Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNH6H_RS10950 Genome accession   NZ_CP182947
Coordinates   2428584..2429720 (+) Length   378 a.a.
NCBI ID   WP_418317599.1    Uniprot ID   -
Organism   Piscinibacter sakaiensis strain 201-F6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2423584..2434720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6H_RS10925 (ACNH6H_10925) - 2423663..2424883 (+) 1221 WP_418317593.1 FIST signal transduction protein -
  ACNH6H_RS10930 (ACNH6H_10930) ubiA 2424960..2425868 (+) 909 WP_418317594.1 4-hydroxybenzoate octaprenyltransferase -
  ACNH6H_RS10935 (ACNH6H_10935) proC 2425875..2426684 (-) 810 WP_418317595.1 pyrroline-5-carboxylate reductase -
  ACNH6H_RS10940 (ACNH6H_10940) - 2426694..2427401 (-) 708 WP_418317596.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNH6H_RS10945 (ACNH6H_10945) pilT 2427474..2428517 (+) 1044 WP_418317597.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNH6H_RS10950 (ACNH6H_10950) pilU 2428584..2429720 (+) 1137 WP_418317599.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNH6H_RS10955 (ACNH6H_10955) - 2429737..2430630 (+) 894 WP_418317601.1 NAD(P)-dependent oxidoreductase -
  ACNH6H_RS10960 (ACNH6H_10960) - 2430890..2433058 (+) 2169 WP_418321284.1 type I secretion system permease/ATPase -
  ACNH6H_RS10965 (ACNH6H_10965) - 2433058..2433306 (+) 249 WP_418317603.1 hypothetical protein -
  ACNH6H_RS10970 (ACNH6H_10970) istB 2433446..2434264 (-) 819 WP_418317604.1 IS21-like element helper ATPase IstB -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42035.43 Da        Isoelectric Point: 6.8293

>NTDB_id=1103413 ACNH6H_RS10950 WP_418317599.1 2428584..2429720(+) (pilU) [Piscinibacter sakaiensis strain 201-F6]
MERDQASKFVNDLLRLMVSRNGSDLFLTADFPPAIKVDGKVTKVSPQPLTGQHTLALARAVMNDKQAAEFERTKECNFAI
SPQGIGRFRCNAFVQQAHVGIVMRTIPQTLPTIDTLKLPEVLKEIAMTKRGLVIFVGATGSGKSTSLAAMVNYRNENSHG
HIITLEDPVEFVHPHKNCIITQREVGIDTEGWEPALKNALRQAPDVILMGEIRDRESMDHAVAFAETGHLCMATMHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKGLVSQRLLPLQEGKGRVAAVEVMLNSPLISDLIFKGDVTEIKEIMKRSRELG
MQTFDQSLFDLYEANQITLEDALRNADSLNDLRLNIKLNSKRAKNTDLSAGTEHLAIM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1103413 ACNH6H_RS10950 WP_418317599.1 2428584..2429720(+) (pilU) [Piscinibacter sakaiensis strain 201-F6]
ATGGAACGTGACCAGGCATCCAAATTCGTCAATGACCTGCTGCGCCTGATGGTCAGCCGCAACGGCTCCGACCTGTTCCT
GACCGCCGATTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTGACGAAGGTCTCGCCGCAGCCGCTGACCGGGCAGCACA
CGCTGGCACTCGCGCGCGCGGTGATGAACGACAAGCAGGCGGCCGAATTCGAGCGCACGAAGGAGTGCAATTTCGCGATC
TCGCCGCAGGGCATCGGCCGCTTCCGCTGCAATGCCTTCGTGCAGCAGGCGCATGTGGGCATCGTGATGCGGACGATCCC
GCAGACGCTGCCGACGATCGACACGCTGAAGCTGCCCGAGGTGCTGAAGGAAATCGCGATGACCAAACGCGGGCTGGTCA
TCTTCGTCGGCGCCACCGGCTCCGGCAAGAGCACCTCGCTGGCGGCGATGGTCAACTACCGCAACGAGAATTCGCACGGC
CACATCATCACGCTCGAAGACCCGGTCGAATTCGTCCACCCGCACAAGAACTGCATCATCACGCAGCGCGAGGTCGGCAT
CGATACAGAAGGCTGGGAGCCGGCGCTGAAAAATGCGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGTCGATGGACCATGCGGTGGCTTTTGCCGAGACTGGCCACCTCTGCATGGCAACGATGCACGCAAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGCGCAGCTGCTGATGGACTTGTCGCTGAACCT
GAAGGGCCTGGTCTCGCAACGGCTGCTGCCGCTGCAGGAGGGCAAGGGCCGGGTTGCCGCCGTCGAGGTGATGCTCAATT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCGACGTCACCGAGATCAAGGAGATCATGAAGCGCTCGCGCGAACTCGGC
ATGCAGACCTTCGACCAGAGCCTGTTCGACCTCTACGAAGCGAACCAGATCACGCTCGAGGATGCGCTGCGCAATGCCGA
CTCGCTGAACGACCTGCGGCTGAACATCAAGCTGAACAGCAAGCGGGCGAAGAATACCGACCTGTCGGCCGGTACCGAAC
ACCTGGCGATCATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

53.994

96.032

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.711

84.127

0.368
  pilT Vibrio cholerae strain A1552

43.711

84.127

0.368
  pilT Legionella pneumophila strain ERS1305867

41.641

87.037

0.362
  pilT Legionella pneumophila strain Lp02

41.641

87.037

0.362

Multiple sequence alignment