Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9T68_RS22925 Genome accession   NZ_CP181346
Coordinates   5069180..5070223 (+) Length   347 a.a.
NCBI ID   WP_280191479.1    Uniprot ID   -
Organism   Delftia sp. PS-11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5064180..5075223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9T68_RS22905 (H9T68_022900) - 5064609..5066213 (-) 1605 WP_280191482.1 AMP-binding protein -
  H9T68_RS22910 (H9T68_022905) - 5066379..5067107 (+) 729 WP_280191481.1 TetR/AcrR family transcriptional regulator -
  H9T68_RS22915 (H9T68_022910) - 5067138..5068391 (+) 1254 WP_418515197.1 branched-chain amino acid ABC transporter substrate-binding protein -
  H9T68_RS22920 (H9T68_022915) - 5068421..5069146 (-) 726 WP_280191513.1 YggS family pyridoxal phosphate-dependent enzyme -
  H9T68_RS22925 (H9T68_022920) pilT 5069180..5070223 (+) 1044 WP_280191479.1 type IV pilus twitching motility protein PilT Machinery gene
  H9T68_RS22930 (H9T68_022925) - 5070243..5070878 (+) 636 WP_280191478.1 cyclic nucleotide-binding domain-containing protein -
  H9T68_RS22935 (H9T68_022930) pilU 5070981..5072117 (+) 1137 WP_280191477.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H9T68_RS22940 (H9T68_022935) - 5072175..5073086 (+) 912 WP_280191476.1 NAD(P)-dependent oxidoreductase -
  H9T68_RS22945 (H9T68_022940) - 5073170..5073886 (-) 717 WP_280191475.1 BON domain-containing protein -
  H9T68_RS22950 (H9T68_022945) - 5073883..5074491 (-) 609 WP_280191474.1 SIS domain-containing protein -
  H9T68_RS22955 (H9T68_022950) - 5074566..5074991 (-) 426 WP_280191473.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38328.06 Da        Isoelectric Point: 6.8353

>NTDB_id=1099816 H9T68_RS22925 WP_280191479.1 5069180..5070223(+) (pilT) [Delftia sp. PS-11]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMTDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLVNQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQSIGMQTLDQN
LTDLVRRNLISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1099816 H9T68_RS22925 WP_280191479.1 5069180..5070223(+) (pilT) [Delftia sp. PS-11]
GTGGATATCACCCAACTGCTGGCATTCAGTGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCAGGGCTGCCGCCCAT
GATCCGTGTGCACGGCGACGTGCGCCGCATCAATGTGGACCCCCTGGACCACAAGACAGTGCACGCCATGGTCTACGACA
TCATGACGGACTCCCAGCGCAAGGCCTACGAGGAATTCCTGGAAGTGGACTTCTCGTTCGAGATCGAGGGCCTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGTACCGGGGCGCGGCCGCCGTATTCCGTACGATTCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTTGCCGACCTGGCACTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACAGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCCATCGAATTCGTGCACGACTCCAAGAAATGCCTGGTCAACCAGCGCGAGGTGGGGCCGATGACGCAGTCGTTCTCCGC
AGCGCTGCGCTCGGCACTGCGCGAAGACCCTGATGCCATCCTGGTCGGTGAAATGCGTGACCTCGAAACCATCCGGCTGG
CCATGACGGCGGCGGAAACCGGCCACCTGGTCTTCGGCACACTGCACACGTCGAGCGCGGCCAAGACCATTGACCGTATC
ATTGACGTGTTTCCGGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTCTCCGAATCGCTGCAGGCCGTGATTTCTCAGAC
GCTGTGCAAAACCAAGGATGGCCAGAGCCGTGTCGCGGCCCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCCCAGATGTACTCGACCATCCAGACCAGCCAGAGCATCGGCATGCAGACGCTGGACCAGAAC
CTCACCGACCTGGTGCGGCGCAACCTCATCAGCCCTGCCGAAGCGCGCAGCAAGGCCAAGATCCCGGAAAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter nosocomialis M2

75.072

99.424

0.746
  pilT Acinetobacter baumannii D1279779

75.072

99.424

0.746
  pilT Acinetobacter baumannii strain A118

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment