Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACN0NO_RS04940 Genome accession   NZ_CP183298
Coordinates   1058140..1059282 (+) Length   380 a.a.
NCBI ID   WP_068849744.1    Uniprot ID   -
Organism   Stenotrophomonas rhizophila strain SJ-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1053140..1064282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN0NO_RS04915 (ACN0NO_04915) - 1053417..1054775 (-) 1359 WP_095363719.1 MFS transporter -
  ACN0NO_RS04920 (ACN0NO_04920) soxR 1054865..1055299 (+) 435 WP_176472643.1 redox-sensitive transcriptional activator SoxR -
  ACN0NO_RS04925 (ACN0NO_04925) proC 1055306..1056124 (-) 819 WP_095363720.1 pyrroline-5-carboxylate reductase -
  ACN0NO_RS04930 (ACN0NO_04930) - 1056189..1056860 (-) 672 WP_176472644.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN0NO_RS04935 (ACN0NO_04935) pilT 1056976..1058013 (+) 1038 WP_068849741.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN0NO_RS04940 (ACN0NO_04940) pilU 1058140..1059282 (+) 1143 WP_068849744.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN0NO_RS04945 (ACN0NO_04945) - 1059398..1059982 (+) 585 WP_095363721.1 hypothetical protein -
  ACN0NO_RS04950 (ACN0NO_04950) - 1060113..1061096 (+) 984 WP_095363722.1 aldo/keto reductase -
  ACN0NO_RS04955 (ACN0NO_04955) - 1061161..1061376 (+) 216 WP_068854565.1 DUF4287 domain-containing protein -
  ACN0NO_RS04960 (ACN0NO_04960) - 1061471..1062178 (+) 708 WP_068849751.1 YitT family protein -
  ACN0NO_RS04965 (ACN0NO_04965) - 1062183..1062710 (-) 528 WP_095363723.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42315.70 Da        Isoelectric Point: 6.7684

>NTDB_id=1105145 ACN0NO_RS04940 WP_068849744.1 1058140..1059282(+) (pilU) [Stenotrophomonas rhizophila strain SJ-3]
MNTTSTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNLNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPSPDGKSRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQLG
MRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1105145 ACN0NO_RS04940 WP_068849744.1 1058140..1059282(+) (pilU) [Stenotrophomonas rhizophila strain SJ-3]
ATGAACACCACCAGCACCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTTTTCAT
CACCGCGGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCCATCACCCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAAGAGTTCGAAAAAACCCACGAGTGCAACTTCGCCATC
GGCCTGTCCGGCGTGGGCCGGTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTCCTGCGCCGCATCGA
AACCCGCATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTGGGTGCGACCGGTACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCTGAATTCCACCGGG
CACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTGGGCAT
CGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGTA
CCCGCGAAGGCATGGACCATGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGTACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAGCCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGGCGTGGTCGCCCAGCAGCTCGTGCCTTCGCCGGATGGCAAGTCGCGCAAGGTGGCGATGGAAATCATGCTGGGCA
CGCCGCTGGTGCAGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCGGTGCAGCTGGGC
ATGCGCACCTTCGACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGA
TTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGACGCACGCACGCTGTCGCAGGGCCTGGACGGCG
TGGAGATTTCCGAAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.579

0.653
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.179

94.211

0.397
  pilT Acinetobacter baumannii strain A118

40.407

90.526

0.366
  pilT Acinetobacter nosocomialis M2

40.407

90.526

0.366
  pilT Acinetobacter baumannii D1279779

40.407

90.526

0.366
  pilT Legionella pneumophila strain ERS1305867

41.194

88.158

0.363
  pilT Legionella pneumophila strain Lp02

41.194

88.158

0.363

Multiple sequence alignment