Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN19N_RS03975 Genome accession   NZ_CP184160
Coordinates   822364..823401 (-) Length   345 a.a.
NCBI ID   WP_269229785.1    Uniprot ID   -
Organism   Acinetobacter sp. LF10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817364..828401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN19N_RS03945 (ACN19N_03945) - 818014..818469 (+) 456 WP_269229781.1 bacteriohemerythrin -
  ACN19N_RS03950 (ACN19N_03950) - 818718..819800 (+) 1083 WP_269239198.1 hypothetical protein -
  ACN19N_RS03955 (ACN19N_03955) - 819800..820120 (+) 321 WP_269239199.1 RnfH family protein -
  ACN19N_RS03960 (ACN19N_03960) - 820179..820577 (-) 399 WP_269229784.1 outer membrane protein assembly factor BamE -
  ACN19N_RS03965 (ACN19N_03965) fur 820689..821126 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  ACN19N_RS03970 (ACN19N_03970) pilU 821221..822339 (-) 1119 WP_004663427.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN19N_RS03975 (ACN19N_03975) pilT 822364..823401 (-) 1038 WP_269229785.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN19N_RS03980 (ACN19N_03980) - 823526..824218 (+) 693 WP_420002000.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN19N_RS03985 (ACN19N_03985) - 824402..825124 (+) 723 WP_420002001.1 hypothetical protein -
  ACN19N_RS03990 (ACN19N_03990) - 825149..825721 (-) 573 WP_420002002.1 hypothetical protein -
  ACN19N_RS03995 (ACN19N_03995) - 825960..826169 (-) 210 WP_420002003.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38270.87 Da        Isoelectric Point: 6.5054

>NTDB_id=1108012 ACN19N_RS03975 WP_269229785.1 822364..823401(-) (pilT) [Acinetobacter sp. LF10]
MDITELLAFAVKNGASDLHLSSGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1108012 ACN19N_RS03975 WP_269229785.1 822364..823401(-) (pilT) [Acinetobacter sp. LF10]
ATGGATATTACAGAATTATTAGCATTTGCAGTTAAAAACGGTGCTTCAGACTTACATCTTTCATCAGGTATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAATTTACCAGCACTAGAACATAAAGATGTACACCGCTTAGTGTATGACA
TCATGAATGATAAACAACGTCGTGATTATGAAGAAGATCTCGAAACTGATTTTTCATTTGAAGTGCCTAATATTGCACGT
TTCCGTGTGAACGCATTCAACCAAAACCGTGGCGCTGGCGCAGTATTCCGTACCATTCCATCTAAAGTTTTAACCATGGA
AGACCTTGGTTTAGGTTCAATTTTTAAAGAAATTTGTGATTATCCACGTGGTATCGTTTTGGTTACAGGACCTACGGGTT
CTGGTAAATCAACGACGCTCGCTGCGATGATTGATTATATTAATGAAAACCGTTATGACCATATTTTAACAGTTGAAGAC
CCGATTGAATTTGTACACCAATCTAAAAAATGTTTGATTAACCAACGTGAAGTGCATCGTGATACTCATGGTTTTAATGC
CGCACTTCGTTCTGCGCTTCGTGAAGACCCAGACATCATACTTGTCGGTGAGATGCGTGACTTAGAAACCATTCGACTTG
CGTTAACTGCGGCTGAAACAGGTCACTTGGTTTTTGGTACCCTACATACCACCTCTGCAGCTAAAACCATTGACCGTGTC
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGTGCCATGCTTTCTGAATCACTACAAGCCGTTATTTCTCAAAC
ATTATTGAAGAAAAATGGTGGTGGTCGTGTTGCAGCGCATGAAATCATGATTGGAATTCCAGCCATTCGTAACCTTGTAC
GTGAAAATAAAGTAGCTCAGATGTATTCTGCCATCCAAACGGGTGCAAACTACGGTATGACTACACTTGACCAAAGTCTT
AAGACCTTAGTTTCAAAAGGCGTTATTAGCCCTCAAGTTGCACGTACTGCAGCAAAACAACCTGAGTCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Pseudomonas aeruginosa PAK

82.267

99.71

0.82
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.696

100

0.687
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.785

95.942

0.391
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment