Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM0O0_RS14550 Genome accession   NZ_CP180689
Coordinates   3118976..3120013 (+) Length   345 a.a.
NCBI ID   WP_081175033.1    Uniprot ID   -
Organism   Acinetobacter lactucae isolate nanjing     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3113976..3125013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0O0_RS14540 - 3114360..3117956 (+) 3597 WP_416040611.1 AAA family ATPase -
  ACM0O0_RS14545 - 3118157..3118849 (-) 693 WP_373357915.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM0O0_RS14550 pilT 3118976..3120013 (+) 1038 WP_081175033.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM0O0_RS14555 pilU 3120034..3121152 (+) 1119 WP_005803129.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM0O0_RS14560 fur 3121222..3121659 (-) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  ACM0O0_RS14565 - 3121771..3122169 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  ACM0O0_RS14570 - 3122234..3122548 (-) 315 WP_016144536.1 RnfH family protein -
  ACM0O0_RS14575 - 3122548..3123615 (-) 1068 WP_373357917.1 hypothetical protein -
  ACM0O0_RS14580 - 3123769..3124224 (-) 456 WP_002121698.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38302.90 Da        Isoelectric Point: 6.7269

>NTDB_id=1097468 ACM0O0_RS14550 WP_081175033.1 3118976..3120013(+) (pilT) [Acinetobacter lactucae isolate nanjing]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1097468 ACM0O0_RS14550 WP_081175033.1 3118976..3120013(+) (pilT) [Acinetobacter lactucae isolate nanjing]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACTTGTCTGCTGGCATGCCACCAAT
GATTCGTGTTGATGGCGAAGTCCGTCGCATTAACTTACCTGCTCTAGATCATAAAGATGTTCACCGTCTGGTCTATGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTGCCTAATGTTGCGCGT
TTCCGTGTTAATGCATTTAATCAAAACCGTGGTGCCGGTGCAGTGTTTCGTACCATTCCCTCCAAAGTGCTCAGCATGGA
AGATTTAGGCCTAGGTCAAATCTTTAAAGATATTTGTGATTATCCACGTGGCATTGTACTGGTTACAGGTCCAACAGGTT
CAGGTAAATCCACCACACTTGCAGCGATGATGGACTACATTAATGAAAACCGTTATGACCACATTTTAACGGTTGAAGAC
CCTATCGAGTTTGTACACCAGTCTAAGAAATGTCTCATCAACCAACGTGAAGTGCATCGAGACACACACGGTTTTAATGA
AGCGCTACGTTCAGCACTGCGTGAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CGCTTACTGCTGCCGAAACAGGCCATTTGGTGTTTGGTACGCTCCATACCACTTCTGCTGCAAAAACTATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGAGCCATGCTCTCTGAATCTTTACAGGCTGTTATCTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGGCGTGTTGCAGCCCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAGGTCGCGCAAATGTACTCAGCCATTCAGACTGGTGCGAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGTTTAGTCGCTCGCGGTGTAATCAGCCCGCAAACGGCTCGTACTGCTGCTAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter nosocomialis M2

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.101

0.391

Multiple sequence alignment