Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN429_RS20380 Genome accession   NZ_CP183397
Coordinates   4356145..4357179 (-) Length   344 a.a.
NCBI ID   WP_007161666.1    Uniprot ID   -
Organism   Pseudomonas oryzihabitans strain CCSM P719     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4351145..4362179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN429_RS20360 (ACN429_20360) - 4351506..4352213 (+) 708 WP_037007725.1 C40 family peptidase -
  ACN429_RS20365 (ACN429_20365) - 4352346..4353557 (+) 1212 WP_027602931.1 putative DNA modification/repair radical SAM protein -
  ACN429_RS20370 (ACN429_20370) - 4353558..4355006 (+) 1449 WP_044341946.1 UdgX family uracil-DNA binding protein -
  ACN429_RS20375 (ACN429_20375) pilU 4355003..4356145 (-) 1143 WP_007161667.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN429_RS20380 (ACN429_20380) pilT 4356145..4357179 (-) 1035 WP_007161666.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN429_RS20385 (ACN429_20385) - 4357306..4358001 (+) 696 WP_027602929.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN429_RS20390 (ACN429_20390) proC 4358011..4358829 (+) 819 WP_420030568.1 pyrroline-5-carboxylate reductase -
  ACN429_RS20395 (ACN429_20395) - 4358839..4359432 (+) 594 WP_007161663.1 YggT family protein -
  ACN429_RS20400 (ACN429_20400) - 4359550..4361508 (+) 1959 WP_044341948.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37941.73 Da        Isoelectric Point: 6.8363

>NTDB_id=1105548 ACN429_RS20380 WP_007161666.1 4356145..4357179(-) (pilT) [Pseudomonas oryzihabitans strain CCSM P719]
MDITELLAFSARQGASDLHLSAGLPPMIRVDGDVRRINLPAFEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGGLGMQTLDMCL
KALLAKGLISRDAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1105548 ACN429_RS20380 WP_007161666.1 4356145..4357179(-) (pilT) [Pseudomonas oryzihabitans strain CCSM P719]
ATGGATATCACCGAATTGCTGGCCTTTTCCGCGCGGCAAGGCGCCTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGGCCTTCGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTGGAGACCGACTTCTCCTTCGAGGTGCCGGGTGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGTGCCGGCGCGGTGTTCCGAACCATTCCGTCCAAGGTGCTGAGCATGGA
AGAGCTGGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTCCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGCTCGACTACCTCAACAACACCAAGTACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTCCACGAATCCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTAGGCGAACTGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGGCACCTGGTCTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGATCGGGTG
GTGGACGTCTTTCCGGCGGAAGAGAAGTCGATGGTGCGTTCCATGTTGTCCGAATCCCTGCAGGCGGTGGTTTCCCAGAC
CCTGCTCAAGAAGGTCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACGCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGTGGCGGCCTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGCGCTCCTGGCCAAGGGCCTGATCAGCCGCGACGCGGCCCGCGAGAAGGCCAAGACGCCGGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.64

100

0.956
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

82.558

100

0.826
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment