Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHM3_RS02350 Genome accession   NZ_AP031496
Coordinates   509153..510295 (-) Length   380 a.a.
NCBI ID   WP_345419445.1    Uniprot ID   A0AAV3U0M4
Organism   Halioxenophilus aromaticivorans strain KU68F     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504153..515295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHM3_RS02320 (R50071_04620) pilH 504430..504792 (-) 363 WP_345419425.1 twitching motility response regulator PilH -
  ACGHM3_RS02325 (R50071_04630) pilG 505199..505594 (-) 396 WP_345419428.1 twitching motility response regulator PilG Regulator
  ACGHM3_RS02330 (R50071_04640) gshB 505880..506827 (+) 948 WP_345419432.1 glutathione synthase -
  ACGHM3_RS02335 (R50071_04650) - 506985..507857 (+) 873 WP_345419435.1 energy transducer TonB -
  ACGHM3_RS02340 (R50071_04660) - 508000..508584 (+) 585 WP_345419438.1 YqgE/AlgH family protein -
  ACGHM3_RS02345 (R50071_04670) ruvX 508707..509156 (+) 450 WP_345419442.1 Holliday junction resolvase RuvX -
  ACGHM3_RS02350 (R50071_04680) pilU 509153..510295 (-) 1143 WP_345419445.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHM3_RS02355 (R50071_04690) pilT 510359..511393 (-) 1035 WP_345419448.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHM3_RS02360 (R50071_04700) - 511526..512215 (+) 690 WP_345419451.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHM3_RS02365 (R50071_04710) proC 512274..513116 (+) 843 WP_345419454.1 pyrroline-5-carboxylate reductase -
  ACGHM3_RS02370 (R50071_04720) - 513287..513883 (+) 597 WP_345419457.1 YggT family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42316.61 Da        Isoelectric Point: 5.9417

>NTDB_id=109869 ACGHM3_RS02350 WP_345419445.1 509153..510295(-) (pilU) [Halioxenophilus aromaticivorans strain KU68F]
MEFDRLLALMVDKGASDLFITAGVPPSVKLHGKVTPVTTTPLSPEKTRELVLSVMNEKQRLEFLEQKELNFAISARGIGR
FRCSAFYQRNLAGMVLRRIETVIPKVDDLGLPDIIKDLAMTKRGLVMFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGLDTESFEIALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHGQLWMDLSLNLRGIIAQQLIPTPDGKGRRACLEVLLNSPLASDLIRKGEVSELKELMKKSSEIGMQTFDQA
LFELYDRGEITYEDALSHADSPNDLRLMIKLASETDAEYLGHAADELSLESDDSDRTKLY

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=109869 ACGHM3_RS02350 WP_345419445.1 509153..510295(-) (pilU) [Halioxenophilus aromaticivorans strain KU68F]
ATGGAGTTTGACCGCCTGCTGGCCCTGATGGTTGATAAAGGGGCCTCGGATTTATTTATCACCGCAGGTGTTCCACCCTC
GGTCAAGCTGCACGGCAAGGTCACCCCGGTTACCACCACGCCACTGTCGCCAGAAAAAACCCGCGAACTGGTGCTTAGCG
TAATGAACGAAAAACAGCGGTTGGAGTTTTTGGAGCAGAAAGAACTTAACTTTGCCATTAGCGCCCGCGGGATTGGCCGC
TTCCGCTGCTCGGCGTTTTATCAGCGCAATTTGGCCGGCATGGTACTGCGCCGCATTGAAACCGTAATTCCTAAAGTGGA
TGACCTGGGCCTGCCCGACATCATCAAAGATTTGGCCATGACCAAACGCGGCCTGGTGATGTTTGTGGGCGCCACCGGCA
CCGGTAAATCCACCTCGTTGGCGGCCATGATTGGCCACCGCAATCGCAATTCCAAGGGCCATATTATTTCCATTGAGGAC
CCCATCGAGTTTATTCATCAGCACCAGGGCTGCATTGTTACCCAGCGCGAGGTGGGCCTGGACACCGAATCCTTTGAGAT
TGCTCTAAAAAACACCCTGCGCCAGGCGCCGGATGTCATTTTGATTGGTGAGGTGCGCTCCCGTGAAACCATGGAGCACG
CCATCGCTTTCGCCGAAACCGGCCACCTGTGCCTGTGTACCTTACACGCAAACAACGCCAACCAAGCCTTGGACCGTATC
ATCCACTTCTTCCCCGCCGATCGTCACGGCCAGCTGTGGATGGATTTGTCGCTTAACCTACGCGGCATTATTGCTCAGCA
ATTAATTCCAACGCCCGACGGCAAGGGCCGCCGCGCCTGTTTGGAGGTGCTGCTGAATTCGCCCTTAGCGTCGGATTTAA
TTCGTAAGGGTGAGGTGTCGGAATTAAAAGAACTGATGAAAAAATCCAGCGAAATCGGCATGCAGACCTTCGACCAGGCG
CTATTTGAGCTCTACGACCGGGGGGAAATCACCTATGAAGATGCCCTCTCCCACGCCGACTCACCCAACGACCTGCGCTT
AATGATCAAACTGGCTTCGGAAACAGACGCCGAATATCTGGGCCACGCTGCAGATGAGCTCAGCCTAGAGAGCGACGACT
CCGATCGAACCAAACTGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.867

98.684

0.768
  pilU Acinetobacter baylyi ADP1

66.947

93.947

0.629
  pilU Vibrio cholerae strain A1552

57.143

92.105

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.318

92.632

0.411
  pilT Acinetobacter baumannii strain A118

40.351

90

0.363
  pilT Acinetobacter nosocomialis M2

40.351

90

0.363
  pilT Acinetobacter baumannii D1279779

40.351

90

0.363
  pilT Vibrio cholerae strain A1552

40.653

88.684

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.653

88.684

0.361

Multiple sequence alignment