Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNQFN_RS20460 Genome accession   NZ_CP183038
Coordinates   4225497..4226540 (+) Length   347 a.a.
NCBI ID   WP_068635320.1    Uniprot ID   -
Organism   Thauera butanivorans strain TB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4220497..4231540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNQFN_RS20435 (ACNQFN_20435) - 4221185..4222462 (+) 1278 WP_068635325.1 dihydroorotase -
  ACNQFN_RS20440 (ACNQFN_20440) - 4222604..4223185 (+) 582 WP_068635324.1 Uma2 family endonuclease -
  ACNQFN_RS20445 (ACNQFN_20445) - 4223199..4223780 (-) 582 WP_068635323.1 YggT family protein -
  ACNQFN_RS20450 (ACNQFN_20450) proC 4223787..4224611 (-) 825 WP_068635322.1 pyrroline-5-carboxylate reductase -
  ACNQFN_RS20455 (ACNQFN_20455) - 4224679..4225404 (-) 726 WP_418647411.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNQFN_RS20460 (ACNQFN_20460) pilT 4225497..4226540 (+) 1044 WP_068635320.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNQFN_RS20465 (ACNQFN_20465) pilU 4226617..4227750 (+) 1134 WP_068635319.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNQFN_RS20470 (ACNQFN_20470) - 4227754..4228923 (-) 1170 WP_418647412.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACNQFN_RS20475 (ACNQFN_20475) - 4229191..4230798 (+) 1608 WP_418647413.1 FecR domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38597.33 Da        Isoelectric Point: 6.8283

>NTDB_id=1103748 ACNQFN_RS20460 WP_068635320.1 4225497..4226540(+) (pilT) [Thauera butanivorans strain TB]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQFEETWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRAIPSKVLTLEELNCPKIFKEIADTPRGIVLVTGPTGSGKSTTLAAMIDYINENEYGHILTVED
PIEFVHESKRCLINQREVHRDTMSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LADLVRRNIVSPVEARLRAQNKDSIAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1103748 ACNQFN_RS20460 WP_068635320.1 4225497..4226540(+) (pilT) [Thauera butanivorans strain TB]
ATGGACATCACCGAACTGCTCGCCTTCGCCGTCAAGAACAAGGCTTCCGACCTGCACCTTTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCCATGGCGACGTGCGCCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACGCCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGCAGTTCGAGGAAACCTGGGAGTGCGACTTCTCCTTCGCCGTGCCCAACCTCGCCCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGGGCCATTCCGTCCAAGGTGCTGACGCTCGA
GGAGCTGAACTGCCCGAAGATCTTCAAGGAGATCGCCGACACCCCGCGCGGCATCGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACGAGAACGAGTACGGCCACATCCTGACCGTCGAGGAC
CCGATCGAATTCGTGCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGATGTCCTTCAACAA
CGCCCTGCGCGCCTCGCTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCCGCGGCCGAGAAGGACATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCCAGGGCCGCGTCGCCGCGCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTGGACCAGTGC
CTGGCCGACCTGGTGCGGCGCAACATCGTATCGCCGGTCGAAGCGCGCCTGCGCGCCCAGAACAAGGACAGCATCGCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.71

99.424

0.793
  pilT Acinetobacter baumannii strain A118

79.71

99.424

0.793
  pilT Acinetobacter nosocomialis M2

79.71

99.424

0.793
  pilT Acinetobacter baylyi ADP1

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

77.941

97.983

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Legionella pneumophila strain Lp02

74.269

98.559

0.732
  pilT Legionella pneumophila strain ERS1305867

74.269

98.559

0.732
  pilT Neisseria meningitidis 8013

71.387

99.712

0.712
  pilT Neisseria gonorrhoeae MS11

71.098

99.712

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.857

96.83

0.657
  pilT Vibrio cholerae strain A1552

67.857

96.83

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.985

95.389

0.524
  pilU Acinetobacter baylyi ADP1

43.195

97.406

0.421
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.029

99.424

0.418

Multiple sequence alignment