Detailed information NTG1105547

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACN429_RS20375
• Genome accession: NZ_CP183397
• Coordinates: 4355003..4356145 (-)
• Length: 380 a.a.
• NCBI ID: WP_007161667.1
• Uniprot ID: A0A329I0Q7
• Organism: Pseudomonas oryzihabitans strain CCSM P719
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4350003..4361145

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN429_RS20355 (ACN429_20355)	-	4350820..4351224 (-)	405	WP_007161671.1	NINE protein	-
ACN429_RS20360 (ACN429_20360)	-	4351506..4352213 (+)	708	WP_037007725.1	C40 family peptidase	-
ACN429_RS20365 (ACN429_20365)	-	4352346..4353557 (+)	1212	WP_027602931.1	putative DNA modification/repair radical SAM protein	-
ACN429_RS20370 (ACN429_20370)	-	4353558..4355006 (+)	1449	WP_044341946.1	UdgX family uracil-DNA binding protein	-
ACN429_RS20375 (ACN429_20375)	pilU	4355003..4356145 (-)	1143	WP_007161667.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACN429_RS20380 (ACN429_20380)	pilT	4356145..4357179 (-)	1035	WP_007161666.1	type IV pilus twitching motility protein PilT	Machinery gene
ACN429_RS20385 (ACN429_20385)	-	4357306..4358001 (+)	696	WP_027602929.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACN429_RS20390 (ACN429_20390)	proC	4358011..4358829 (+)	819	WP_420030568.1	pyrroline-5-carboxylate reductase	-
ACN429_RS20395 (ACN429_20395)	-	4358839..4359432 (+)	594	WP_007161663.1	YggT family protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42466.89 Da
• Isoelectric Point: 7.3063

>NTDB_id=1105547 ACN429_RS20375 WP_007161667.1 4355003..4356145(-) (pilU) [Pseudomonas oryzihabitans strain CCSM P719]
MDFDRFLQLMVDKGASDLFITTGVPPSMKINGRMTPVTKDSLTPEQCRALVLGVMNDEQRQEFQQKRECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIEVNIPTVEQLRLPEIIKKLAMTKRGLVIFVGATGTGKSTSLAAMIGHRNKNSAGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEIRSRDTMEHALAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQTWMDLSLNLKGIVAQQLVPTIDGQGRRAVIEVLLNTPLVADLIRKGEVHELKALMKKSGELGMQTFDQS
LYGLYRDGEITYEAALAHADSANDLRLMIKLGSETDADHLNQITRNLSVDMGDEPPKRFR

Nucleotide

• Length: 1143 bp

>NTDB_id=1105547 ACN429_RS20375 WP_007161667.1 4355003..4356145(-) (pilU) [Pseudomonas oryzihabitans strain CCSM P719]
ATGGATTTCGACAGGTTCCTGCAACTGATGGTGGACAAGGGGGCGTCCGACCTCTTCATCACCACCGGCGTGCCCCCTTC
GATGAAGATCAACGGGCGCATGACGCCGGTCACCAAGGACTCGCTCACGCCCGAGCAGTGCCGGGCCCTGGTGCTGGGGG
TGATGAACGACGAGCAGCGCCAGGAATTCCAGCAGAAGCGCGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGGTCAACATCCCCACCGTCGA
GCAGCTGCGCCTGCCGGAGATCATCAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCCATGATCGGCCACCGCAACAAGAACTCGGCGGGGCACATCATCTCCATCGAGGAC
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTTACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTCATCATGATCGGCGAGATCCGCAGCCGGGACACCATGGAGCACG
CCCTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCCACTTCTTCCCGGCCGATCGGCACAGCCAGACCTGGATGGACCTCTCACTCAACCTCAAGGGCATCGTCGCCCAGCA
ACTGGTGCCCACCATCGATGGCCAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTCAACACCCCGCTGGTCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGCGCTGATGAAGAAGTCCGGCGAGCTGGGCATGCAGACCTTCGACCAGTCG
CTCTACGGGCTCTATCGTGATGGCGAGATCACCTACGAGGCCGCCCTGGCCCACGCCGACTCCGCCAATGACCTGCGCCT
GATGATCAAGCTGGGTTCGGAAACGGATGCCGATCACCTCAATCAGATCACCCGCAACCTCTCGGTGGACATGGGCGACG
AGCCGCCCAAGCGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A329I0Q7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	77.428	100	0.776
pilU	Acinetobacter baylyi ADP1	62.5	98.947	0.618
pilU	Vibrio cholerae strain A1552	56.873	97.632	0.555
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	89.211	0.382