Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHP2_RS03135 Genome accession   NZ_AP035784
Coordinates   659295..660431 (-) Length   378 a.a.
NCBI ID   WP_013593087.1    Uniprot ID   F0Q6A0
Organism   Acidovorax sacchari strain HC-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 654295..665431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHP2_RS03110 (AVXHC21_06260) rsmI 655416..656375 (-) 960 WP_368353941.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACGHP2_RS03115 (AVXHC21_06270) - 656375..656782 (+) 408 WP_368353942.1 YraN family protein -
  ACGHP2_RS03120 (AVXHC21_06280) - 656854..657456 (+) 603 WP_053842687.1 SIS domain-containing protein -
  ACGHP2_RS03125 (AVXHC21_06290) - 657453..658109 (+) 657 WP_415267395.1 BON domain-containing protein -
  ACGHP2_RS03130 (AVXHC21_06300) - 658273..659211 (-) 939 WP_368353944.1 NAD(P)-dependent oxidoreductase -
  ACGHP2_RS03135 (AVXHC21_06310) pilU 659295..660431 (-) 1137 WP_013593087.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHP2_RS03140 (AVXHC21_06320) - 660459..661097 (-) 639 WP_013593088.1 cyclic nucleotide-binding domain-containing protein -
  ACGHP2_RS03145 (AVXHC21_06330) pilT 661183..662226 (-) 1044 WP_368353945.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHP2_RS03150 (AVXHC21_06340) - 662268..662993 (+) 726 WP_368353946.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHP2_RS03155 (AVXHC21_06350) ltaE 663005..664069 (-) 1065 WP_107132100.1 low-specificity L-threonine aldolase -
  ACGHP2_RS03160 (AVXHC21_06360) - 664291..665430 (+) 1140 WP_415267396.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41718.14 Da        Isoelectric Point: 6.9079

>NTDB_id=110816 ACGHP2_RS03135 WP_013593087.1 659295..660431(-) (pilU) [Acidovorax sacchari strain HC-21]
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVIGYEDALRNADSLNDLRLQIKLGSQRARSTDLSSGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=110816 ACGHP2_RS03135 WP_013593087.1 659295..660431(-) (pilU) [Acidovorax sacchari strain HC-21]
ATGGAACGCGATCAGGCCAGCAAATTCATCAACGACCTGCTGAAGCTCATGGTGGGGCGCGGCGGCAGCGACCTTTTCAT
CACGGCGGAATTCCCCCCCGCCATCAAGGTGGACGGAAAGGTCACGAAGGTCTCCCCCCAGCCGCTCACGCCGGCGCACA
CCCTCACCCTCGCGCGCGCCATCATGAGCGACAAGCAGGTGGCGGACTTCGAGCGTACCAAGGAGTGCAACTTCGCCATC
TCCCCAGCGGGCATCGGCCGCTTCCGCGTGAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGCTGCGAACCATCCC
GCTCACGCTGCCCACCATCGACGGGCTGGGCGTGCCGCAGGTGCTCAAGGAAATCACCATGTCCAAGCGCGGGCTGTGCA
TCCTCGTGGGCGCGACCGGCTCGGGCAAGTCCACCACCCTCGCGGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCGATCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTGACCCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCCGCCCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATGGGCGAAATCCGCG
ACCGCGAGACCATGGACCATGCCATCGCCTTCTCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAGGAACGGCGCGCGCAGTTGCTCATGGACCTGTCGCTCAACCT
GCGTGCCATGGTGTCCCAGCGCCTCATCCCCAAGCAGGACGGCAAGGGCCGCGCCGCGGCGGTGGAGGTCATGCTCAACA
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGTCGCTCTTCGACCTCTTCGAGGCCAACGTCATCGGCTACGAGGACGCCCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGTGCCCGCAGCACCGACCTGTCGTCCGGCACCGAGC
ACTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0Q6A0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment