Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN08L_RS02865 Genome accession   NZ_CP183285
Coordinates   467815..468852 (+) Length   345 a.a.
NCBI ID   WP_008987964.1    Uniprot ID   A0A0U1P8W8
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sr3.21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 462815..473852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08L_RS02830 (ACN08L_02825) hemW 462875..464038 (-) 1164 WP_419237165.1 radical SAM family heme chaperone HemW -
  ACN08L_RS02835 (ACN08L_02830) - 464039..464632 (-) 594 WP_045064316.1 XTP/dITP diphosphatase -
  ACN08L_RS02840 (ACN08L_02835) - 464752..465177 (-) 426 WP_008987959.1 DUF4426 domain-containing protein -
  ACN08L_RS02845 (ACN08L_02840) yggU 465323..465616 (-) 294 WP_419237166.1 DUF167 family protein YggU -
  ACN08L_RS02850 (ACN08L_02845) - 465613..466167 (-) 555 WP_008987961.1 YggT family protein -
  ACN08L_RS02855 (ACN08L_02850) proC 466177..466995 (-) 819 WP_107218745.1 pyrroline-5-carboxylate reductase -
  ACN08L_RS02860 (ACN08L_02855) - 467082..467792 (-) 711 WP_008987963.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN08L_RS02865 (ACN08L_02860) pilT 467815..468852 (+) 1038 WP_008987964.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN08L_RS02870 (ACN08L_02865) pilU 468887..469990 (+) 1104 WP_008987965.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN08L_RS02875 (ACN08L_02870) ruvX 470331..470759 (-) 429 WP_023933568.1 Holliday junction resolvase RuvX -
  ACN08L_RS02880 (ACN08L_02875) - 470756..471319 (-) 564 WP_008987967.1 YqgE/AlgH family protein -
  ACN08L_RS02885 (ACN08L_02880) gshB 471443..472393 (-) 951 WP_006647266.1 glutathione synthase -
  ACN08L_RS02890 (ACN08L_02885) rsmE 472412..473143 (-) 732 WP_045069248.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38372.91 Da        Isoelectric Point: 6.0865

>NTDB_id=1104993 ACN08L_RS02865 WP_008987964.1 467815..468852(+) (pilT) [Photobacterium leiognathi subsp. mandapamensis strain Sr3.21]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSMPALDHSEVHRLVFDIMNDAQRREYEENLEVDFSFEIPDIGR
FRVNAFHQSRGCSAVLRTIPIHIPTLESLNVPDVFYDIAQLKRGLVLVTGATGSGKSTTIAALVDYINSNYNRHILTIED
PIEFVHQSKRCLINQREVHRDTLGFQNALRSALREDPDVILVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGADKSMVRSMLSESLRAVISQSLLKRVEGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSSQRGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1104993 ACN08L_RS02865 WP_008987964.1 467815..468852(+) (pilT) [Photobacterium leiognathi subsp. mandapamensis strain Sr3.21]
ATGGATATCACCGAACTACTGGATTTTAGTGTAAAGCATAACGCATCGGATCTACATCTTTCTGCAGGTGTTCCTCCTAT
GATCCGTGTCGATGGCGATGTGAGAAAATTAAGCATGCCAGCGTTAGATCATAGTGAAGTGCATCGCTTAGTGTTTGACA
TTATGAACGATGCCCAACGTCGTGAGTATGAAGAAAACTTAGAAGTCGATTTTTCATTTGAAATTCCTGACATTGGTCGC
TTCAGGGTTAATGCTTTCCACCAATCTCGTGGTTGCTCTGCTGTATTACGTACGATCCCAATCCATATTCCGACATTGGA
ATCGTTAAATGTGCCTGACGTGTTCTACGACATTGCTCAGCTTAAGCGTGGCTTAGTGCTGGTAACGGGGGCAACAGGTT
CAGGTAAATCGACCACAATTGCTGCCTTGGTTGATTATATCAATAGCAACTATAACCGTCATATTTTGACTATCGAAGAT
CCGATCGAATTTGTGCACCAAAGCAAGCGTTGTTTGATCAACCAGCGTGAAGTGCACCGTGATACGTTAGGTTTCCAGAA
TGCATTGCGCTCAGCACTGCGTGAAGATCCAGACGTTATTTTGGTTGGTGAATTACGTGATCAAGAAACCATTAGCTTAG
CGCTCACCGCAGCAGAAACTGGTCACTTAGTGTTGGGTACGCTACACACCAGCTCGGCGGCGAAAACAGTCGACCGTATT
ATTGACGTATTCCCAGGTGCAGATAAATCGATGGTGCGCTCAATGTTGTCTGAATCACTGCGTGCGGTAATTTCTCAAAG
CCTGTTAAAGCGTGTCGAAGGTGGTCGTGTTGCATCTCATGAAATCATGATGGCAACCCCTGCGATCCGAAATTTGATCC
GTGAAGATAAGATCGCGCAAATGTACTCGATGATCCAAACCGGCTCAGCAATGGGCATGCAGACGATGGAGCAAAGCGTC
AAGATGCTGGTGGCGCAAGGTTTAGTGGAAGCTGAAGAAGGGCGTCGTATTGTTGATAGTAGTCAGCGAGGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U1P8W8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.812

100

0.768
  pilT Vibrio cholerae strain A1552

76.812

100

0.768
  pilT Acinetobacter baumannii strain A118

71.045

97.101

0.69
  pilT Acinetobacter baumannii D1279779

71.045

97.101

0.69
  pilT Acinetobacter baylyi ADP1

69.298

99.13

0.687
  pilT Acinetobacter nosocomialis M2

70.746

97.101

0.687
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.101

0.678
  pilT Pseudomonas aeruginosa PAK

69.254

97.101

0.672
  pilT Legionella pneumophila strain Lp02

69.605

95.362

0.664
  pilT Legionella pneumophila strain ERS1305867

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

65.306

99.42

0.649
  pilT Neisseria gonorrhoeae MS11

65.015

99.42

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.419

99.71

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.566

99.42

0.423
  pilU Vibrio cholerae strain A1552

41.52

99.13

0.412
  pilU Acinetobacter baylyi ADP1

39.752

93.333

0.371

Multiple sequence alignment