Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGHP2_RS03145 Genome accession   NZ_AP035784
Coordinates   661183..662226 (-) Length   347 a.a.
NCBI ID   WP_368353945.1    Uniprot ID   -
Organism   Acidovorax sacchari strain HC-21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 656183..667226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHP2_RS03115 (AVXHC21_06270) - 656375..656782 (+) 408 WP_368353942.1 YraN family protein -
  ACGHP2_RS03120 (AVXHC21_06280) - 656854..657456 (+) 603 WP_053842687.1 SIS domain-containing protein -
  ACGHP2_RS03125 (AVXHC21_06290) - 657453..658109 (+) 657 WP_415267395.1 BON domain-containing protein -
  ACGHP2_RS03130 (AVXHC21_06300) - 658273..659211 (-) 939 WP_368353944.1 NAD(P)-dependent oxidoreductase -
  ACGHP2_RS03135 (AVXHC21_06310) pilU 659295..660431 (-) 1137 WP_013593087.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHP2_RS03140 (AVXHC21_06320) - 660459..661097 (-) 639 WP_013593088.1 cyclic nucleotide-binding domain-containing protein -
  ACGHP2_RS03145 (AVXHC21_06330) pilT 661183..662226 (-) 1044 WP_368353945.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHP2_RS03150 (AVXHC21_06340) - 662268..662993 (+) 726 WP_368353946.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHP2_RS03155 (AVXHC21_06350) ltaE 663005..664069 (-) 1065 WP_107132100.1 low-specificity L-threonine aldolase -
  ACGHP2_RS03160 (AVXHC21_06360) - 664291..665430 (+) 1140 WP_415267396.1 aminotransferase class V-fold PLP-dependent enzyme -
  ACGHP2_RS03165 (AVXHC21_06370) - 665546..666970 (+) 1425 WP_368353948.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38104.92 Da        Isoelectric Point: 6.8354

>NTDB_id=110817 ACGHP2_RS03145 WP_368353945.1 661183..662226(-) (pilT) [Acidovorax sacchari strain HC-21]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=110817 ACGHP2_RS03145 WP_368353945.1 661183..662226(-) (pilT) [Acidovorax sacchari strain HC-21]
GTGGACATCACCCAACTGCTCGCCTTCAGCGTGAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGCCTGCCTCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACGTCGATGCGCTCGACCACAAGACGGTGCATGCCATGGTGTACGACA
TCATGAGCGACGCCCAGCGCAAGGTGTACGAGGAGTTCCTGGAAGTGGACTTCTCGTTCGAGATCGAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGTACCATCCCGAGCAAGATCCTCACGCTCGA
GCAGCTCAACGCCCCGAAAATCTTCGCCGACCTGGCCCTCAAGCCGCGCGGCCTCGTGCTCGTCACCGGCCCCACGGGCT
CGGGCAAGTCCACTACGCTCGCGGCCATGGTGAACCACCTCAACGAGACCGAGTACGGGCATATCCTGACCGTGGAAGAC
CCGATCGAGTTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCGC
CGCGCTGAAATCCGCGCTGCGCGAAGACCCGGACGCCATCCTCGTGGGTGAAATGCGCGACCTCGAGACCATCCGGCTGG
CCATGACCGCCGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAAGAGAAGGAGATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCCCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCAGTGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAGCAGCGTCGGCATGCAGACGCTGGACCAGAAC
CTCACCGACCTCGTGCGGCGCAACATCATCAGCCCCGCGGAAGCCCGCGGCAAGGCCAAGATTCCGGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment