Detailed information NTG1097881

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACM26W_RS00965
• Genome accession: NZ_CP181044
• Coordinates: 210691..211833 (+)
• Length: 380 a.a.
• NCBI ID: WP_416139013.1
• Uniprot ID: -
• Organism: Halomonas sp. HK25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 205691..216833

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM26W_RS00940 (ACM26W_00940)	metX	206084..207241 (-)	1158	WP_416139007.1	homoserine O-succinyltransferase MetX	-
ACM26W_RS00945 (ACM26W_00945)	-	207288..207869 (-)	582	WP_416139009.1	YggT family protein	-
ACM26W_RS00950 (ACM26W_00950)	proC	207933..208757 (-)	825	WP_416139010.1	pyrroline-5-carboxylate reductase	-
ACM26W_RS00955 (ACM26W_00955)	-	208820..209539 (-)	720	WP_416139011.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACM26W_RS00960 (ACM26W_00960)	pilT	209606..210640 (+)	1035	WP_416139012.1	type IV pilus twitching motility protein PilT	Machinery gene
ACM26W_RS00965 (ACM26W_00965)	pilU	210691..211833 (+)	1143	WP_416139013.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACM26W_RS00970 (ACM26W_00970)	-	211853..212992 (-)	1140	WP_416139014.1	anhydro-N-acetylmuramic acid kinase	-
ACM26W_RS00975 (ACM26W_00975)	tyrS	213107..214306 (+)	1200	WP_416139015.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42123.63 Da
• Isoelectric Point: 6.1889

>NTDB_id=1097881 ACM26W_RS00965 WP_416139013.1 210691..211833(+) (pilU) [Halomonas sp. HK25]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGKLTPMGDQGLTVEQVNELVGASIPEAVMARFQVEREANFALSLK
DKGRFRVSAFQQRNQKAMVIRRIAFEIPHLEELSLPPVLGELANIKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKAIINQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIINFFPMERHGQVWLDLSLNLRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLIRKGEVNEIKNVMARSRDLGMQTF
DQALFDIFKAGKITEEVALVHADSANDLRMMIKYGDAGSEGMTQAREAASHLSLKRDDEF

Nucleotide

• Length: 1143 bp

>NTDB_id=1097881 ACM26W_RS00965 WP_416139013.1 210691..211833(+) (pilU) [Halomonas sp. HK25]
ATGACGGCGAAAGAGTGGCTCTACCAGCTGCTGGATATCATGGTGCAGAAGGAGGCATCGGATCTGCTGATTTCGGTGAA
TGCGCCGCCGACCCTCAAGATGGCCGGCAAGCTGACACCCATGGGGGACCAGGGGCTGACCGTGGAGCAGGTCAACGAGC
TGGTCGGCGCGTCGATTCCGGAAGCGGTGATGGCGCGCTTCCAGGTCGAGCGCGAGGCCAACTTCGCACTCAGCCTCAAG
GACAAGGGGCGCTTTCGCGTCAGCGCCTTCCAGCAGCGCAACCAGAAGGCCATGGTGATCCGGCGTATCGCCTTCGAGAT
CCCCCATCTGGAGGAGCTGTCGCTGCCCCCGGTGCTGGGCGAACTGGCCAATATCAAGCGAGGCCTGGTGTTCGTGGTGG
GCGGCACTGGTACGGGCAAGTCGACCACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCCTGGGCGGCCATATCATC
AGCGTCGAGGATCCCATCGAGTACGTGCACCCGCACAAGAAGGCGATCATCAATCAGCGCGAGGTCGGGATCGATACGGA
CTCCTTCGAGGTGGCGCTCAAGAACACCCTGCGCCAGGCACCCGATGTCATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAGCACGCGCTGACCTTCGCCGAGACCGGCCACCTCTGCCTGGCCACCCTGCACGCCAACAATGCCAACCAGGCC
CTTGATCGCATCATCAACTTCTTTCCCATGGAGCGTCACGGCCAGGTATGGCTTGACCTGTCGCTCAACTTGCGCGCCAT
CGTGGCCCAGCAGCTGCTGCCCACGGTAGATGGCGGGCGCTGCCCGGCGATCGAGATCATGCTCAAATCGCCGCTGATCG
GCGACCTGATACGTAAAGGCGAGGTCAACGAGATCAAGAACGTCATGGCCCGCTCCCGTGATCTGGGCATGCAGACCTTC
GATCAGGCGCTCTTCGATATCTTCAAGGCGGGCAAGATCACCGAAGAGGTGGCGCTGGTCCATGCCGACTCGGCCAACGA
TCTGCGCATGATGATCAAGTACGGGGACGCGGGCAGCGAGGGGATGACCCAGGCCCGTGAGGCCGCCAGTCACCTCTCGC
TGAAGCGCGACGACGAGTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (65-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.484	97.895	0.592
pilU	Vibrio cholerae strain A1552	57.895	95	0.55
pilU	Acinetobacter baylyi ADP1	54.348	96.842	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.131	100	0.421