Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACN08L_RS02870 Genome accession   NZ_CP183285
Coordinates   468887..469990 (+) Length   367 a.a.
NCBI ID   WP_008987965.1    Uniprot ID   A0A0U1P8H5
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sr3.21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463887..474990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08L_RS02835 (ACN08L_02830) - 464039..464632 (-) 594 WP_045064316.1 XTP/dITP diphosphatase -
  ACN08L_RS02840 (ACN08L_02835) - 464752..465177 (-) 426 WP_008987959.1 DUF4426 domain-containing protein -
  ACN08L_RS02845 (ACN08L_02840) yggU 465323..465616 (-) 294 WP_419237166.1 DUF167 family protein YggU -
  ACN08L_RS02850 (ACN08L_02845) - 465613..466167 (-) 555 WP_008987961.1 YggT family protein -
  ACN08L_RS02855 (ACN08L_02850) proC 466177..466995 (-) 819 WP_107218745.1 pyrroline-5-carboxylate reductase -
  ACN08L_RS02860 (ACN08L_02855) - 467082..467792 (-) 711 WP_008987963.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN08L_RS02865 (ACN08L_02860) pilT 467815..468852 (+) 1038 WP_008987964.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN08L_RS02870 (ACN08L_02865) pilU 468887..469990 (+) 1104 WP_008987965.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN08L_RS02875 (ACN08L_02870) ruvX 470331..470759 (-) 429 WP_023933568.1 Holliday junction resolvase RuvX -
  ACN08L_RS02880 (ACN08L_02875) - 470756..471319 (-) 564 WP_008987967.1 YqgE/AlgH family protein -
  ACN08L_RS02885 (ACN08L_02880) gshB 471443..472393 (-) 951 WP_006647266.1 glutathione synthase -
  ACN08L_RS02890 (ACN08L_02885) rsmE 472412..473143 (-) 732 WP_045069248.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACN08L_RS02895 (ACN08L_02890) - 473291..474064 (-) 774 WP_419237167.1 endonuclease -
  ACN08L_RS02900 (ACN08L_02895) - 474192..474677 (-) 486 WP_305371559.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41309.47 Da        Isoelectric Point: 6.4411

>NTDB_id=1104994 ACN08L_RS02870 WP_008987965.1 468887..469990(+) (pilU) [Photobacterium leiognathi subsp. mandapamensis strain Sr3.21]
MNLDYILAQMVERKASDLYITVGSPCLLKVDGNLHNLGELLDREAVDALFDEAMDDEQRKVFVTSREANFAIVRNGCRFR
LSAFWQRELPGVVIRRIETKIPNIHDLHLPIDMERLALSKRGLVLVVGATGSGKSTSMAAMTGYRNQNRSGHILTVEDPI
EFIHEHNKCIITQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIPDANGIGRHGVYELLLNTPRVADLIRRGELHEIKDVMAKSNQAGMVTFDQSLY
ALFRDGKITEQDALHHADSPNDLRLMIKMGDPNNKTASSLSGVKLDL

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=1104994 ACN08L_RS02870 WP_008987965.1 468887..469990(+) (pilU) [Photobacterium leiognathi subsp. mandapamensis strain Sr3.21]
ATGAATTTAGATTACATCTTAGCGCAGATGGTTGAGCGTAAAGCATCGGATTTATATATCACGGTCGGCTCGCCTTGCTT
GCTTAAAGTCGATGGTAACTTACACAACTTAGGTGAGTTATTAGATCGTGAAGCGGTCGATGCCTTGTTTGATGAAGCGA
TGGATGATGAGCAGCGCAAGGTATTTGTCACCAGTCGTGAGGCAAATTTTGCGATCGTGCGTAACGGTTGTCGTTTTCGT
TTAAGTGCATTTTGGCAACGTGAATTACCGGGTGTGGTGATCCGTCGAATTGAAACCAAGATCCCGAATATTCATGATCT
TCACCTACCTATTGATATGGAGCGTTTAGCGCTATCTAAACGTGGTTTAGTGTTGGTTGTGGGGGCTACGGGCTCAGGTA
AATCGACGTCGATGGCTGCAATGACGGGCTATCGAAACCAAAACCGTAGTGGCCATATTCTAACGGTAGAAGATCCGATT
GAATTTATTCACGAACATAACAAATGCATCATTACTCAACGTGAAGTGGGACTTGATACTGAAAGTTATGAAGTAGCACT
GAAAAACTCGCTGCGCCAAGCACCAGACATGATTTTGATCGGTGAGATCCGTACCCGTGAAACCATGGAATACGCAATGA
CCTTTGCTGAAACAGGTCACTTGTGTATGGCAACGCTACACGCCAACAATGCTAACCAAGCCCTTGAGCGTATTTTGCAT
TTAGTACCTAAAGAGCGTCGAGATCAGTTCTTATTTGATCTGTCATTGAATCTTAAATGTATTTTAGCGCAGCAATTGAT
CCCAGATGCGAATGGTATTGGTCGTCATGGTGTGTATGAGCTATTACTCAATACACCACGTGTTGCTGATTTGATCCGTC
GTGGTGAGCTACATGAAATTAAAGATGTCATGGCGAAATCAAACCAAGCGGGCATGGTGACATTTGACCAATCACTGTAT
GCCTTGTTCCGTGACGGTAAGATCACAGAGCAAGATGCACTGCACCATGCTGACTCACCAAATGATCTGCGTTTGATGAT
TAAAATGGGCGATCCAAACAATAAAACGGCGAGCTCGTTATCCGGCGTTAAATTAGATTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U1P8H5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

70.572

100

0.706
  pilU Pseudomonas stutzeri DSM 10701

60.112

97.003

0.583
  pilU Acinetobacter baylyi ADP1

55.307

97.548

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

92.643

0.395
  pilT Legionella pneumophila strain Lp02

40.476

91.553

0.371
  pilT Legionella pneumophila strain ERS1305867

40.476

91.553

0.371
  pilT Acinetobacter baumannii D1279779

39.42

94.005

0.371
  pilT Acinetobacter nosocomialis M2

39.42

94.005

0.371
  pilT Acinetobacter baumannii strain A118

39.42

94.005

0.371
  pilT Acinetobacter baylyi ADP1

39.13

94.005

0.368

Multiple sequence alignment