Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNH6B_RS13960 Genome accession   NZ_CP182944
Coordinates   3256623..3257735 (-) Length   370 a.a.
NCBI ID   WP_248970254.1    Uniprot ID   -
Organism   Shewanella basaltis strain KCTC 22121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3251623..3262735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6B_RS13935 (ACNH6B_13935) ruvX 3252118..3252537 (-) 420 WP_248972551.1 Holliday junction resolvase RuvX -
  ACNH6B_RS13940 (ACNH6B_13940) - 3252973..3253533 (-) 561 WP_248970250.1 YqgE/AlgH family protein -
  ACNH6B_RS13945 (ACNH6B_13945) yciH 3253562..3253891 (-) 330 WP_248970251.1 stress response translation initiation inhibitor YciH -
  ACNH6B_RS13950 (ACNH6B_13950) hemH 3254476..3255489 (-) 1014 WP_418357314.1 ferrochelatase -
  ACNH6B_RS13955 (ACNH6B_13955) - 3255776..3256315 (-) 540 WP_248970253.1 glutathione peroxidase -
  ACNH6B_RS13960 (ACNH6B_13960) pilU 3256623..3257735 (-) 1113 WP_248970254.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNH6B_RS13965 (ACNH6B_13965) pilT 3257747..3258784 (-) 1038 WP_248970255.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNH6B_RS13970 (ACNH6B_13970) - 3258812..3259498 (+) 687 WP_418357315.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNH6B_RS13975 (ACNH6B_13975) proC 3259746..3260564 (+) 819 WP_248970257.1 pyrroline-5-carboxylate reductase -
  ACNH6B_RS13980 (ACNH6B_13980) - 3260707..3261255 (+) 549 WP_248970258.1 YggT family protein -
  ACNH6B_RS13985 (ACNH6B_13985) - 3261359..3261814 (+) 456 WP_418357316.1 DUF4426 domain-containing protein -
  ACNH6B_RS13990 (ACNH6B_13990) rdgB 3261881..3262522 (+) 642 WP_248970260.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40868.82 Da        Isoelectric Point: 5.9318

>NTDB_id=1103395 ACNH6B_RS13960 WP_248970254.1 3256623..3257735(-) (pilU) [Shewanella basaltis strain KCTC 22121]
MDVRPFLTIMVEKKASDLFITAGFPPSAKIDGELRQLSETKLSPQQSLAFVESLMTDVQKKEFHDTSECNFAFAATGLGR
FRVSAFWQRESAGCVMRRIETDIPEVDDLKLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLIPKSDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMGKSREQGMQTFDQA
LLDLYLEGEISYADALHHADSPNDLRLMIKLQSNESTSAGSMEGITLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1103395 ACNH6B_RS13960 WP_248970254.1 3256623..3257735(-) (pilU) [Shewanella basaltis strain KCTC 22121]
ATGGATGTTCGACCATTTTTAACCATTATGGTAGAGAAAAAAGCCTCAGATTTATTCATTACAGCAGGTTTTCCACCCAG
TGCAAAAATTGATGGTGAGTTACGTCAGCTGTCAGAGACAAAACTCTCACCGCAACAGTCTTTAGCGTTTGTAGAATCAC
TAATGACTGATGTACAAAAAAAAGAGTTTCATGACACCAGCGAGTGTAACTTTGCATTTGCTGCTACAGGATTAGGCCGT
TTCCGGGTTAGTGCTTTTTGGCAACGTGAGTCAGCAGGATGCGTAATGCGTCGTATCGAAACCGATATTCCTGAAGTTGA
CGACTTAAAGCTGCCCGCCATCCTTAAAGACTTGGTAATGAGTAAGCGCGGCTTAATCATTATGGTTGGTGGTACTGGTA
CAGGTAAATCAACCTCGCTTGCCGCACTGGTTGGCTATCGTAATGCTCATGCCCGTGGCCACATTTTAACCATTGAAGAC
CCGGTTGAATTTGTACATAACCACCGCAAAAGTATTATTACTCAGCGCGAAGTCGGGATCGACACCGACTCATTTGACGC
GGCACTTAAAAGCTCATTGCGCCAAGCTCCAGATGTGATTTTAATTGGTGAAATCCGCACCCAAGAAACCATGGAGTTTG
CATTATCGTTTGCCGAAACCGGCCACCTATGTATGGCAACGTTACACGCAAACAACGCTAACCAAGCGCTTGATCGTATT
ATGCATTTAGTGCCAGAAAGTAAGCACAATCAATTGCTTTTCGATTTGTCGTTAAATCTGCGCGGTATTGTTGCCCAGCA
GCTTATTCCTAAGTCAGATGGTACAGGTCGTCGTGCGGCTATTGAGGTGCTTATTAATACCCCTCGTGTCGCCAGTTTGA
TTGCCAAAAACGAATTGCATTTACTAAAAGAAACAATGGGTAAATCACGCGAACAAGGCATGCAAACCTTTGACCAAGCC
TTATTAGATTTATACCTAGAAGGTGAAATCAGCTACGCAGATGCACTACACCATGCAGATTCCCCCAACGATTTACGTTT
AATGATTAAACTGCAAAGTAACGAGTCAACATCAGCAGGCTCAATGGAAGGGATCACCTTAGACATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.559

100

0.619
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilU Vibrio cholerae strain A1552

57.027

100

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

39.13

93.243

0.365

Multiple sequence alignment