Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABXS94_RS03410 Genome accession   NZ_AP031566
Coordinates   736445..737563 (-) Length   372 a.a.
NCBI ID   WP_004975326.1    Uniprot ID   A0A7Y8TYA5
Organism   Acinetobacter towneri strain PT23-B2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 731445..742563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXS94_RS03380 (PT23B2_06530) - 732244..733092 (+) 849 WP_096902262.1 class II glutamine amidotransferase -
  ABXS94_RS03385 (PT23B2_06540) - 733356..733811 (+) 456 WP_096902263.1 bacteriohemerythrin -
  ABXS94_RS03390 (PT23B2_06550) - 733959..735044 (+) 1086 WP_353675534.1 hypothetical protein -
  ABXS94_RS03395 (PT23B2_06560) - 735041..735361 (+) 321 WP_004975319.1 RnfH family protein -
  ABXS94_RS03400 (PT23B2_06570) bamE 735418..735816 (-) 399 WP_353675535.1 outer membrane protein assembly factor BamE -
  ABXS94_RS03405 (PT23B2_06580) fur 735935..736372 (+) 438 WP_004975325.1 ferric iron uptake transcriptional regulator -
  ABXS94_RS03410 (PT23B2_06590) pilU 736445..737563 (-) 1119 WP_004975326.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABXS94_RS03415 (PT23B2_06600) pilT 737585..738622 (-) 1038 WP_104852405.1 type IV pilus twitching motility protein PilT Machinery gene
  ABXS94_RS03420 (PT23B2_06610) - 738747..739436 (+) 690 WP_353675536.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABXS94_RS03425 - 739797..740072 (-) 276 WP_004975329.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41846.11 Da        Isoelectric Point: 6.7283

>NTDB_id=110000 ABXS94_RS03410 WP_004975326.1 736445..737563(-) (pilU) [Acinetobacter towneri strain PT23-B2]
MDFNDLLNLMIQQKASDLFITADVEPSMKIHGQIVPVAKSKLTGEIVGQLLNSIMSEKQRKEFAETRECNFAITNREKTA
RFRVSAFQQRDEPGMVLRRIETKIPTMDDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAIVAQQLIPTIDGNSRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGERLLHATSNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=110000 ABXS94_RS03410 WP_004975326.1 736445..737563(-) (pilU) [Acinetobacter towneri strain PT23-B2]
ATGGACTTTAATGATTTACTGAATTTAATGATTCAGCAAAAAGCGTCTGACTTGTTTATTACAGCCGATGTTGAACCTTC
CATGAAAATTCATGGACAAATTGTGCCTGTAGCCAAAAGTAAACTTACAGGTGAAATTGTCGGTCAGCTGCTTAACTCAA
TTATGAGTGAAAAACAGCGCAAGGAATTTGCCGAAACCCGTGAATGTAACTTTGCGATTACCAACCGTGAAAAAACAGCA
CGTTTCCGTGTCAGCGCATTCCAACAACGTGATGAGCCGGGCATGGTACTCCGTCGGATTGAAACCAAAATCCCGACCAT
GGATGACTTGAAACTGCCACCTATTCTCAAAGATTTGGCGATGACCAAACGTGGCATCATCATTTTTGTAGGTGCGACTG
GTACAGGTAAATCGACTTCTTTGGCGTCTATTATTGGTTACCGCAACCAAAACTCCAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTGCATGAACATGCAGGCTGTATTATTACCCAGCGTGAAGTCGGAATTGATACTGACTCCTTTGA
AATCGCCTTAAAAAATACTTTGCGTCAAGCACCTGACGTGATTCTGATTGGTGAGATTCGTTCCCGCGAAACCATGGATT
ATGCAATTGCCTTTGCCGAAACAGGTCACTTGGTATTTGCCACCCTGCACGCCAACAACGCTAACCAAGCAATTGACCGT
ATTATTCACTTCTTTGAAGCAGACCGACATAACCAGTTGTTCATGGATTTGTCGCTAAACCTGAAAGCGATTGTGGCACA
GCAATTAATTCCAACCATAGATGGCAACTCACGTCGTGCTGCGATTGAAATTTTAATTAACTCACCATTAATTGCAGATT
TAATCCGTAAAGGTGATGTGCACGAAATTAAAGACCTAATGAAGCGTTCTCGCGAATTGGGCATGCAAACCTTTGACCAA
GCTTTATACGACCTCTATAAAGCCAAGCAAATTAGCTACAAAGACGCACTTAAACACGCCGACTCGCCAAACGATTTGCG
TCTACAAATCAAATTATCAGAAGAAGGTGGCGAGCGTTTGTTACATGCAACCTCGAATATTACCTTCGATGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y8TYA5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

69.003

99.731

0.688
  pilU Vibrio cholerae strain A1552

56.125

94.355

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain Lp02

42.012

90.86

0.382
  pilT Legionella pneumophila strain ERS1305867

42.012

90.86

0.382
  pilT Acinetobacter nosocomialis M2

41.108

92.204

0.379
  pilT Acinetobacter baumannii D1279779

41.108

92.204

0.379
  pilT Acinetobacter baumannii strain A118

41.108

92.204

0.379
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Acinetobacter baylyi ADP1

39.942

92.204

0.368
  pilT Vibrio cholerae strain A1552

39.031

94.355

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.355

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.595

93.011

0.368
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Neisseria meningitidis 8013

39.017

93.011

0.363

Multiple sequence alignment