Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABXS94_RS03415 Genome accession   NZ_AP031566
Coordinates   737585..738622 (-) Length   345 a.a.
NCBI ID   WP_104852405.1    Uniprot ID   -
Organism   Acinetobacter towneri strain PT23-B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732585..743622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXS94_RS03385 (PT23B2_06540) - 733356..733811 (+) 456 WP_096902263.1 bacteriohemerythrin -
  ABXS94_RS03390 (PT23B2_06550) - 733959..735044 (+) 1086 WP_353675534.1 hypothetical protein -
  ABXS94_RS03395 (PT23B2_06560) - 735041..735361 (+) 321 WP_004975319.1 RnfH family protein -
  ABXS94_RS03400 (PT23B2_06570) bamE 735418..735816 (-) 399 WP_353675535.1 outer membrane protein assembly factor BamE -
  ABXS94_RS03405 (PT23B2_06580) fur 735935..736372 (+) 438 WP_004975325.1 ferric iron uptake transcriptional regulator -
  ABXS94_RS03410 (PT23B2_06590) pilU 736445..737563 (-) 1119 WP_004975326.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABXS94_RS03415 (PT23B2_06600) pilT 737585..738622 (-) 1038 WP_104852405.1 type IV pilus twitching motility protein PilT Machinery gene
  ABXS94_RS03420 (PT23B2_06610) - 738747..739436 (+) 690 WP_353675536.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABXS94_RS03425 - 739797..740072 (-) 276 WP_004975329.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38308.82 Da        Isoelectric Point: 6.3479

>NTDB_id=110001 ABXS94_RS03415 WP_104852405.1 737585..738622(-) (pilT) [Acinetobacter towneri strain PT23-B2]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPDVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMIDFINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQSLLKKNGGGRVAAHEIMIGIPAVRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=110001 ABXS94_RS03415 WP_104852405.1 737585..738622(-) (pilT) [Acinetobacter towneri strain PT23-B2]
ATGGATATTACAGAATTATTGGCGTTCTCCGCTAAAAACGGCGCTTCGGATTTACACTTATCTGCGGGCATGCCACCACT
GATTCGTGTGGATGGTGAAGTTCGTCGAATTAACTTACCAGCATTAGAACATAAAGAAGTACACAAATTAGTGTACGACA
TTATGAATGATAAACAACGTCGTGATTATGAAGAAAATCTAGAAACGGACTTTTCTTTTGAAGTCCCAGACGTGGCTCGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCTTCTAAAGTTTTGACCCTTGA
AGATTTAGGCATGGGACAAATCTTTAAAGATATTTGTGAATATCCACGTGGTTTGGTATTGGTGACAGGCCCAACGGGTT
CAGGTAAATCAACCACACTTGCCGCGATGATTGACTTTATTAATGACAATCGCTACGACCATATTCTGACCGTCGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGTTTGGTTAACCAACGAGAAGTACACCGTGATACGCATGGCTTTAACGA
AGCATTACGTTCGGCACTGCGTGAAGACCCTGACATTATTCTAGTGGGTGAGATGCGTGACTTAGAAACCATTCGTTTGG
CACTGACTGCTGCTGAAACGGGTCACTTGGTATTTGGTACGCTACACACCACCTCTGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGTTGTCTGAATCTTTACAAGCCGTCATTTCACAATC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTAGCTGCGCATGAAATCATGATTGGTATTCCTGCAGTGCGTAACCTGATTC
GTGAAAACAAAGTAGCTCAAATGTACTCGGCAATTCAGACTGGTGCCAACTACGGTATGACCACCCTTGACCAGAGTTTG
AAAAACTTGGTATCGCGCGGTGTGATTAGCCCACAAACGGCTCGTACGGTTGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.203

100

0.942
  pilT Acinetobacter baumannii strain A118

94.203

100

0.942
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.696

100

0.687
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment