Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGHM3_RS02355 Genome accession   NZ_AP031496
Coordinates   510359..511393 (-) Length   344 a.a.
NCBI ID   WP_345419448.1    Uniprot ID   A0AAV3U0L5
Organism   Halioxenophilus aromaticivorans strain KU68F     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505359..516393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHM3_RS02330 (R50071_04640) gshB 505880..506827 (+) 948 WP_345419432.1 glutathione synthase -
  ACGHM3_RS02335 (R50071_04650) - 506985..507857 (+) 873 WP_345419435.1 energy transducer TonB -
  ACGHM3_RS02340 (R50071_04660) - 508000..508584 (+) 585 WP_345419438.1 YqgE/AlgH family protein -
  ACGHM3_RS02345 (R50071_04670) ruvX 508707..509156 (+) 450 WP_345419442.1 Holliday junction resolvase RuvX -
  ACGHM3_RS02350 (R50071_04680) pilU 509153..510295 (-) 1143 WP_345419445.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHM3_RS02355 (R50071_04690) pilT 510359..511393 (-) 1035 WP_345419448.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHM3_RS02360 (R50071_04700) - 511526..512215 (+) 690 WP_345419451.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHM3_RS02365 (R50071_04710) proC 512274..513116 (+) 843 WP_345419454.1 pyrroline-5-carboxylate reductase -
  ACGHM3_RS02370 (R50071_04720) - 513287..513883 (+) 597 WP_345419457.1 YggT family protein -
  ACGHM3_RS02375 (R50071_04730) - 514321..516303 (+) 1983 WP_345419461.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38137.86 Da        Isoelectric Point: 6.8322

>NTDB_id=109870 ACGHM3_RS02355 WP_345419448.1 510359..511393(-) (pilT) [Halioxenophilus aromaticivorans strain KU68F]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYDIMNDKQRKDYEEYLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEELGMGQVFRDIAMCPRGLVLVTGPTGSGKSTSLAAMVDYINNNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLQAVISQTLMKKVGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTMDQCL
QDLVAKRLVAREVAREKAKSPDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=109870 ACGHM3_RS02355 WP_345419448.1 510359..511393(-) (pilT) [Halioxenophilus aromaticivorans strain KU68F]
ATGGATATTACGGAGTTACTCGCCTTTAGTGCCAAACAAGGCGCCTCGGACCTCCACCTGTCGGCGGGCCTTCCGCCCAT
GATTCGTGTAGACGGCGATGTGCGCCGCATCAACCTGCCGCCCATGGAGCACAAGCAAGTGCACGGGTTGATCTACGACA
TCATGAACGACAAGCAACGTAAGGATTACGAAGAATACCTTGAAACCGACTTCTCGTTTGAGGTGCCAGGTGTAGCGCGG
TTCCGGGTCAATGCTTTTAACCAAAACCGTGGCTCCGGCGCTGTGTTCCGTACGATTCCCTCCAAGGTTTTAACCATGGA
AGAATTGGGCATGGGCCAAGTGTTTCGCGACATCGCCATGTGCCCCCGCGGCTTGGTGCTGGTAACCGGCCCCACCGGTA
GCGGTAAATCCACCAGCTTGGCGGCCATGGTGGACTACATCAACAACAACAAATACGAGCATATCCTCACCATTGAGGAC
CCCATCGAATTTGTACACGAATCGAAAAAATGCCTGGTGAACCAACGCGAAGTGCACCGTGACACCCACGGCTTTTCCGA
GGCACTGCGTTCGGCACTGCGAGAAGACCCGGACATTATTCTGGTGGGTGAGATGCGAGACTTGGAAACCATCCGTCTGG
CCCTCACCGCGGCCGAAACCGGCCACTTAGTGTTCGGCACCTTGCACACCACCTCGGCGGCAAAAACCATTGACCGGGTA
GTGGATGTATTCCCCGCCAACGAAAAAGCCATGGTGCGTTCAATGCTTTCGGAATCACTGCAGGCAGTAATTTCTCAGAC
ATTGATGAAAAAAGTCGGCGGCGGCCGGGTGGCTGCCCACGAGATTATGCGCGGCACACCGGCCATCCGAAACCTGATCC
GCGAGGACAAGGTCGCCCAGATGTACTCCGCCATCCAAACCGGCGGCTCACTAGGCATGCAAACCATGGATCAGTGCCTG
CAGGATTTGGTGGCCAAGCGCCTAGTAGCACGAGAAGTGGCGCGCGAAAAAGCCAAATCGCCGGATGCTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

90.116

100

0.901
  pilT Pseudomonas stutzeri DSM 10701

88.953

100

0.89
  pilT Acinetobacter baumannii D1279779

82.558

100

0.826
  pilT Acinetobacter baumannii strain A118

82.558

100

0.826
  pilT Acinetobacter nosocomialis M2

82.267

100

0.823
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

72.384

100

0.724
  pilT Legionella pneumophila strain ERS1305867

72.384

100

0.724
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.359

97.093

0.683
  pilT Vibrio cholerae strain A1552

70.359

97.093

0.683
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.709

0.404
  pilU Vibrio cholerae strain A1552

39.359

99.709

0.392
  pilU Acinetobacter baylyi ADP1

39.244

100

0.392

Multiple sequence alignment