Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACN19N_RS03970 Genome accession   NZ_CP184160
Coordinates   821221..822339 (-) Length   372 a.a.
NCBI ID   WP_004663427.1    Uniprot ID   N9LTH9
Organism   Acinetobacter sp. LF10     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816221..827339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN19N_RS03940 (ACN19N_03940) - 816889..817737 (+) 849 WP_269229780.1 class II glutamine amidotransferase -
  ACN19N_RS03945 (ACN19N_03945) - 818014..818469 (+) 456 WP_269229781.1 bacteriohemerythrin -
  ACN19N_RS03950 (ACN19N_03950) - 818718..819800 (+) 1083 WP_269239198.1 hypothetical protein -
  ACN19N_RS03955 (ACN19N_03955) - 819800..820120 (+) 321 WP_269239199.1 RnfH family protein -
  ACN19N_RS03960 (ACN19N_03960) - 820179..820577 (-) 399 WP_269229784.1 outer membrane protein assembly factor BamE -
  ACN19N_RS03965 (ACN19N_03965) fur 820689..821126 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  ACN19N_RS03970 (ACN19N_03970) pilU 821221..822339 (-) 1119 WP_004663427.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN19N_RS03975 (ACN19N_03975) pilT 822364..823401 (-) 1038 WP_269229785.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN19N_RS03980 (ACN19N_03980) - 823526..824218 (+) 693 WP_420002000.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN19N_RS03985 (ACN19N_03985) - 824402..825124 (+) 723 WP_420002001.1 hypothetical protein -
  ACN19N_RS03990 (ACN19N_03990) - 825149..825721 (-) 573 WP_420002002.1 hypothetical protein -
  ACN19N_RS03995 (ACN19N_03995) - 825960..826169 (-) 210 WP_420002003.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41655.82 Da        Isoelectric Point: 6.4346

>NTDB_id=1108011 ACN19N_RS03970 WP_004663427.1 821221..822339(-) (pilU) [Acinetobacter sp. LF10]
MDFNDLLNLMIQQSASDLFVTADVEPSMKINGQIVPVSKSKLSGEVVGQLLHSIMSDKQRKEFAETRECNFAIVNREKTA
RFRVSAFQQRDMPGMVLRRIETVIPTMDELKLPSVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHPQLHMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGTDQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1108011 ACN19N_RS03970 WP_004663427.1 821221..822339(-) (pilU) [Acinetobacter sp. LF10]
ATGGACTTTAATGATTTACTCAATTTAATGATTCAACAAAGTGCATCTGATTTATTTGTCACTGCAGATGTTGAACCCTC
AATGAAGATCAACGGTCAAATTGTGCCTGTTTCAAAATCTAAACTCTCAGGTGAAGTCGTTGGTCAGTTACTTCATTCAA
TTATGTCAGATAAGCAGCGTAAAGAATTTGCTGAAACACGTGAATGCAACTTTGCGATTGTCAATCGTGAAAAAACGGCA
CGTTTCCGTGTGAGTGCTTTTCAACAGCGTGATATGCCTGGGATGGTTTTACGTCGTATTGAGACTGTCATTCCAACCAT
GGATGAACTCAAGCTCCCTAGCGTGCTGAAAGAACTTGCCATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACTG
GTACAGGTAAATCAACCTCATTAGCTTCGATGATTGGTTACCGTAACCAAAACTCAAAGGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTACATCAACATGCGGGTTGTATTGTCACTCAACGTGAAGTTGGGATTGATACAGATTCATTTGA
AGTCGCATTAAAAAATACGCTTCGCCAAGCACCTGATGTGATCTTAATTGGTGAGATTCGTTCTCGTGAAACCATGGACT
ATGCAATTGCTTTTGCTGAAACTGGTCACTTAGTGCTCGCAACACTACACGCAAACAACGCGAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAGCAGATCGTCATCCTCAATTGCATATGGATTTATCACTCAATCTCAAGGCGATGGTTGCTCA
ACAGTTGATCCCAACGCCAGATGGTAATTCACGTCGCGCAGCAATTGAGATTCTGATTAATACACCACTTTTGGCAGACT
ACATACGCAAAGGTGAAGTACACGAAATCAAAGATCTCATGAAGCGTTCTCGAGAATTGGGGATGCAGACTTTTGACCAA
GCATTATTTGATTTATACAAAGCAGGTGAAATTACGTATAAAGATGCACTGAAGCATGCTGACTCGCCAAATGACTTACG
TCTCACAATTAAACTTTCAGAAGAAGGTACAGATCAGTTATTGAGTGCGAGCCGAAATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LTH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.661

100

0.769
  pilU Pseudomonas stutzeri DSM 10701

71.429

99.731

0.712
  pilU Vibrio cholerae strain A1552

55.676

99.462

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.209

97.849

0.403
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Neisseria gonorrhoeae MS11

40

91.398

0.366
  pilT Neisseria meningitidis 8013

39.766

91.935

0.366
  pilT Acinetobacter baumannii D1279779

39.359

92.204

0.363
  pilT Acinetobacter baumannii strain A118

39.359

92.204

0.363
  pilT Acinetobacter nosocomialis M2

39.359

92.204

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Legionella pneumophila strain Lp02

41.284

87.903

0.363
  pilT Legionella pneumophila strain ERS1305867

41.284

87.903

0.363

Multiple sequence alignment