Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNH6H_RS10945 Genome accession   NZ_CP182947
Coordinates   2427474..2428517 (+) Length   347 a.a.
NCBI ID   WP_418317597.1    Uniprot ID   -
Organism   Piscinibacter sakaiensis strain 201-F6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2422474..2433517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6H_RS10920 (ACNH6H_10920) - 2422957..2423625 (+) 669 WP_418317592.1 PhaM family polyhydroxyalkanoate granule multifunctional regulatory protein -
  ACNH6H_RS10925 (ACNH6H_10925) - 2423663..2424883 (+) 1221 WP_418317593.1 FIST signal transduction protein -
  ACNH6H_RS10930 (ACNH6H_10930) ubiA 2424960..2425868 (+) 909 WP_418317594.1 4-hydroxybenzoate octaprenyltransferase -
  ACNH6H_RS10935 (ACNH6H_10935) proC 2425875..2426684 (-) 810 WP_418317595.1 pyrroline-5-carboxylate reductase -
  ACNH6H_RS10940 (ACNH6H_10940) - 2426694..2427401 (-) 708 WP_418317596.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNH6H_RS10945 (ACNH6H_10945) pilT 2427474..2428517 (+) 1044 WP_418317597.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNH6H_RS10950 (ACNH6H_10950) pilU 2428584..2429720 (+) 1137 WP_418317599.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNH6H_RS10955 (ACNH6H_10955) - 2429737..2430630 (+) 894 WP_418317601.1 NAD(P)-dependent oxidoreductase -
  ACNH6H_RS10960 (ACNH6H_10960) - 2430890..2433058 (+) 2169 WP_418321284.1 type I secretion system permease/ATPase -
  ACNH6H_RS10965 (ACNH6H_10965) - 2433058..2433306 (+) 249 WP_418317603.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38081.85 Da        Isoelectric Point: 7.1069

>NTDB_id=1103412 ACNH6H_RS10945 WP_418317597.1 2427474..2428517(+) (pilT) [Piscinibacter sakaiensis strain 201-F6]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEHKDVHNMVYDIMNDTHRKVYEESLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNTPKVFAELALKPRGLVLVTGPTGSGKSTTLAAMVNHLNEAEYGHILTVED
PIEFVHESKKCLVNQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRVAAHEIMLGTPAIRNLIREAKIAQMYSSIQTGNSMGMQTLDQN
LADLVRKGVISPAEARSKAKLPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1103412 ACNH6H_RS10945 WP_418317597.1 2427474..2428517(+) (pilT) [Piscinibacter sakaiensis strain 201-F6]
ATGGACATCACCCAACTGCTGGCTTTTTCGGTCAAGAACAAGGCTTCGGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCCATGGCGACGTGCGCCGCATCAATGTCGACCCGTTGGAGCACAAGGATGTGCACAACATGGTCTACGACA
TCATGAACGACACGCACCGCAAGGTGTACGAGGAATCGCTGGAATGCGACTTCTCGTTCGAGATCCAGGGGCTGGCGCGG
TTCCGGGTCAATGCGTTCAACCAGAACCGCGGTGCCGGCGCGGTATTCCGCACCATCCCGAGCAAGATCCTGACGCTCGA
GCAGCTGAATACGCCGAAGGTTTTTGCCGAACTGGCGCTGAAGCCGCGTGGCCTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGCAAATCGACGACGCTGGCGGCGATGGTCAATCATCTGAACGAAGCCGAATACGGCCACATCCTGACGGTCGAGGAT
CCGATCGAATTTGTCCACGAAAGCAAGAAGTGCCTGGTCAACCAGCGCGAGGTCGGCCCGCACACGCTGTCGTTCAGCAA
TGCATTGCGTTCGGCACTGCGCGAGGACCCGGATGCGATCTTGGTCGGCGAGATGCGCGACCTCGAGACGATCCGCCTTG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTATTCGGCACGCTGCACACCAGTTCGGCTGCAAAGACGATCGACCGCATC
GTCGACGTCTTTCCGGCCGCTGAAAAGGAGATGGTGCGGGCGATGCTGTCGGAATCGCTGGTCGCGGTGATCTCGCAGAC
GCTGTGCAAGCTGAAGGATGGCAGCGGCCGCGTTGCCGCCCACGAGATCATGCTCGGCACACCGGCGATCCGCAACCTGA
TCCGCGAAGCGAAGATCGCGCAGATGTATTCGTCGATCCAGACCGGCAACAGCATGGGCATGCAGACGCTCGACCAGAAC
CTCGCCGACCTCGTCCGCAAAGGTGTCATTTCGCCGGCCGAGGCCCGGTCGAAGGCCAAGCTGCCGGAGAATTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

78.261

99.424

0.778
  pilT Pseudomonas stutzeri DSM 10701

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406

Multiple sequence alignment