Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNH6B_RS13965 Genome accession   NZ_CP182944
Coordinates   3257747..3258784 (-) Length   345 a.a.
NCBI ID   WP_248970255.1    Uniprot ID   -
Organism   Shewanella basaltis strain KCTC 22121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3252747..3263784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6B_RS13940 (ACNH6B_13940) - 3252973..3253533 (-) 561 WP_248970250.1 YqgE/AlgH family protein -
  ACNH6B_RS13945 (ACNH6B_13945) yciH 3253562..3253891 (-) 330 WP_248970251.1 stress response translation initiation inhibitor YciH -
  ACNH6B_RS13950 (ACNH6B_13950) hemH 3254476..3255489 (-) 1014 WP_418357314.1 ferrochelatase -
  ACNH6B_RS13955 (ACNH6B_13955) - 3255776..3256315 (-) 540 WP_248970253.1 glutathione peroxidase -
  ACNH6B_RS13960 (ACNH6B_13960) pilU 3256623..3257735 (-) 1113 WP_248970254.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNH6B_RS13965 (ACNH6B_13965) pilT 3257747..3258784 (-) 1038 WP_248970255.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNH6B_RS13970 (ACNH6B_13970) - 3258812..3259498 (+) 687 WP_418357315.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNH6B_RS13975 (ACNH6B_13975) proC 3259746..3260564 (+) 819 WP_248970257.1 pyrroline-5-carboxylate reductase -
  ACNH6B_RS13980 (ACNH6B_13980) - 3260707..3261255 (+) 549 WP_248970258.1 YggT family protein -
  ACNH6B_RS13985 (ACNH6B_13985) - 3261359..3261814 (+) 456 WP_418357316.1 DUF4426 domain-containing protein -
  ACNH6B_RS13990 (ACNH6B_13990) rdgB 3261881..3262522 (+) 642 WP_248970260.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACNH6B_RS13995 (ACNH6B_13995) hemW 3262519..3263658 (+) 1140 WP_248970261.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38364.99 Da        Isoelectric Point: 6.9449

>NTDB_id=1103396 ACNH6B_RS13965 WP_248970255.1 3257747..3258784(-) (pilT) [Shewanella basaltis strain KCTC 22121]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAGAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAGMVDYINENRHEHILTIED
PIEFVHQNKQCLVNQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMHKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1103396 ACNH6B_RS13965 WP_248970255.1 3257747..3258784(-) (pilT) [Shewanella basaltis strain KCTC 22121]
ATGGAAATCACTGAATTACTCGCTTTTAGTGTAAAACACAAAGCATCAGATCTACACCTTTCAGCAGGTGTTTCTCCAAT
GATCCGTGTCGACGGTGAAGTGCGAAAAATTAACTTACCGGCGCTAGATCATCAAGGTGTTCATAGTCTTGTATATGACA
TCATGAATGATAAACAACGAAAAGACTATGAAGAACACCTCGAAATCGACTTTTCATTTGAGGTTCCCAATCTGGCTCGT
TTTCGTGTTAACGCATTTAACCAAGCCCGCGGAGCCGGCGCAGTATTTCGTACCATTCCGTCTGATATTTTGTCATTAGA
ACAACTTGGCGCGCCTGAGATTTTTAAAAAGATATCAAGCTTTCCACGTGGATTAGTATTGGTTACAGGCCCAACGGGTT
CGGGTAAATCAACCACCCTTGCAGGTATGGTCGACTATATTAACGAAAATCGCCACGAACACATTTTAACCATTGAAGAT
CCAATCGAATTCGTTCACCAAAATAAACAATGTTTGGTTAACCAACGTGAAGTACATAAGCATACTCACAGCTTTAATGC
CGCACTGCGTAGTGCACTGCGTGAAGACCCTGACGTTATTCTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGCCTTG
CCATGACCGCAGCCGAAACGGGTCACCTGGTTTTTGGTACCTTGCACACCACCTCAGCGGCAAAAACCATTGACCGTATT
GTCGACGTATTCCCCGAAGGTGAAAAGAGTATGGTACGCACTATGTTGTCTGAATCATTGCAGGCCGTTATTTCGCAAAC
TCTCATTAAAAAAATTGGCGGGGGCCGAGTGGCCGCACACGAAATTATGATGGGTACACCTGCCATTCGTAACCTTATCC
GTGAAGATAAAGTGGCTCAAATGTATTCAGCCATTCAAACCGGTATGGCACATGGTATGCAAACGCTCGATCAATGTTTA
CAAAACTTGGTTAATCGTGGCCTCATTACCCGTGAAGATGCTATGCATAAGAGCTCGAATAAACAAGCTAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.24

99.13

0.786
  pilT Acinetobacter baumannii D1279779

79.24

99.13

0.786
  pilT Acinetobacter baumannii strain A118

79.24

99.13

0.786
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

75.66

98.841

0.748
  pilT Legionella pneumophila strain ERS1305867

75.66

98.841

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.262

97.391

0.412
  pilU Vibrio cholerae strain A1552

41.642

98.841

0.412
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment