Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNQFN_RS20465 Genome accession   NZ_CP183038
Coordinates   4226617..4227750 (+) Length   377 a.a.
NCBI ID   WP_068635319.1    Uniprot ID   -
Organism   Thauera butanivorans strain TB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4221617..4232750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNQFN_RS20440 (ACNQFN_20440) - 4222604..4223185 (+) 582 WP_068635324.1 Uma2 family endonuclease -
  ACNQFN_RS20445 (ACNQFN_20445) - 4223199..4223780 (-) 582 WP_068635323.1 YggT family protein -
  ACNQFN_RS20450 (ACNQFN_20450) proC 4223787..4224611 (-) 825 WP_068635322.1 pyrroline-5-carboxylate reductase -
  ACNQFN_RS20455 (ACNQFN_20455) - 4224679..4225404 (-) 726 WP_418647411.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNQFN_RS20460 (ACNQFN_20460) pilT 4225497..4226540 (+) 1044 WP_068635320.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNQFN_RS20465 (ACNQFN_20465) pilU 4226617..4227750 (+) 1134 WP_068635319.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNQFN_RS20470 (ACNQFN_20470) - 4227754..4228923 (-) 1170 WP_418647412.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACNQFN_RS20475 (ACNQFN_20475) - 4229191..4230798 (+) 1608 WP_418647413.1 FecR domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42103.50 Da        Isoelectric Point: 6.5062

>NTDB_id=1103749 ACNQFN_RS20465 WP_068635319.1 4226617..4227750(+) (pilU) [Thauera butanivorans strain TB]
MERDQALKFMHDLLRLMLQKNGSDLFLTVGFPPAIKIDGRITPQSNQAMQPQHTAELARAIMNDRQAAEFEATKECNFGI
SPAGIGRFRANAFVQQGRVGLVLRTIPQRIPSLEELGMPAVLNDVAMSKRGLVVFVGGTGTGKTTSLAAMVDYRNENSYG
HIITVEDPIEFVHPHKSCIVTQREIGIDSDSWETALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRAMISQRLLPLKERKGRVPAVEVMLNSPLIADLIFKGNIPEIKEIMKRSREIG
MQTFDQALFDLYEAGRITYEDALRNADSINDLRLQIKLHSKLGEQPSGSGLQDLGLV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1103749 ACNQFN_RS20465 WP_068635319.1 4226617..4227750(+) (pilU) [Thauera butanivorans strain TB]
ATGGAACGCGACCAGGCACTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGTTCCGACCTGTTCCT
CACCGTCGGCTTCCCGCCGGCGATCAAGATCGACGGCCGCATCACGCCGCAATCCAACCAGGCGATGCAGCCCCAGCACA
CCGCCGAACTCGCGCGCGCGATCATGAACGACCGCCAGGCGGCCGAATTCGAGGCCACCAAGGAATGCAACTTCGGTATC
TCGCCGGCTGGCATCGGCCGCTTCCGCGCCAACGCCTTCGTGCAGCAGGGCAGGGTCGGGCTGGTGCTGCGCACCATCCC
GCAGCGCATTCCCAGCCTGGAAGAACTGGGCATGCCCGCGGTACTGAACGACGTGGCGATGAGCAAGCGCGGCCTGGTGG
TGTTCGTCGGCGGCACCGGCACCGGCAAGACGACTTCGCTCGCCGCCATGGTCGATTACCGCAACGAGAACAGCTACGGC
CACATCATCACGGTCGAGGATCCGATCGAATTCGTGCACCCGCACAAGAGCTGCATCGTCACCCAGCGCGAGATCGGCAT
CGACTCCGACTCATGGGAAACCGCACTGAAGAACACCTTGCGCCAGGCGCCGGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGACTACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGATGATCTCGCAGCGGCTGCTGCCGCTGAAGGAACGCAAGGGCCGCGTGCCGGCGGTGGAAGTGATGCTGAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCAACATCCCCGAGATCAAGGAGATCATGAAGCGTTCGCGCGAAATCGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGACCTCTACGAGGCCGGCAGGATCACTTACGAAGACGCGCTGCGCAACGCCGA
CTCCATCAACGACCTGCGCCTGCAGATCAAGCTCCACAGCAAGCTCGGCGAACAGCCGAGCGGCAGCGGCCTGCAGGACC
TCGGCCTGGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.037

92.573

0.584
  pilU Acinetobacter baylyi ADP1

58

92.838

0.538
  pilU Vibrio cholerae strain A1552

54.167

95.491

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

88.859

0.39
  pilT Acinetobacter baylyi ADP1

43.323

89.39

0.387
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.859

0.377
  pilT Pseudomonas aeruginosa PAK

42.09

88.859

0.374
  pilT Legionella pneumophila strain Lp02

42.296

87.798

0.371
  pilT Legionella pneumophila strain ERS1305867

42.296

87.798

0.371
  pilT Acinetobacter nosocomialis M2

41.994

87.798

0.369
  pilT Acinetobacter baumannii D1279779

41.994

87.798

0.369
  pilT Acinetobacter baumannii strain A118

41.994

87.798

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.175

83.554

0.361
  pilT Vibrio cholerae strain A1552

43.175

83.554

0.361

Multiple sequence alignment