Detailed information NTG1101263

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACNJS6_RS18130
• Genome accession: NZ_CP182336
• Coordinates: 3699067..3700047 (-)
• Length: 326 a.a.
• NCBI ID: WP_001326494.1
• Uniprot ID: D3GUL4
• Organism: Escherichia coli strain A134_1EC
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3694067..3705047

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNJS6_RS18100	yggI	3694722..3695219 (+)	498	WP_032306561.1	SprT family zinc-dependent metalloprotease	-
ACNJS6_RS18105	endA	3695314..3696021 (+)	708	WP_000286500.1	deoxyribonuclease I	-
ACNJS6_RS18110	rsmE	3696101..3696832 (+)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ACNJS6_RS18115	gshB	3696845..3697795 (+)	951	WP_000593273.1	glutathione synthase	-
ACNJS6_RS18120	yqgE	3697904..3698467 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
ACNJS6_RS18125	ruvX	3698467..3698883 (+)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
ACNJS6_RS18130	pilT	3699067..3700047 (-)	981	WP_001326494.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACNJS6_RS18135	yggS	3700065..3700769 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
ACNJS6_RS18140	yggT	3700787..3701353 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ACNJS6_RS18145	yggU	3701350..3701640 (+)	291	WP_000994920.1	DUF167 family protein YggU	-
ACNJS6_RS18150	rdgB	3701648..3702241 (+)	594	WP_001174777.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
ACNJS6_RS18155	hemW	3702234..3703370 (+)	1137	WP_000239943.1	radical SAM family heme chaperone HemW	-
ACNJS6_RS18160	yggM	3703525..3704532 (-)	1008	WP_000745210.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=1101263 ACNJS6_RS18130 WP_001326494.1 3699067..3700047(-) (pilT) [Escherichia coli strain A134_1EC]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1101263 ACNJS6_RS18130 WP_001326494.1 3699067..3700047(-) (pilT) [Escherichia coli strain A134_1EC]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: D3GUL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362