a.
NCBI ID   WP_412722918.1    Uniprot ID   -
Organism   Alteromonas sp. D210916BOD_24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2818576..2829688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMCM8_RS12310 (D210916BOD24_23990) - 2818590..2819318 (+) 729 WP_412722912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACMCM8_RS12315 (D210916BOD24_24000) gshB 2819443..2820396 (+) 954 WP_412722913.1 glutathione synthase -
  ACMCM8_RS12320 - 2820562..2821578 (-) 1017 WP_412722914.1 GGDEF domain-containing protein -
  ACMCM8_RS12325 (D210916BOD24_24020) - 2822168..2822734 (+) 567 WP_412722915.1 YqgE/AlgH family protein -
  ACMCM8_RS12330 (D210916BOD24_24030) ruvX 2822727..2823158 (+) 432 WP_412722916.1 Holliday junction resolvase RuvX -
  ACMCM8_RS12335 (D210916BOD24_24040) - 2823209..2823535 (-) 327 WP_412722917.1 DUF2007 domain-containing protein -
  ACMCM8_RS12340 (D210916BOD24_24050) pilU 2823576..2824688 (-) 1113 WP_412722918.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMCM8_RS12345 (D210916BOD24_24060) pilT 2824697..2825740 (-) 1044 WP_412722919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMCM8_RS12350 (D210916BOD24_24070) - 2825768..2826469 (+) 702 WP_412722920.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMCM8_RS12355 (D210916BOD24_24080) proC 2826525..2827346 (+) 822 WP_412722921.1 pyrroline-5-carboxylate reductase -
  ACMCM8_RS12360 (D210916BOD24_24090) - 2827363..2827899 (+) 537 WP_412722922.1 YggT family protein -
  ACMCM8_RS12365 (D210916BOD24_24100) - 2827945..2828385 (+) 441 WP_412722923.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40821.87 Da        Isoelectric Point: 6.1885

>NTDB_id=111000 ACMCM8_RS12340 WP_412722918.1 2823576..2824688(-) (pilU) [Alteromonas sp. D210916BOD_24]
MLDSLLKKMVEKGASDLFVTADFPVSAKINGKLTPISDGAIDETKSLALVHEAMTDKQRDAFHTSKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPPVLKDIILAKRGLVLFVGGTGTGKSTSLAALIGHRNEHSYGHILTIEDP
IEFVHEHKNCVVTQREVGIDTQSFDEALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKEGKIDMEQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=111000 ACMCM8_RS12340 WP_412722918.1 2823576..2824688(-) (pilU) [Alteromonas sp. D210916BOD_24]
ATGCTAGATTCATTACTTAAAAAGATGGTCGAAAAGGGCGCATCTGACTTATTTGTGACGGCTGACTTTCCGGTTAGCGC
TAAAATTAATGGCAAACTTACCCCTATTTCCGATGGTGCTATCGATGAAACCAAATCATTAGCCTTAGTCCATGAGGCCA
TGACAGACAAGCAACGGGACGCATTTCACACGTCAAAGGAATGTAATTTTGCCATTGTAAGAGAAGGTATTGGGCGTTTT
CGTTGCTCTGCATTTTGGCAGCGAGATCAGGCGGGCATGGTGATCCGTCGCATCGTCACCCAAATCCCTAAAGCTGATGA
TTTGGGGCTCCCGCCGGTATTAAAAGACATTATTTTGGCAAAGCGGGGCTTAGTACTTTTTGTCGGTGGAACGGGCACCG
GAAAGTCCACCTCATTGGCTGCGCTCATCGGTCATCGAAATGAGCATTCCTATGGGCATATCCTGACTATTGAAGATCCT
ATCGAGTTTGTACACGAACACAAAAATTGTGTCGTCACCCAACGCGAAGTAGGTATTGATACTCAGTCTTTTGACGAAGC
GCTAAAAAGCTCGCTGCGCCAGGCGCCTGACGTGATCCTAATTGGTGAAATACGTTCAATGGAAACTATGGAATATGCCA
TGTCGTTTGCCGATACGGGGCATTTATGTGTAGCTACGCTACATGCCAATAATGCTAATCAGGCCATTGAGCGCATTATG
CATTTAGCGCCAAAGGATCAGCATGACAAGCTGCGGTTTGACTTAAGTCTGAACATTCGCGCTATTGTCGCGCAGCAATT
AGTACCCACACCAGATGGAAAAGGGCGAGTGGCGGCAATAGAAATTTTACTTAATTCGCCGTTGGTGCGCGACCTTATTC
AACGCAATGAAATAGGCGCTTTGAAAGAAGCAATGAAGAAAGGCAAAGAGCAGGGAATGCAAAGCTTTGATATGGCGCTG
TATGATTTGTACAAGGAAGGCAAAATTGATATGGAACAAGCGCTGCATCACGCTGATTCACCGAATGATTTGCGTTTAAT
GATCAAGTTAGATACCAGTGACGGATCAAGCTTAGGCACATTGTCTAATGTGTCTATCGACATGGATGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

56.712

98.649

0.559
  pilU Vibrio cholerae strain A1552

55.978

99.459

0.557
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.176

91.892

0.378
  pilT Acinetobacter baumannii strain A118

40.233

92.703

0.373
  pilT Acinetobacter nosocomialis M2

40.233

92.703

0.373
  pilT Acinetobacter baumannii D1279779

40.233

92.703

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.233

92.703

0.373
  pilT Pseudomonas aeruginosa PAK

40.233

92.703

0.373
  pilT Acinetobacter baylyi ADP1

39.942

92.703

0.37
  pilT Legionella pneumophila strain ERS1305867

40.909

89.189

0.365
  pilT Legionella pneumophila strain Lp02

40.909

89.189

0.365

Multiple sequence alignment