Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNANZ_RS02920 Genome accession   NZ_CP181370
Coordinates   607019..608155 (-) Length   378 a.a.
NCBI ID   WP_094435391.1    Uniprot ID   A0A3R7EG42
Organism   Alicycliphilus denitrificans strain A12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 602019..613155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNANZ_RS02890 (ACNANZ_02890) - 602403..603326 (+) 924 WP_416401421.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  ACNANZ_RS02895 (ACNANZ_02895) rsmI 603323..604261 (-) 939 WP_416401422.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACNANZ_RS02900 (ACNANZ_02900) - 604261..604659 (+) 399 WP_094435399.1 YraN family protein -
  ACNANZ_RS02905 (ACNANZ_02905) - 604720..605322 (+) 603 WP_094435397.1 SIS domain-containing protein -
  ACNANZ_RS02910 (ACNANZ_02910) - 605319..605984 (+) 666 WP_416401423.1 BON domain-containing protein -
  ACNANZ_RS02915 (ACNANZ_02915) - 606012..606944 (-) 933 WP_416401424.1 NAD(P)-dependent oxidoreductase -
  ACNANZ_RS02920 (ACNANZ_02920) pilU 607019..608155 (-) 1137 WP_094435391.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNANZ_RS02925 (ACNANZ_02925) - 608181..608810 (-) 630 WP_094435389.1 cyclic nucleotide-binding domain-containing protein -
  ACNANZ_RS02930 (ACNANZ_02930) pilT 608874..609917 (-) 1044 WP_094435387.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNANZ_RS02935 (ACNANZ_02935) - 609957..610679 (+) 723 WP_094435385.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNANZ_RS02940 (ACNANZ_02940) - 610720..611850 (+) 1131 WP_201397098.1 aminotransferase class V-fold PLP-dependent enzyme -
  ACNANZ_RS02945 (ACNANZ_02945) - 611911..612333 (-) 423 WP_094435381.1 DNA-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41646.11 Da        Isoelectric Point: 6.9077

>NTDB_id=1099867 ACNANZ_RS02920 WP_094435391.1 607019..608155(-) (pilU) [Alicycliphilus denitrificans strain A12]
MERDQASKFINDLLKLMVSRGGSDLFITADFPPAIKVDGKVTKVSPQPLTPVHTLTLARSIMSDKQVADFERTKECNFAI
APAGIGRFRANAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEIAMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIVNFFPEERRAQLLMDLSLNLKAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGDVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVISYEDALRNADSINDLRLQIKLGSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1099867 ACNANZ_RS02920 WP_094435391.1 607019..608155(-) (pilU) [Alicycliphilus denitrificans strain A12]
ATGGAACGCGACCAGGCCAGCAAATTCATCAACGACCTGCTCAAGCTGATGGTCAGCCGGGGCGGCAGCGACCTGTTCAT
CACGGCCGACTTTCCGCCGGCCATCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTCACGCCCGTGCACA
CGCTGACGCTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAACTTCGCCATA
GCCCCCGCGGGCATAGGGCGCTTTCGCGCCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGCTGCGCACCATCCC
GCTGACGCTGCCCACCATCGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAGATCGCGATGACCAAGCGCGGCCTGTGCA
TCCTCGTGGGCGCCACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAAAACTCCTTCGGC
CACATCATCACCGTCGAGGACCCCATCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAGGTGGGGCT
CGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTCATGGACCTGTCGCTGAACCT
CAAGGCCATGGTCTCGCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGCCGCGCGGCGGCCGTCGAGGTCATGCTCAACA
CCCCGCTGATCGCCGACCTGATCTTCAAGGGCGATGTCGCCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTGTTCGAGGCCAACGTCATCAGCTACGAGGACGCCCTGCGCAACGCCGA
CTCGATCAACGACCTGCGCCTGCAGATCAAGCTCGGCAGCCAGCGCGCCAAGACCACCGACCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R7EG42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368

Multiple sequence alignment