Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNANZ_RS02930 Genome accession   NZ_CP181370
Coordinates   608874..609917 (-) Length   347 a.a.
NCBI ID   WP_094435387.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain A12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 603874..614917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNANZ_RS02900 (ACNANZ_02900) - 604261..604659 (+) 399 WP_094435399.1 YraN family protein -
  ACNANZ_RS02905 (ACNANZ_02905) - 604720..605322 (+) 603 WP_094435397.1 SIS domain-containing protein -
  ACNANZ_RS02910 (ACNANZ_02910) - 605319..605984 (+) 666 WP_416401423.1 BON domain-containing protein -
  ACNANZ_RS02915 (ACNANZ_02915) - 606012..606944 (-) 933 WP_416401424.1 NAD(P)-dependent oxidoreductase -
  ACNANZ_RS02920 (ACNANZ_02920) pilU 607019..608155 (-) 1137 WP_094435391.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNANZ_RS02925 (ACNANZ_02925) - 608181..608810 (-) 630 WP_094435389.1 cyclic nucleotide-binding domain-containing protein -
  ACNANZ_RS02930 (ACNANZ_02930) pilT 608874..609917 (-) 1044 WP_094435387.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNANZ_RS02935 (ACNANZ_02935) - 609957..610679 (+) 723 WP_094435385.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNANZ_RS02940 (ACNANZ_02940) - 610720..611850 (+) 1131 WP_201397098.1 aminotransferase class V-fold PLP-dependent enzyme -
  ACNANZ_RS02945 (ACNANZ_02945) - 611911..612333 (-) 423 WP_094435381.1 DNA-binding protein -
  ACNANZ_RS02950 (ACNANZ_02950) - 612518..613819 (+) 1302 WP_201397099.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38192.98 Da        Isoelectric Point: 6.8355

>NTDB_id=1099868 ACNANZ_RS02930 WP_094435387.1 608874..609917(-) (pilT) [Alicycliphilus denitrificans strain A12]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMINHLNETEYGHILTIED
PIEFVHEAKKCLVNQREVGPMTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMTGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPSEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1099868 ACNANZ_RS02930 WP_094435387.1 608874..609917(-) (pilT) [Alicycliphilus denitrificans strain A12]
GTGGACATCACCCAGCTGCTCGCCTTCAGCGTGAAGAACAAGGCGTCCGACCTGCATCTGTCGGCCGGCCTTCCTCCGAT
GATCCGGGTGCATGGTGATGTGCGGCGCATCAACGTCGATGCGCTGGACCACAAGACCGTCCATGCCATGGTGTACGACA
TCATGAGCGATGCCCAGCGCAAGGTCTACGAAGAGTTCCTGGAGGTGGACTTCTCCTTCGAGATCGAGGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGCGGCGGTCTTCCGCACCATCCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATCTTCGCCGACCTGGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGATCAACCACCTCAACGAGACCGAGTACGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAGGCCAAGAAATGCCTGGTCAACCAGCGCGAGGTGGGGCCGATGACGCTGTCGTTCTCCGC
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTCGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CCATGACGGCGGCCGAGACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGAGGAGAAGGAGATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGACGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTCGGCATGCAGACGCTCGACCAGAAT
CTCACCGACCTGGTGCGCCGCAACATCATCAGCCCCTCAGAGGCGCGCAGCAAGGCCAAGATCCCGGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.582

96.542

0.421
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment