Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM67C_RS11595 Genome accession   NZ_CP181246
Coordinates   2447646..2448686 (+) Length   346 a.a.
NCBI ID   WP_066080992.1    Uniprot ID   A0A378UL22
Organism   Bergeriella denitrificans strain DSM 17675     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442646..2453686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67C_RS11580 (ACM67C_11580) recX 2444290..2444757 (+) 468 WP_066081499.1 recombination regulator RecX Regulator
  ACM67C_RS11585 (ACM67C_11585) rsgA 2445550..2446416 (-) 867 WP_066081152.1 ribosome small subunit-dependent GTPase A -
  ACM67C_RS11590 (ACM67C_11590) - 2446677..2447372 (-) 696 WP_066081155.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM67C_RS11595 (ACM67C_11595) pilT 2447646..2448686 (+) 1041 WP_066080992.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM67C_RS11600 (ACM67C_11600) - 2448831..2449997 (+) 1167 WP_066080997.1 PilT/PilU family type 4a pilus ATPase -
  ACM67C_RS11605 (ACM67C_11605) - 2450634..2451059 (+) 426 WP_066081404.1 CopD family protein -
  ACM67C_RS11610 (ACM67C_11610) - 2451076..2451555 (+) 480 WP_115225447.1 CYTH domain-containing protein -
  ACM67C_RS11615 (ACM67C_11615) - 2451853..2452341 (+) 489 WP_066081473.1 dihydrofolate reductase -
  ACM67C_RS11620 (ACM67C_11620) - 2452714..2453145 (+) 432 WP_066081451.1 surface-adhesin E family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38189.92 Da        Isoelectric Point: 7.3372

>NTDB_id=1099555 ACM67C_RS11595 WP_066080992.1 2447646..2448686(+) (pilT) [Bergeriella denitrificans strain DSM 17675]
MQMTDLLAFSVKNQASDLHLSAGMPPMIRVHGDIRRINLPQMPAEEVGDMLFSVMNEQQQRLFRQELEVDFSFELPNIAR
FRVNAFTTGRGPAAVFRTIPNAVLSLEALKAPRILQKIADNPRGLVLVTGPTGSGKSTTLAAMVDYINETQPAHILTIED
PVEFIHKSKKALINQRELHQHTLSFHNALRSALREDPDVILVGEMRDPETIRLALTAAETGHLVFATLHTTGAAKTIDRI
VDVFPAGEKEMVRTMLSESLRAVISQTLLKTRDGSGRVAAHEILVSTPAIRNLIRENKIAQIQSAIQTGQSHGMQTLDQS
LQSLLRQGLIDADTARSKAQYSDGML

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1099555 ACM67C_RS11595 WP_066080992.1 2447646..2448686(+) (pilT) [Bergeriella denitrificans strain DSM 17675]
ATGCAAATGACCGACTTACTCGCTTTCAGCGTAAAAAACCAGGCCTCCGACCTTCATCTGAGCGCGGGTATGCCGCCGAT
GATACGCGTACACGGCGATATCCGCCGCATCAATCTGCCGCAGATGCCGGCGGAAGAGGTGGGCGATATGCTGTTTTCGG
TGATGAACGAGCAGCAGCAGCGGCTGTTCCGCCAGGAATTGGAAGTAGATTTTTCCTTCGAGCTGCCCAATATTGCCCGC
TTCCGCGTGAATGCGTTTACCACGGGGCGCGGGCCGGCAGCGGTATTCCGCACCATTCCGAATGCGGTGTTGTCGCTGGA
AGCGCTCAAAGCGCCGCGCATCCTGCAAAAAATCGCCGATAATCCACGCGGGCTGGTTTTGGTCACGGGGCCGACCGGCT
CGGGCAAATCCACTACGCTGGCGGCCATGGTCGATTATATCAACGAGACCCAGCCGGCGCATATTCTCACCATCGAAGAC
CCCGTCGAATTTATCCACAAAAGCAAAAAAGCCCTGATTAACCAGCGCGAGCTGCACCAGCACACCCTCAGCTTCCACAA
TGCGCTGCGCTCGGCGCTGCGGGAAGACCCCGATGTGATTTTGGTGGGCGAAATGCGCGACCCGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCATCTGGTGTTTGCCACCCTGCACACCACCGGCGCGGCGAAAACCATAGACCGTATT
GTCGATGTGTTCCCCGCAGGCGAAAAAGAAATGGTCCGCACCATGCTTTCGGAGTCGCTGCGGGCGGTGATTTCGCAGAC
CCTGCTGAAAACCCGTGACGGCAGCGGCCGCGTGGCCGCGCACGAAATTCTGGTGTCCACGCCCGCGATACGCAACCTGA
TTCGGGAAAACAAAATCGCCCAAATCCAATCGGCCATCCAAACCGGCCAGAGCCACGGGATGCAAACCCTGGACCAATCG
CTGCAATCGCTGCTGCGCCAAGGGCTGATTGATGCCGATACCGCCCGCAGCAAAGCACAATACAGCGACGGTATGCTCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378UL22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

80

99.711

0.798
  pilT Neisseria meningitidis 8013

80

99.711

0.798
  pilT Acinetobacter baylyi ADP1

69.706

98.266

0.685
  pilT Acinetobacter baumannii D1279779

68.497

100

0.685
  pilT Acinetobacter baumannii strain A118

68.497

100

0.685
  pilT Acinetobacter nosocomialis M2

68.497

100

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.118

98.266

0.679
  pilT Pseudomonas aeruginosa PAK

67.941

98.266

0.668
  pilT Legionella pneumophila strain Lp02

64.602

97.977

0.633
  pilT Legionella pneumophila strain ERS1305867

64.602

97.977

0.633
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.118

98.266

0.63
  pilT Vibrio cholerae strain A1552

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.212

97.977

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.739

99.711

0.416
  pilU Vibrio cholerae strain A1552

40.854

94.798

0.387
  pilU Acinetobacter baylyi ADP1

40

93.931

0.376

Multiple sequence alignment