Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM26W_RS00960 Genome accession   NZ_CP181044
Coordinates   209606..210640 (+) Length   344 a.a.
NCBI ID   WP_416139012.1    Uniprot ID   -
Organism   Halomonas sp. HK25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 204606..215640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM26W_RS00930 (ACM26W_00930) - 204977..205432 (-) 456 WP_416139006.1 DUF4426 domain-containing protein -
  ACM26W_RS00935 (ACM26W_00935) metW 205488..206084 (-) 597 WP_416140788.1 methionine biosynthesis protein MetW -
  ACM26W_RS00940 (ACM26W_00940) metX 206084..207241 (-) 1158 WP_416139007.1 homoserine O-succinyltransferase MetX -
  ACM26W_RS00945 (ACM26W_00945) - 207288..207869 (-) 582 WP_416139009.1 YggT family protein -
  ACM26W_RS00950 (ACM26W_00950) proC 207933..208757 (-) 825 WP_416139010.1 pyrroline-5-carboxylate reductase -
  ACM26W_RS00955 (ACM26W_00955) - 208820..209539 (-) 720 WP_416139011.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM26W_RS00960 (ACM26W_00960) pilT 209606..210640 (+) 1035 WP_416139012.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM26W_RS00965 (ACM26W_00965) pilU 210691..211833 (+) 1143 WP_416139013.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM26W_RS00970 (ACM26W_00970) - 211853..212992 (-) 1140 WP_416139014.1 anhydro-N-acetylmuramic acid kinase -
  ACM26W_RS00975 (ACM26W_00975) tyrS 213107..214306 (+) 1200 WP_416139015.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37901.52 Da        Isoelectric Point: 6.5955

>NTDB_id=1097880 ACM26W_RS00960 WP_416139012.1 209606..210640(+) (pilT) [Halomonas sp. HK25]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMADRQRRDYEEFFETDFSFEVPGVSR
FRVNVFNHARGAGAVFRTIPADVLTLEDLGLGTVFRQLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYEHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKSMVRSMLSESLQAVVSQALLKRMGGGRVAAHEILIATAAVRNLVREDKVAQIYSAIQTGGNLGMQTLDASL
AKLVADNVVARDEAQAKAKGMLAN

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1097880 ACM26W_RS00960 WP_416139012.1 209606..210640(+) (pilT) [Halomonas sp. HK25]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAACAGAAGGCCTCCGACCTGCATCTGTCGGCGGGTCTGCCGCCGAT
GATCCGGGTCGATGGCGACATTCGCCGCTTGAACGTGCCGGCCATGGAGGACCGCGAGGTCCGCAAGTTGATCTACGACA
TCATGGCCGACCGTCAGCGCCGTGACTACGAGGAGTTCTTCGAGACCGACTTCTCCTTCGAGGTGCCCGGCGTGTCGCGC
TTCCGCGTCAACGTCTTCAATCATGCCCGCGGCGCTGGGGCGGTGTTCCGTACTATCCCCGCCGACGTGCTGACGTTGGA
GGACCTCGGCCTCGGCACCGTCTTCCGCCAGCTCTCCATGCTGCCCCGCGGCCTGGTGCTGGTGACCGGTCCCACCGGGT
CGGGCAAGAGCACTACGCTGGCGGCGATGATCGATTACATCAACGACAACCGCTACGAACACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTCCACCGCAGCAAGCGCTGTCTGATCAACCAGCGTGAGGTGCACCGCGACACCCACGGCTTCGCTCC
GGCCCTGCGCAGCGCCCTGCGCGAGGACCCGGACGTGATCCTGGTGGGCGAGTTGCGTGACCTGGAGACCATCCGGCTGG
CACTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGGGATGAGAAGTCCATGGTGCGCTCGATGCTCTCCGAGTCGCTGCAGGCGGTGGTCTCCCAAGC
GCTGCTCAAGCGAATGGGAGGCGGCCGCGTGGCGGCCCACGAGATCCTGATCGCGACCGCGGCGGTGCGCAACCTGGTCC
GTGAGGACAAGGTCGCGCAGATCTACTCGGCCATCCAGACCGGCGGCAACCTGGGCATGCAGACCCTGGACGCCTCGCTG
GCCAAGCTGGTGGCGGACAACGTGGTGGCGCGCGACGAAGCGCAGGCCAAGGCCAAGGGCATGCTGGCGAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.171

98.547

0.77
  pilT Pseudomonas aeruginosa PAK

77.286

98.547

0.762
  pilT Acinetobacter baumannii D1279779

76.401

98.547

0.753
  pilT Acinetobacter baumannii strain A118

76.401

98.547

0.753
  pilT Acinetobacter nosocomialis M2

76.106

98.547

0.75
  pilT Acinetobacter baylyi ADP1

75.811

98.547

0.747
  pilT Legionella pneumophila strain Lp02

71.302

98.256

0.701
  pilT Legionella pneumophila strain ERS1305867

71.302

98.256

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

65.882

98.837

0.651
  pilT Neisseria gonorrhoeae MS11

65.588

98.837

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.212

98.547

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

40.308

94.477

0.381
  pilU Vibrio cholerae strain A1552

39.104

97.384

0.381

Multiple sequence alignment