Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H9T68_RS22935 Genome accession   NZ_CP181346
Coordinates   5070981..5072117 (+) Length   378 a.a.
NCBI ID   WP_280191477.1    Uniprot ID   -
Organism   Delftia sp. PS-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5065981..5077117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9T68_RS22910 (H9T68_022905) - 5066379..5067107 (+) 729 WP_280191481.1 TetR/AcrR family transcriptional regulator -
  H9T68_RS22915 (H9T68_022910) - 5067138..5068391 (+) 1254 WP_418515197.1 branched-chain amino acid ABC transporter substrate-binding protein -
  H9T68_RS22920 (H9T68_022915) - 5068421..5069146 (-) 726 WP_280191513.1 YggS family pyridoxal phosphate-dependent enzyme -
  H9T68_RS22925 (H9T68_022920) pilT 5069180..5070223 (+) 1044 WP_280191479.1 type IV pilus twitching motility protein PilT Machinery gene
  H9T68_RS22930 (H9T68_022925) - 5070243..5070878 (+) 636 WP_280191478.1 cyclic nucleotide-binding domain-containing protein -
  H9T68_RS22935 (H9T68_022930) pilU 5070981..5072117 (+) 1137 WP_280191477.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H9T68_RS22940 (H9T68_022935) - 5072175..5073086 (+) 912 WP_280191476.1 NAD(P)-dependent oxidoreductase -
  H9T68_RS22945 (H9T68_022940) - 5073170..5073886 (-) 717 WP_280191475.1 BON domain-containing protein -
  H9T68_RS22950 (H9T68_022945) - 5073883..5074491 (-) 609 WP_280191474.1 SIS domain-containing protein -
  H9T68_RS22955 (H9T68_022950) - 5074566..5074991 (-) 426 WP_280191473.1 YraN family protein -
  H9T68_RS22960 (H9T68_022955) rsmI 5074991..5075932 (+) 942 WP_280191472.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41997.47 Da        Isoelectric Point: 6.9076

>NTDB_id=1099817 H9T68_RS22935 WP_280191477.1 5070981..5072117(+) (pilU) [Delftia sp. PS-11]
MERDQASKFINDLLKLMVSRSGSDLFITADFPPAIKIDGKVTKVSPQPLSPAHTLTLARSIMSDRQIADFERTKECNFAI
SPSGIGRFRVNAFIQQGRVGMVLRTIPVSLPTIDSLGVPQILKEVAMTKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIVTIEDPIEFVHLHKNCVVTQREVGLDTDSWDAALKNSLRQAPDVILMGEIRDRETMEHAIVFSETGHLCLATLHANSA
NQALDRIVNFFPEERRQQLLMDLSLNLRAMVSQRLLPKQDSKGRVAAIEVMLNSPLISDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDLFEANLITYEDALRNADSVNDLRLQIKLGSQRAKSLDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1099817 H9T68_RS22935 WP_280191477.1 5070981..5072117(+) (pilU) [Delftia sp. PS-11]
ATGGAAAGAGATCAGGCCAGTAAATTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAGCGGCAGCGACCTCTTCAT
CACGGCGGACTTCCCGCCGGCGATCAAGATCGACGGCAAGGTGACCAAGGTCTCGCCGCAGCCGCTGTCGCCCGCGCACA
CGCTGACGCTGGCGCGCTCCATCATGAGCGACCGGCAGATCGCCGACTTCGAGCGCACCAAGGAGTGCAATTTCGCGATC
TCGCCGTCCGGCATCGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTCGGCATGGTGCTGCGGACCATTCC
CGTATCGCTGCCCACCATCGACAGCCTGGGCGTGCCGCAGATCCTCAAGGAAGTGGCCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAATCGACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAAAACTCCTATGGC
CACATCGTGACCATCGAGGACCCCATCGAATTCGTGCACCTGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGACGCGGCGCTCAAGAACTCGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCATGCCATCGTCTTCTCGGAAACCGGCCACCTGTGCCTGGCGACACTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAACGGCGCCAGCAACTGCTCATGGACCTGTCGCTGAACCT
GCGCGCCATGGTTTCGCAGCGCCTGCTGCCCAAGCAAGACAGCAAGGGCCGCGTGGCAGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCACCGAGATCAAGGAAATCATGAAGAAAAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGCCAACCTGATCACCTATGAGGATGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGTCGCTGGACCTGGCGGCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.037

92.328

0.582
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

42.042

88.095

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365

Multiple sequence alignment