Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN0NO_RS04935 Genome accession   NZ_CP183298
Coordinates   1056976..1058013 (+) Length   345 a.a.
NCBI ID   WP_068849741.1    Uniprot ID   A0AAP5AKB6
Organism   Stenotrophomonas rhizophila strain SJ-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1051976..1063013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN0NO_RS04915 (ACN0NO_04915) - 1053417..1054775 (-) 1359 WP_095363719.1 MFS transporter -
  ACN0NO_RS04920 (ACN0NO_04920) soxR 1054865..1055299 (+) 435 WP_176472643.1 redox-sensitive transcriptional activator SoxR -
  ACN0NO_RS04925 (ACN0NO_04925) proC 1055306..1056124 (-) 819 WP_095363720.1 pyrroline-5-carboxylate reductase -
  ACN0NO_RS04930 (ACN0NO_04930) - 1056189..1056860 (-) 672 WP_176472644.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN0NO_RS04935 (ACN0NO_04935) pilT 1056976..1058013 (+) 1038 WP_068849741.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN0NO_RS04940 (ACN0NO_04940) pilU 1058140..1059282 (+) 1143 WP_068849744.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN0NO_RS04945 (ACN0NO_04945) - 1059398..1059982 (+) 585 WP_095363721.1 hypothetical protein -
  ACN0NO_RS04950 (ACN0NO_04950) - 1060113..1061096 (+) 984 WP_095363722.1 aldo/keto reductase -
  ACN0NO_RS04955 (ACN0NO_04955) - 1061161..1061376 (+) 216 WP_068854565.1 DUF4287 domain-containing protein -
  ACN0NO_RS04960 (ACN0NO_04960) - 1061471..1062178 (+) 708 WP_068849751.1 YitT family protein -
  ACN0NO_RS04965 (ACN0NO_04965) - 1062183..1062710 (-) 528 WP_095363723.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38640.39 Da        Isoelectric Point: 6.5074

>NTDB_id=1105144 ACN0NO_RS04935 WP_068849741.1 1056976..1058013(+) (pilT) [Stenotrophomonas rhizophila strain SJ-3]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLNTPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQMGMMTLDQHL
QDLVKRSLITRNQAKEYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1105144 ACN0NO_RS04935 WP_068849741.1 1056976..1058013(+) (pilT) [Stenotrophomonas rhizophila strain SJ-3]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTTAAGAACAAAGCGTCGGACCTTCATCTGTCGGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGATGTCCGTCGCATCAACATTCCGGCCTTGGACCACAAGCAGGTCCACGCGCTCGTTTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGAACACGCCGCCGATCTTCCGCGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
AGCACTGCGTTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAACTCCGCGACCTGGAAACCATCCGGCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCCATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
GCTGCTGAAGAAAGTGGGCGGCGGGCGCACCGCCGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGATGGGCATGATGACGCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCCAAGGAATACGCCAAGGACAAGCGCATCTTCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment